@article{Post_1992_49,
  abstract = {Calcium in the myocardial cell is highly compartmentalized and a fast,
	an intermediate, a slow and a nonexchangeable calcium pool have been
	described. The fast pool contains 66\% of the total cell exchangeable
	calcium in cultured neonatal rat heart cells with a t1/2 of less
	than 1.5 sec. Though the cellular origin of this fast pool is unknown,
	its rapidity and its displacement by La3+ most likely places it at
	the sarcolemma or at least in rapid equilibrium with the sarcolemma.
	We isolated the sarcolemma of cultured neonatal rat heart cells using
	the gas-dissection technique, which yields a pure sarcolemmal preparation
	in less than a second, thereby precluding membrane changes which
	might occur during conventional plasma membrane isolation. We determined
	the calcium binding characteristics of these membranes, using an
	on-line technique to monitor 45Ca, which allows measurement of 45Ca
	binding characteristics in the presence of unbound 45Ca. Two classes
	of calcium binding sites were determined: (i) Kd of 13 microM, capacity
	7 nmol/mg and (ii) Kd of 1.1 mM, capacity of 84 nmol/mg. To assess
	the molecular origin of the sarcolemmal calcium binding we treated
	the membranes with a variety of enzymes. Protease or neuraminidase
	treatment did not cause large changes in these parameters. Simultaneous
	treatment with two different phospholipases C or the extraction of
	the lipids with isopropanol resulted in a dramatic loss of the low-affinity
	binding sites. These results, in association with previously defined
	sarcolemmal phospholipid distribution, places the low-affinity binding
	sites at the cytoplasmic leaflet. The physiological implication of
	this localization as it pertains to cellular calcium exchange is
	discussed.},
  added-at = {2009-06-03T11:20:58.000+0200},
  author = {Post, J. A. and Langer, G. A.},
  biburl = {http://www.bibsonomy.org/bibtex/26a4500946e8ad7f676f9fbe60c496922/hake},
  description = {The whole bibliography file I use.},
  doi = {10.1007/BF00232054},
  file = {Post_1992_49.pdf:Post_1992_49.pdf:PDF},
  interhash = {edb3fbd3ad6dd5bd18c31bd1635ba96e},
  intrahash = {6a4500946e8ad7f676f9fbe60c496922},
  journal = {J. Membr. Biol.},
  key = 237,
  keywords = {1-Propanol, 1404340 Animals, Binding C, Calcium Calcium, Cell Cells, Compartmentation, Cultured, Dissection, Endopeptidases, Gov't, Lanthanum, Models, Myocardium, Neuraminidase, Newborn, Nifedipine, Nitrogen, Non-U.S. P.H.S., Phospholipase Phospholipids, Radioisotopes, Rats, Research Reticulu, Ryanodine, Sarcolemma, Sarcoplasmic Sites, Support, Terpenes, Thapsigargin, Theoretical, U.S. m,},
  month = Jul,
  number = 1,
  pages = {49--57},
  pmid = {1404340},
  timestamp = {2009-06-03T11:20:58.000+0200},
  title = {Sarcolemmal calcium binding sites in heart: I. Molecular origin in
	"gas-dissected" sarcolemma.},
  url = {http://dx.doi.org/10.1007/BF00232054},
  volume = 129,
  year = 1992
}