@article{stellwagen01a, title = {Measuring the translational diffusion coefficients of small DNA molecules by capillary electrophoresis}, author = {N. C. Stellwagen and S. Magnusdottir and C. Gelfi and P. G. Righetti}, journal = {Biopolymers}, month = {April}, number = 4, pages = {390--397}, volume = 58, year = 2001, timestamp = {2007.06.11}, de = {DNA; translational diffusion coefficients; capillary electrophoresis}, sn = {0006-3525}, owner = {grass}, ut = {ISI:000167129100004}, abstract = {The apparent translational diffusion coefficients of four 20 base pair (bp) DNA oligonucleotides with different sequences have been measured by capillary electrophoresis. using the stopped migration method The diffusion coefficients of the four oligomers were equal within experimental error, and averaged (120 +/- 10) x 10(-8) cm(2) s(-1) in 40 mM Tris-acetate-EDTA buffer at 25 degreesC. Since this value is nearly identical to the translational diffusion coefficient determined for a different 20-bp oligomer using other methods, the stopped migration method can accurately measure the diffusion coefficients of small DNA oligomers. The apparent diffusion coefficient of a 118-bp DNA restriction fragment was also measured by the stopped migration method. However. the observed value was similar to 25% larger than expected from other measurements, possibly because the diffusion coefficients of larger DNA molecules are somewhat dependent on the ionic strength of the solution. (C) 2001 John Wiley & Sons, Inc. Biopolymers 58: 390-397, 2001.}, biburl = {http://www.bibsonomy.org/bibtex/2709c3dfd280810ec232fefd13c170c47/kaigrass}, keywords = {FREE MODEL; DISPERSION; SALT SOLUTION ZONE LIGHT-SCATTERING; PROTEINS; GEL; MOBILITY; PEPTIDES; DYNAMIC ELECTROPHORESIS; FRAGMENTS;} } @article{slater03a, title = {The theory of {DNA} separation by capillary electrophoresis}, author = {G. W. Slater and M. Kenward and L. C. McCormick and M. G. Gauthier}, journal = {Current Opinion in Biotechnology}, month = {February}, number = 1, pages = {58--64}, volume = 14, year = 2003, timestamp = {2006.11.07}, pdf = {slater03a.pdf}, owner = {grass}, comment = {outstanding}, abstract = {The Human Genome has been sequenced in large part owing to the invention of capillary electrophoresis. Although this technology has matured enough to allow such amazing achievements, the physical mechanisms at play during separation have yet to be completely understood and optimized. Recently, new separation regimes and new physical mechanisms have been investigated. The use of free-flow electrophoresis and new modes of pulsed-field electrophoresis have been suggested, while we have observed a shift towards single nucleotide polymorphism analysis and microchip technologies. A strong theoretical basis remains essential for the efficient development of new methods.}, biburl = {http://www.bibsonomy.org/bibtex/216793421ab53a9776c09e0739630d8be/kaigrass}, keywords = {MODEL; 3-DIMENSIONAL GELS; OGSTON MOLECULES; POLYMER-SOLUTIONS; REPTATION DIFFUSION; GEL-ELECTROPHORESIS; SINGLE-STRANDED-DNA; SOLVABLE MOBILITY; ENTANGLED review, experiment, theory, OBSTRUCTION-SCALING} } @article{slater02b, title = {Theory of {DNA} electrophoresis (similar to 1999-2002(1)/(2))}, author = {G. W. Slater and S. Guillouzic and M. G. Gauthier and J. F. Mercier and M. Kenward and L. C. McCormick and F. Tessier}, journal = {Electrophoresis}, month = {November}, number = {22-23}, pages = {3791--3816}, volume = 23, year = 2002, timestamp = {2006.11.07}, pdf = {slater02b.pdf}, owner = {grass}, comment = {outstanding}, abstract = {Over the last two decades, the introduction of new methods such as pulsed-field gel electrophoresis and capillary array electrophoresis has made it possible to map and sequence entire genomes, including our own. The development-of these experimental methods has been helped by the progress of theoretical and computational sciences, and the interactions between these three modi operandi of modern science are still pushing the limits of our technologies. We now see a clear trend towards proteomics and microfluidic (even nanofluidic!) devices. In this review, we take a look at the progress of the field over the last 3 years using the glasses of the theoretical scientist and focusing mostly on new ideas and concepts. About a dozen different subfields are discussed and reviewed. We conclude by giving a commented list of some of the best review articles published over the last 2-3 years.}, biburl = {http://www.bibsonomy.org/bibtex/24fbe0ee292432cb0e68900b2c626d985/kaigrass}, keywords = {MODEL; METHOD; MOLECULAR BOND CAPILLARY-ELECTROPHORESIS; MONTE-CARLO-SIMULATION SOLUTION OGSTON POLYMER-SOLUTIONS; FLUCTUATION SINGLE-STRANDED-DNA; ULTRADILUTE DIFFUSION-COEFFICIENTS; COMPOSITE PERIODIC EXACT OBJECTS; SOLVABLE BOUNDARY-CONDITIONS; review, experiment, theory, OBSTRUCTION-SCALING} } @article{slater00a, title = {Theory of {DNA} electrophoresis: {A} look at some current challenges}, author = {G. W. Slater and C. Desrulsseaux and S. J. Hubert and J. F. Mercier and J. Labrie and J. Boileau and F. Tessier and M. P. Pepin}, journal = {Electrophoresis}, month = {December}, number = 18, pages = {3873--3887}, volume = 21, year = 2000, timestamp = {2006.11.07}, pdf = {slater00a.pdf}, owner = {grass}, comment = {outstanding}, abstract = {Although electrophoresis is one of the basic methods of the modern molecular biology laboratory, new ideas are being suggested at an accelerated rate, in large part because of the pressing demands of the biomedical community. Although we now have, at least for some methods, a fairly good theoretical understanding of the physical mechanisms that lead to the observed peak spacings, widths and shapes, this knowledge is often too qualitative to be used to guide further technical developments and improvements. In this article, we review some selected elements of the current state of our theoretical ignorance, focusing mostly on DNA electrophoresis, and we offer several suggestions for further theoretical investigations.}, biburl = {http://www.bibsonomy.org/bibtex/20649bd7b2ed120342a4698f20bbd7d05/kaigrass}, keywords = {MODEL; AGAROSE CAPILLARY-ELECTROPHORESIS; OGSTON POLYMER-SOLUTIONS; GEL-ELECTROPHORESIS; SINGLE-STRANDED-DNA; DNA; ULTRADILUTE POLYMER; END-LABELED PULSED-FIELD TRAPPING LOW-VISCOSITY SOLVABLE GELS ELECTROPHORESIS; review, experiment, theory,} } @article{jendrejack02a, title = {Stochastic simulations of DNA in flow: Dynamics and the effects of hydrodynamic interactions}, author = {R. M. Jendrejack and J. J. de Pablo and M. D. Graham}, journal = {Journal Of Chemical Physics}, month = {May}, number = 17, pages = {7752--7759}, volume = 116, year = 2002, timestamp = {2007.06.11}, sn = {0021-9606}, owner = {grass}, ut = {ISI:000175068800046}, abstract = {We present a fully parametrized bead-spring chain model for stained lambda-phage DNA. The model accounts for the finite extensibility of the molecule, excluded volume effects, and fluctuating hydrodynamic interactions (HI). Parameters are determined from equilibrium experimental data for 21 mum stained lambda-phage DNA, and are shown to quantitatively predict the non-equilibrium behavior of the molecule. The model is then used to predict the equilibrium and nonequilibrium behavior of DNA molecules up to 126 mum. In particular, the HI model gives results that are in quantitative agreement with experimental diffusivity data over a wide range of molecular weights. When the bead friction coefficient is fit to the experimental relaxation time at a particular molecular weight, the stretch in shear and extensional flows is adequately predicted by either a free-draining or HI model at that molecular weight, although the fitted bead friction coefficients for the two models differ significantly. In shear flow, we find two regimes at high shear rate (gamma) that follow different scaling behavior. In the first, the viscosity and first normal stress coefficient scale roughly as gamma(-6/11) and gamma(-14/11), respectively. At higher shear rates, these become gamma(-2/3) and gamma(-4/3). These regimes are found for both free-draining and HI models and can be understood based on scaling arguments for the diffusion of chain ends. (C) 2002 American Institute of Physics.}, biburl = {http://www.bibsonomy.org/bibtex/27d3b84c93752a744c1cc952a5b3671bd/kaigrass}, keywords = {MODEL; POLYMERS; FLEXIBLE MOTION; FLOW; MOLECULES; SHEAR-FLOW; CHAIN; FIELD ELASTICITY; DYNAMICS; ELONGATIONAL SINGLE-POLYMER} }