@article{citeulike:797292,
	title = {Enzyme-amplified immunoassays.},
	address = {IQ(Bio) Ltd., Downham House, Downham's Lane, Milton Road, Cambridge CB4 1XG, England.},
	author = {C. J. Stanley and D. H. Ellis and D. L. Bates and A. Johannsson},
	journal = {J Pharm Biomed Anal},
	number = {8},
	pages = {811--820},
	url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=16867457},
	volume = {5},
	year = {1987},
	biburl = {http://www.bibsonomy.org/bibtex/2345d6989aa2456d27650de5b1c6776e8/biblio24},
	abstract = {The sensitivity of enzyme immunoassays may be enhanced by the use of enzyme-amplification. This technique uses the enzyme label in the immunoassay to provide a trigger substance for a secondary system that can generate a large quantity of coloured product. Two examples of enzyme amplifiers are described, using either a substrate cycle with phosphorylated hexose sugars, or a redox cycle involving the coenzyme NAD(+). The redox enzyme-amplifier has a detection limit of less than one attomole for the enzyme label, alkaline phosphatase. The limited dynamic range of enzyme-amplified immunoassays may be overcome by kinetic analysis of the colour development in the enzyme-amplifier, to add at least a further order of magnitude to the range of directly measured analyte concentrations in the immunoassay. This is illustrated in an enzyme-amplified immunoassay for human thyroid stimulating hormone. Amperometric measurement of the enzyme-amplifier provides a method to extend the dynamic range still further and compares favourably with the performance of a gamma counter, a luminometer or a fluorimeter.},
	issn = {0731-7085}, citeulike-article-id = {797292}, priority = {2},
	keywords = {electrochemistry enzyme immunoassay nad }
}