@article{wagner_complex_2008, abstract = {Two barley populations, i.e. 135 doubled haploid {(DH)} lines of the cross {'Igri'} (rrs1) x {'Triton'} {(Rrs1)} {(I} x T) and 76 {DH} lines of the cross {'Post'} x {'Vixen'} (both rrs1) {(P} x V), were analysed to identify {QTL} for Rhynchosporium secalis resistance independent of the Rrs1 locus by using the single spore R. secalis isolate 271 {(Rrs1-virulent).} A major {QTL} with its positive allele derived from cv. {'Triton'} was detected in the I x T population on chromosome {2HS} explaining almost 80\% of the phenotypic variance. Thus, it can be considered as an R-gene corresponding to the already described {Rrs15(CI8288)} on chromosome {2HS.} In addition, two minor {QTL} were identified, one in the centromeric region of {6H} in a highly polymorphic region with already several mapped R-genes and a second one at the end of the short arm of chromosome {7H} which may be an allele of Rrs2 because of its chromosomal position. Regarding the {DH} population P x V different minor {QTL} were identified on chromosomes {6H} and {7H.} The first one is corresponding to the genomic region of the Rrs13 gene whereas the {QTL} on chromosome {7H} maps in a genomic region where several R-genes against different pathogens have been localized. A comparison of both {QTL} analyses reveals no R. secalis isolate 271-specific resistance locus but leads to the hypothesis that two of the identified {QTL} may be alleles of the R-genes {Rrs15(CI8288)} and Rrs2.}, added-at = {2009-02-06T09:11:55.000+0100}, author = {Wagner and Schweizer, G and Krämer, M and {Dehmer-Badani}, A G and Ordon, F and Friedt, W}, biburl = {http://www.bibsonomy.org/bibtex/29c783afbf4c20c836427a08b45e3408f/ipzgiessen}, doi = {10.1007/s00122-008-0881-5}, interhash = {1f9a65362f17bd64f1508fa3f6466252}, intrahash = {9c783afbf4c20c836427a08b45e3408f}, issn = {0040-5752}, journal = {{TAG.} Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik}, keywords = {imported}, month = {December}, note = {{PMID:} 18806993}, number = 1, pages = {113--22}, timestamp = {2009-02-06T09:11:55.000+0100}, title = {The complex quantitative {barley-Rhynchosporium} secalis interaction: newly identified {QTL} may represent already known resistance genes}, url = {http://www.ncbi.nlm.nih.gov/pubmed/18806993}, volume = 118, year = 2008 } @article{rygulla_identification_2008, abstract = {Verticillium longisporum is one of the major pathogens of oilseed rape {(Brassica} napus; genome {AACC,} 2n = 38) in Europe. Current European cultivars possess only a low level of resistance against V. longisporum, meaning that heavy infection can cause major yield losses. The aim of this study was to identify quantitative trait loci {(QTL)} for resistance against V. longisporum as a starting point for marker-assisted breeding of resistant cultivars. Resistance {QTL} were localized in a segregating oilseed rape population of 163 doubled haploid {(DH)} lines derived by microspore culture from the F1 of a cross between two B. napus breeding lines, one of which exhibited V. longisporum resistance derived by pedigree selection from a resynthesized B. napus genotype. A genetic map was constructed comprising 165 restriction fragment length polymorphism, 94 amplified fragment length polymorphism and 45 simple sequence repeats {(SSR)} markers covering a total of 1,739 {cM} on 19 linkage groups. Seedlings of the {DH} lines and parents were inoculated with V. longisporum isolates in four greenhouse experiments performed in Sweden during autumn 1999. In three of the experiments the {DH} lines were inoculated with a mixture of five isolates, while in the fourth experiment only one of the isolates was used. The intention was to simulate four different environments with variable disease pressure, while still maintaining uniform conditions in each environment to enable reliable disease scoring. The disease index {(DI)} was calculated by scoring symptoms on a total of 21 inoculated plants per line in comparison to 21 noninoculated plants per line. Using the composite interval mapping procedure a total of four different chromosome regions could be identified that showed significant {QTL} for resistance in more than one environment. Two major {QTL} regions were identified on the C-genome linkage groups N14 and N15, respectively; each of these {QTL} consistently exhibited significant effects on resistance in multiple environments. The presence of flanking markers for the respective {QTL} was associated with a significant reduction in {DI} in the inoculated {DH} lines.}, added-at = {2009-02-06T09:11:55.000+0100}, author = {Rygulla and Snowdon, R J and Friedt, W and Happstadius, I and Cheung, W Y and Chen, D}, biburl = {http://www.bibsonomy.org/bibtex/2e77ea7d66c38382061de3375967e333f/ipzgiessen}, doi = {{10.1094/PHYTO-98-2-0215}}, interhash = {80e4a90a6496385393f72da56d9f57b3}, intrahash = {e77ea7d66c38382061de3375967e333f}, issn = {{0031-949X}}, journal = {Phytopathology}, keywords = {imported}, month = {February}, note = {{PMID:} 18943198}, number = 2, pages = {215--21}, timestamp = {2009-02-06T09:11:55.000+0100}, title = {Identification of quantitative trait loci for resistance against Verticillium longisporum in oilseed rape {(Brassica} napus)}, url = {http://www.ncbi.nlm.nih.gov/pubmed/18943198}, volume = 98, year = 2008 } @article{hasan_association_2008, abstract = {Breeding of oilseed rape {(Brassica} napus ssp. napus) has evoked a strong bottleneck selection towards double-low (00) seed quality with zero erucic acid and low seed glucosinolate content. The resulting reduction of genetic variability in elite 00-quality oilseed rape is particularly relevant with regard to the development of genetically diverse heterotic pools for hybrid breeding. In contrast, B. napus genotypes containing high levels of erucic acid and seed glucosinolates (++ quality) represent a comparatively genetically divergent source of germplasm. Seed glucosinolate content is a complex quantitative trait, however, meaning that the introgression of novel germplasm from this gene pool requires recurrent backcrossing to avoid linkage drag for high glucosinolate content. Molecular markers for key low-glucosinolate alleles could potentially improve the selection process. The aim of this study was to identify potentially gene-linked markers for important seed glucosinolate loci via structure-based allele-trait association studies in genetically diverse B. napus genotypes. The analyses included a set of new simple-sequence repeat {(SSR)} markers whose orthologs in Arabidopsis thaliana are physically closely linked to promising candidate genes for glucosinolate biosynthesis. We found evidence that four genes involved in the biosynthesis of indole, aliphatic and aromatic glucosinolates might be associated with known quantitative trait loci for total seed glucosinolate content in B. napus. Markers linked to homoeologous loci of these genes in the paleopolyploid B. napus genome were found to be associated with a significant effect on the seed glucosinolate content. This example shows the potential of {Arabidopsis-Brassica} comparative genome analysis for synteny-based identification of gene-linked {SSR} markers that can potentially be used in marker-assisted selection for an important trait in oilseed rape.}, added-at = {2009-02-06T09:11:55.000+0100}, author = {Hasan and Friedt, W and {Pons-Kühnemann}, J and Freitag, N and Link, K and Snowdon, R}, biburl = {http://www.bibsonomy.org/bibtex/2ac347e9417d320e1715ec5b262e1264d/ipzgiessen}, doi = {10.1007/s00122-008-0733-3}, interhash = {aa2e6dd89a6ec199d47dd1315c72beef}, intrahash = {ac347e9417d320e1715ec5b262e1264d}, issn = {0040-5752}, journal = {{TAG.} Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik}, keywords = {imported}, month = {March}, note = {{PMID:} 18322671}, timestamp = {2009-02-06T09:11:55.000+0100}, title = {Association of gene-linked {SSR} markers to seed glucosinolate content in oilseed rape {(Brassica} napus ssp. napus)}, url = {http://www.ncbi.nlm.nih.gov/pubmed/18322671}, year = 2008 } @article{wagner_complex_2008, abstract = {Two barley populations, i.e. 135 doubled haploid (DH) lines of the cross 'Igri' (rrs1) x 'Triton' (Rrs1) (I x T) and 76 DH lines of the cross 'Post' x 'Vixen' (both rrs1) (P x V), were analysed to identify QTL for Rhynchosporium secalis resistance independent of the Rrs1 locus by using the single spore R. secalis isolate 271 (Rrs1-virulent). A major QTL with its positive allele derived from cv. 'Triton' was detected in the I x T population on chromosome 2HS explaining almost 80\% of the phenotypic variance. Thus, it can be considered as an R-gene corresponding to the already described Rrs15(CI8288) on chromosome 2HS. In addition, two minor QTL were identified, one in the centromeric region of 6H in a highly polymorphic region with already several mapped R-genes and a second one at the end of the short arm of chromosome 7H which may be an allele of Rrs2 because of its chromosomal position. Regarding the DH population P x V different minor QTL were identified on chromosomes 6H and 7H. The first one is corresponding to the genomic region of the Rrs13 gene whereas the QTL on chromosome 7H maps in a genomic region where several R-genes against different pathogens have been localized. A comparison of both QTL analyses reveals no R. secalis isolate 271-specific resistance locus but leads to the hypothesis that two of the identified QTL may be alleles of the R-genes Rrs15(CI8288) and Rrs2.}, added-at = {2008-11-18T12:07:18.000+0100}, author = {Wagner and Schweizer, G and Krämer, M and Dehmer-Badani, A G and Ordon, F and Friedt, W}, biburl = {http://www.bibsonomy.org/bibtex/201ceca59291d4cff5d168191d3d3ce65/ipzgiessen}, doi = {10.1007/s00122-008-0881-5}, interhash = {1f9a65362f17bd64f1508fa3f6466252}, intrahash = {01ceca59291d4cff5d168191d3d3ce65}, issn = {0040-5752}, journal = {TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik}, keywords = {imported}, month = {December}, note = {PMID: 18806993}, pages = {113-22}, timestamp = {2008-11-18T12:07:18.000+0100}, title = {The complex quantitative barley-Rhynchosporium secalis interaction: newly identified QTL may represent already known resistance genes}, url = {http://www.ncbi.nlm.nih.gov/pubmed/18806993}, volume = 118, year = 2008 } @article{ISI:000258488200006, abstract = {Field trials with a population of 108 doubled haploid (DH) lines of bread wheat (Triticum aestivum L.) derived from a cross between the Chinese winter wheat cultivars CA9613 and H1488 were carried out at Beijing (China) in 2000/2001 and 2001/2002. In addition, a field trial and a pot experiment were carried out at the experimental field stations of Giessen University (Germany) in the vegetation periods 2004/ 2005 and 2006/2007. Phenotypic data for major agronomic yield-related traits, i.e. grain weight per ear (GWE), grain number per ear (GNE), plant height and thousand-grain weight (TGW), were recorded in all experiments. In addition, biomass weight per tiller and ear weight were evaluated in the two field trials at Beijing. Based on the phenotypic data and a genetic map comprising 168 SSR markers, an analysis of quantitative trait loci (QTL) was carried out for yield and yield parameters using the composite interval mapping (CIM) approach. A total of 30 QTL were detected for these tr}, added-at = {2008-11-06T15:12:28.000+0100}, author = {Hai, Lin and Guo, Huijun and Wagner, Carola and Xiao, Shihe and Friedt, Wolfgang}, biburl = {http://www.bibsonomy.org/bibtex/20337f1890ce87e133596c942c2450e11/ipzgiessen}, interhash = {8261c1a047511d8a49ce345d16741819}, intrahash = {0337f1890ce87e133596c942c2450e11}, issn = {0168-9452}, journal = {PLANT SCIENCE}, keywords = {biomass_weight bread_wheat_(Triticum_aestivum_L.) doubled_haploids_(DHs) grain_weight quantitative_trait_loci_(QTL)}, number = 3, pages = {226-232}, timestamp = {2008-11-06T15:12:28.000+0100}, title = {Genomic regions for yield and yield parameters in Chinese winter wheat (Triticum aestivum L.) genotypes tested under varying environments correspond to QTL in widely different wheat materials}, volume = 175, year = 2008 } @article{ISI:000245359100002, abstract = {"Smart breeding", sometimes also called "clever breeding" or "precision breeding". stands for methodical alternatives to genetic engineering in modern plant breeding. Classical methods - such as crossbreeding of different parent varieties and Subsequent selection of progeny - are combined with novel molecular biological approaches. Smart breeding uses molecular-biological methods (e.g. gene mapping, mutagenesis) to characterize initial plants with desirable breeding properties. Methods of vegetable tissue culturing and subsequent regeneration of intact plants essentially reduce the steps among classical crossbreeding, selection of desirable breeding results and subsequent propagation. The breeding targets, such as e.g. resistance to diseases and pest infestation, stress tolerance (e.g. against dryness, salt, heat) or changed components (e.g. fatty acid pattern) are lastly achieved by means of classical breeding, i.e. by combination of suitable parent varieties, thus leading to useful, not genetically engineered plants.}, added-at = {2008-06-04T14:37:55.000+0200}, author = {Friedt, Wolfgang}, biburl = {http://www.bibsonomy.org/bibtex/230435d7d17c44e4b3c5c09749f7e308f/ipzgiessen}, interhash = {f8cff604b8a402a1b2ade6e33efacfdd}, intrahash = {30435d7d17c44e4b3c5c09749f7e308f}, issn = {0174-0008}, journal = {ERNAHRUNGS-UMSCHAU}, keywords = {IFZ imported}, number = 3, pages = {108+}, timestamp = {2008-06-04T14:37:55.000+0200}, title = {Smart breeding}, volume = 54, year = 2007 } @article{ISI:000247239100001, abstract = {Genetic diversity in spring bread wheat (T.aestivum L.) was studied in a total of 69 accessions. For this purpose, 52 microsatellite (SSR) markers were used and a total of 406 alleles were detected, of which 182 (44.8%) occurred at a frequency of < 5% (rare alleles). The number of alleles per locus ranged from 2 to 14 with an average of 7.81. The largest number of alleles per locus occurred in the B genome (8.65) as compared to the A (8.43) and D (5.93) genomes, respectively. The polymorphism index content (PIC) value varied from 0.24 to 0.89 with an average of 0.68. The highest PIC for all accessions was found in the B genome (0.71) as compared to the A (0.68) and D genomes (0.63). Genetic distance-based method (standard UPGMA clustering) and a model-based method (structure analysis) were used for cluster analysis. The two methods led to analogical results. Analysis of molecular variance (AMOVA) showed that 80.6% of the total variation could be explained by the variance within the geographical groups. In comparison}, added-at = {2008-06-04T14:34:51.000+0200}, author = {Hai, Lin and Wagner, Carola and Friedt, Wolfgang}, biburl = {http://www.bibsonomy.org/bibtex/2c878c6af1b0a05ea365cdb93d343ea0a/ipzgiessen}, interhash = {65ca08fc92a6dc6f7ca41d49335586fb}, intrahash = {c878c6af1b0a05ea365cdb93d343ea0a}, issn = {0016-6707}, journal = {GENETICA}, keywords = {IFZ SSRs Triticum aestivum_L. genetic_diversity microsatellites quantitative_structure_analysis spring_bread_wheat}, number = 3, pages = {213-225}, timestamp = {2008-06-04T14:34:51.000+0200}, title = {Quantitative structure analysis of genetic diversity among spring bread wheats (Triticum aestivum L.) from different geographical regions}, volume = 130, year = 2007 } @article{ISI:000250071900004, abstract = {Fonio millets (Digitaria exilis Stapf, D. iburua Stapf) are valuable indigenous staple food crops in West Africa. In order to investigate the genetic diversity and population differentiation in these millets, a total of 122 accessions from five countries (Benin, Burkina Faso, Guinea, Mali and Togo) were analysed by Amplified Fragment Length Polymorphisms (AFLPs). Genetic distance-based UPGMA clustering and principal coordinate analysis revealed a clear-cut differentiation between the two species and a clustering of D. exilis accessions in three major genetic groups fitting to their geographical origins.}, added-at = {2008-06-04T14:34:51.000+0200}, author = {Adoukonou-Sagbadja, H. and Wagner, C. and Dansi, A. and Ahlemeyer, J. and Daienou, O. and Akpagana, K. and Ordon, F. and Friedt, W.}, biburl = {http://www.bibsonomy.org/bibtex/288dd0f4b761e43e15111a8ebae15a09e/ipzgiessen}, interhash = {4c9c5c4c54fcdd8a05b99f7327a2bcc4}, intrahash = {88dd0f4b761e43e15111a8ebae15a09e}, issn = {0040-5752}, journal = {THEORETICAL AND APPLIED GENETICS}, keywords = {IFZ imported}, number = 7, pages = {917-931}, timestamp = {2008-06-04T14:34:51.000+0200}, title = {Genetic diversity and population differentiation of traditional fonio millet (Digitaria spp.) landraces from different agro-ecological zones of West Africa}, volume = 115, year = 2007 } @article{ISI:000251205700008, abstract = {Resynthesized (RS) forms of rapeseed (Brassica napus L.; genome AACC}, added-at = {2008-06-04T14:34:51.000+0200}, author = {Rygulla, W. and Friedt, W. and Seyis, F. and Luehs, W. and Eynck, C. and von Tiedemann, A. and Snowdon, R. J.}, biburl = {http://www.bibsonomy.org/bibtex/2d90451043304c4003b79a29f33446b98/ipzgiessen}, interhash = {9d8a7b8070ba345bd563ab14a87b13f1}, intrahash = {d90451043304c4003b79a29f33446b98}, issn = {0179-9541}, journal = {PLANT BREEDING}, keywords = {B._oleracea B._rapa Brassica_napus IFZ Verticillium_longisporum erucic_acid resistance resynthesized_rapeseed}, number = 6, pages = {596-602}, timestamp = {2008-06-04T14:34:51.000+0200}, title = {Combination of resistance to Verticillium longisporum from zero erucic acid Brassica oleracea and oilseed Brassica rapa genotypes in resynthesized rapeseed (Brassica napus) lines}, volume = 126, year = 2007 } @article{ISI:000250326500002, abstract = {Verticillium wilt caused by the vascular fungal pathogen Verticillium longisporum is one of the most important pathogens of oilseed rape (Brassica napus sp. oleifera) in northern Europe. Because production of this major oilseed crop is expanding rapidly and no approved fungicides are available for V longisporum, long-term control of the disease can only be achieved with cultivars carrying effective quantitative resistance. However, very little resistance to V longisporum is available within the gene pool of oilseed rape, meaning that interspecific gene transfer from related species is the only possibility for broadening levels of resistance in current varieties. The amphidiploid species B. napus can be resyn-thesized by crossing the two progenitor species Brassica oleracea and Brassica rapa, hence resistant accessions of these two diploid species can be used as resistance donors. In this study a total of 43 potential B. rapa and B. oleracea resistance donors were tested with regard to their reaction to a mixture of two aggressive V longisporum isolates, and resistances from diverse lines were combined by embryo rescue-assisted interspecific hybridization in resynthesized rapeseed lines. Progenies from crosses of the two B. rapa gene bank accessions 13444 and 56515 to the B. oleracea gene bank accessions BRA 1008, CGN 14044, 8207, BRA 1398, and 7518 showed a broad spectrum of resistance in pathogenicity tests. Of 45 tested resynthesized lines, 41 lines exhibited a significantly higher level of resistance than the moderately V longisporum-tolerant oilseed rape cultivar Express. These lines represent a promising basis for the combination of different resistance resources in new varieties.}, added-at = {2008-06-04T14:34:51.000+0200}, author = {Rygulla, W. and Snowdon, R. J. and Eynck, C. and Koopmann, B. and von Tiedemann, A. and Luehs, W. and Friedt, W.}, biburl = {http://www.bibsonomy.org/bibtex/29e4259e779d518dcda2cb73e08538399/ipzgiessen}, interhash = {1395c6353eb64f4227946400e4319fd7}, intrahash = {9e4259e779d518dcda2cb73e08538399}, issn = {0031-949X}, journal = {PHYTOPATHOLOGY}, keywords = {IFZ imported}, number = 11, pages = {1391-1396}, timestamp = {2008-06-04T14:34:51.000+0200}, title = {Broadening the genetic basis of Verticillium longisporum resistance in Brassica napus by interspecific hybridization}, volume = 97, year = 2007 } @article{ISI:000251205700006, abstract = {Generation of novel genetic diversity for maximization of heterosis in hybrid production is a significant goal in winter oilseed rape breeding. Here, we demonstrate that doubled haploid (DH) production using microspore cultivation can simultaneously introgress favourable alleles for double-low seed quality (low erucic acid and low-glucosinolate content) into a genetically diverse Brassica napus genetic background. The DH lines were derived from a cross between a double-low quality winter rapeseed variety and a genetically diverse semisynthetic B. napus line with high erucic acid and high glucosinolates (+ + quality). Twenty-three low-glucosinolate lines were identified with a genome component of 50-67% derived from the + + parent. Four of these lines, with a genome component of 50-55% derived from the + + parent, also contained low erucic acid. Heterosis for seed yield was confirmed in test-crosses using these genetically diverse lines as pollinator. The results demonstrate the potential of marker-assisted identification of novel genetic pools for breeding of double-low quality winter oilseed rape hybrids.}, added-at = {2008-06-04T14:34:51.000+0200}, author = {Basunanda, P. and Spiller, T. H. and Hasan, M. and Gehringer, A. and Schondelmaier, J. and Luehs, W. and Friedt, W. and Snowdon, R. J.}, biburl = {http://www.bibsonomy.org/bibtex/23646944cbc41937e67ba28bf343491af/ipzgiessen}, interhash = {180457704d5ea14faf9892957f4ce3d6}, intrahash = {3646944cbc41937e67ba28bf343491af}, issn = {0179-9541}, journal = {PLANT BREEDING}, keywords = {Brassica_napus IFZ Oilseed_rape QTL double-low_quality heterosis locus quantitative_trait}, number = 6, pages = {581-587}, timestamp = {2008-06-04T14:34:51.000+0200}, title = {Marker-assisted increase of genetic diversity in a double-low seed quality winter oilseed rape genetic background}, volume = 126, year = 2007 } @article{ISI:000250580500007, abstract = {Barley yellow mosaic virus disease caused by different strains of BaYMV and BaMMV is a major threat to winter barley cultivation in Europe. Different resistance genes against these viruses have been mapped and suitable PCR-based markers have been developed. In this respect doubled haploid (DH) populations proved to be advantageous as they facilitate a repeated test for resistance against all agents of the barley yellow mosaic virus complex and besides this, dominant marker systems are as informative as co-dominant ones in DHs due to the lack of heterozygous genotypes. Using DH populations resistance genes rym4, rym5, rym11, rym13, rym15 and the BaYMV/BaYMV-2 resistance of the barley cultivar �Chikurin Ibaraki 1� have been mapped. DHs are also well suited to pyramiding resistance genes against BaMMV and BaYMV. Since homozygous recessive genotypes are more frequent in DHs than in segregating F-2 populations, DHs can be efficiently used to create broad-spectrum resistance and to extend the usability of partly overcome resistance genes. Results from employing two different strategies for pyramiding, based on one and two DH-steps, respectively, combining three recessive resistance genes, i.e. rym4/rym5, rym9 and rym11, are presented. The faster strategy based on one haploidy step resulted in the identification of all three and two-way combinations of the respective resistance genes.}, added-at = {2008-06-04T14:34:51.000+0200}, author = {Werner, Kay and Friedt, Wolfgang and Ordon, Frank}, biburl = {http://www.bibsonomy.org/bibtex/2989387bba9e15d958b257418fad44fa7/ipzgiessen}, interhash = {fd68065e899b416d46f15a22fd01525e}, intrahash = {989387bba9e15d958b257418fad44fa7}, issn = {0014-2336}, journal = {EUPHYTICA}, keywords = {BaMMV BaYMV IFZ barley_(Hordeum_vulgare_L) doubled_haploids_(DHs) molecular_markers pyramiding resistance}, number = 3, pages = {323-329}, timestamp = {2008-06-04T14:34:51.000+0200}, title = {Localisation and combination of resistance genes against soil-borne viruses of barley (BaMMV, BaYMV) using doubled haploids and molecular markers}, volume = 158, year = 2007 } @article{ISI:000252608200007, abstract = {Winter oilseed rape (Brassica napus L.) is the most important oil crop in Europe. Due to a continually increasing demand for rapeseed oil for food and non-food uses, the production of hybrid cultivars with higher seed and oil yields has become increasingly important in recent years. However, the systematic use of heterosis for hybrid breeding in oilseed rape is limited by the relatively narrow genetic basis of adapted germplasm, which can impede the generation of distinct heterotic pools. In the present study experimental hybrids were developed from a population of 190 DH lines derived from a cross between an elite, double-low seed quality (zero erucic acid, low glucosinolate content) winter oilseed rape variety and a semi-synthetic line derived from a genetically diverse resynthesised rapeseed line with high erucic acid and glucosinolate contents. The DH lines were crossed with a male sterile tester and the resulting test hybrids were examined for yield performance at two locations in Hesse, Germany, that exhibit extreme differences in climatic conditions and soil characteristics. Mid-parent heterosis for seed yield was determined at both the agronomically optimal location Rauischholzhausen and the marginal site Niederhorlen. A value of up to 43% mid-parent heterosis for seed yield could be observed among selected test hybrids compared to that of their parental DH lines. The heterosis level for yield was particularly high at the nutrient-poor site, where the best test hybrids showed significantly higher yields than elite open-pollinating and hybrid varieties. This demonstrates the suitability and adaptability of highly heterotic rapeseed hybrids on marginal locations and suggests the existence of a strong heterotic effect on nutrient uptake efficiency.}, added-at = {2008-06-04T14:34:51.000+0200}, author = {Gehringer, Anke and Snowdon, Rod and Spiller, Tobias and Basunanda, Panjisakti and Friedt, Wolfgang}, biburl = {http://www.bibsonomy.org/bibtex/26cd032d536b46854bb346d3978b48dff/ipzgiessen}, interhash = {f128259c52624bce37fa6e6d185cdaf9}, intrahash = {6cd032d536b46854bb346d3978b48dff}, issn = {1344-7610}, journal = {BREEDING SCIENCE}, keywords = {IFZ Oilseed_rape heterosis hybrids marginal_conditions}, number = 4, pages = {315-320}, timestamp = {2008-06-04T14:34:51.000+0200}, title = {New oilseed rape (Brassica napus) hybrids with high levels of heterosis for seed yield under nutrient-poor conditions}, volume = 57, year = 2007 } @article{ISI:000249460600014, abstract = {Nuclear DNA amounts of 118 cultivated fonio accessions representing 94 landraces collected from the major growing areas of West-Africa (Benin, Burkina Faso, Guinea, Mali and Togo) and eight accessions of four wild relatives were investigated by Laser flow cytometry. In cultivated species, average 2C-values ranged from 1.848 +/- 0.031 pg for Digitaria iburua to 1.956 +/- 0.004 pg for D. exilis. In D. exilis landraces the}, added-at = {2008-06-04T14:34:51.000+0200}, author = {Adoukonou-Sagbadja, H. and Schubert, V. and Dansi, A. and Jovtchev, G. and Meister, A. and Pistrick, K. and Akpagana, K. and Friedt, W.}, biburl = {http://www.bibsonomy.org/bibtex/2a4fc7805481e5b2b87e577f2e0bf2224/ipzgiessen}, interhash = {34563de666627601549ab0e8df7b7a3f}, intrahash = {a4fc7805481e5b2b87e577f2e0bf2224}, issn = {0378-2697}, journal = {PLANT SYSTEMATICS AND EVOLUTION}, keywords = {2C-values Digitaria_spp. Fonio IFZ West-Africa chromosome_number flow_cytometry genome_size}, number = {1-4}, pages = {163-176}, timestamp = {2008-06-04T14:34:51.000+0200}, title = {Flow cytometric analysis reveals different nuclear DNA contents in cultivated Fonio (Digitaria spp.) and some wild relatives from West-Africa}, volume = 267, year = 2007 } @article{ISI:000252447600018, abstract = {Powdery mildew is an important pathogen of barley in many barley-growing areas. To identify molecular markers for the resistance gene Mlg located on chromosome 4H, a cDNA-AFLP approach was carried out on near-isogenic lines. Based on the identification of a differentiating fragment of 37 bp, which turned out to be part of a nucleoside diphosphate kinase as estimated by BLASTN, a CAPS marker has been developed, which co-segregated with Mlg in a population of 738 F-2-plants. Due to its co-dominant inheritance, clear banding pattern and close linkage this marker is well suited for marker-based selection procedures.}, added-at = {2008-06-04T14:34:51.000+0200}, author = {Korell, M. and Eschholz, T. W. and Eckey, C. and Biedenkopf, D. and Kogel, Marc K. H. and Friedt, W. and Ordon, F.}, biburl = {http://www.bibsonomy.org/bibtex/2d52cc20d6412bd122b3666be880a258f/ipzgiessen}, interhash = {a1cd61dc9e5e8301280e743f93ae2697}, intrahash = {d52cc20d6412bd122b3666be880a258f}, issn = {0179-9541}, journal = {PLANT BREEDING}, keywords = {Blumeria_graminis CAPS-marker Hordeum_vulgare IFZ Mlg cDNA-AFLP}, number = 1, pages = {102-104}, timestamp = {2008-06-04T14:34:51.000+0200}, title = {Development of a cDNA-AFLP derived CAPS marker co-segregating with the powdery mildew resistance gene Mlg in barley}, volume = 127, year = 2008 } @article{ISI:000252612300009, abstract = {Verticillium longisporum is one of the major pathogens of oilseed rape}, added-at = {2008-06-04T14:34:51.000+0200}, author = {Rygulla, W. and Snowdon, R. J. and Friedt, W. and Happstadius, I. and Cheung, W. Y. and Chen, D.}, biburl = {http://www.bibsonomy.org/bibtex/21cf4cb19ae74356eaeec44702a6fa96e/ipzgiessen}, interhash = {80e4a90a6496385393f72da56d9f57b3}, intrahash = {1cf4cb19ae74356eaeec44702a6fa96e}, issn = {0031-949X}, journal = {PHYTOPATHOLOGY}, keywords = {imported}, number = 2, pages = {215-221}, timestamp = {2008-06-04T14:34:51.000+0200}, title = {Identification of quantitative trait loci for resistance against Verticillium longisporum in oilseed rape (Brassica napus)}, volume = 98, year = 2008 }