@article{citeulike:485803,
	title = {Homogeneous amperometric immunoassay for theophylline in whole blood.},
	address = {Department of Clinical Biochemistry, Medical School, University of Newcastle upon Tyne, UK.},
	author = {D. Athey and C. J. Mcneil and W. R. Bailey and H. J. Hager and W. H. Mullen and L. J. Russell},
	journal = {Biosensors \& bioelectronics},
	number = {9-10},
	pages = {415--419},
	url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=8311938},
	volume = {8},
	year = {1993},
	biburl = {http://www.bibsonomy.org/bibtex/2597b10f86d6b4d6ce55b25f329a0475e/biblio24},
	abstract = {A homogeneous spectrophotometric EMIT immunoassay kit for the quantitation of theophylline in serum or plasma has been modified to produce a rapid, amperometric immunoassay requiring a 50 microliters whole blood sample. The basis of the detection system for the assay is the electrochemical oxidation of NADH produced by G6PDH-labelled theophylline at a potential of +150 mV vs Ag/AgCl using platinised activated carbon (PACE) electrodes. Comparison of the amperometric whole blood method with the conventional spectrophotometric plasma assay produced a reasonable correlation: y = 0.90x - 1.01, (r = 0.98, n = 12). The advantage of the new method is that simple and robust instrumentation can rapidly determine theophylline in whole blood with no sample pre-treatment or separation steps.},
	issn = {0956-5663}, doi = {10.1016/0956-5663(93)80026-L }, citeulike-article-id = {485803}, priority = {2},
	keywords = {ag_detection competition electrochemistry emit g6pdh homogeneous immunoassay immunosensor labeled_enzyme }
}