@article{citeulike:478705,
	title = {Enzyme-linked immunoadsorbent assay with electrochemical detection for alpha 1-acid glycoprotein.},
	author = {M. J. Doyle and H. B. Halsall and W. R. Heineman},
	journal = {Analytical Chemistry},
	month = {November},
	number = {13},
	pages = {2355--2360},
	url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=6517326},
	volume = {56},
	year = {1984},
	biburl = {http://www.bibsonomy.org/bibtex/26af97f6caec1891e4273b1b54b6ebc2b/biblio24},
	abstract = {An enzyme-linked lmmunoadsorbent voltammetrlc assay based on the detection of catalytically generated electroactlve product has been Investlgated. Human orosomucold (OMD, a,-acid glycoprotein) was cross-llnked to alkaline phosphatase (AP, EC 3.1.3.1) uslng glutaraldehyde. The physlcal, chemical, and lmmunologlcal properties of the OMD-AP conjugate compared favorably with those of the natlve precursors. AP-generated phenol was detected amperometrlcally at a carbon paste electrode by llquld chromatography/ electrochemlstry (LCEC). The detectlon limlt for OMD Is 1.0 WmL, and the assay Is most senSnlve between 1.0 and 10.0 ng/mL. The LCEC method exhiblts a 3-5% relatlve standard devlatlon.},
	issn = {0003-2700}, doi = {10.1021/ac00277a022}, citeulike-article-id = {478705}, priority = {2},
	keywords = {ag_detection competition electrochemistry heterogeneous immunoassay immunosensor }
}