@article{citeulike:613282,
	title = {Fluorescein isothiocyanate-hapten immunoassay for determination of peptide-cell interactions.},
	address = {Center for Molecular Imaging Research, Massachusetts General Hospital, Charlestown, MA 02129, USA.},
	author = {K. A. Kelly and F. Reynolds and R. Weissleder and L. Josephson},
	journal = {Anal Biochem},
	month = {July},
	number = {2},
	pages = {181--185},
	url = {http://dx.doi.org/10.1016/j.ab.2004.02.035},
	volume = {330},
	year = {2004},
	biburl = {http://www.bibsonomy.org/bibtex/2d34211c1a9187fc638cd2d76639209cd/biblio24},
	abstract = {We have developed a fluorescein isothiocyanate (FITC)-hapten immunoassay, where a FITC-labeled peptide binding to a cell is assayed as the amount of immunoreactive fluorescein present in a cell lysate. An antifluorescein-horseradish peroxidase conjugate binds to either a fluoresceinated peptide in the lysate or a fluorescein attached to the wells of a microtiter plate in a competitive fashion. After washing, solid-phase peroxidase activity is measured and inversely related to the amount of FITC-labeled peptide present. To demonstrate the assay, the interaction of a FITC-labeled bombesin-like peptide with the gastrin-releasing peptide receptor on PC-3 and HT-29 cells was investigated. Using PC-3 cells, we obtained similar displacement curves and numbers of binding sites per cell by both the FITC-hapten immunoassay and a reference radioreceptor assay. The FITC-hapten immunoassay is a sensitive and versatile method, since the same commercially available reagents can be used to assess interactions between any peptide and any receptor. In addition, the FITC-labeled peptide can be used to visualize receptors in fluorescent-activated cell sorting or fluorescent microscopy.},
	issn = {0003-2697}, doi = {10.1016/j.ab.2004.02.035}, citeulike-article-id = {613282}, priority = {2},
	keywords = {competition displacement immunoassay peptide }
}