@article{glaeser_protein_2007,
        title = {Protein synthesis patterns reveal a complex regulatory response to singlet oxygen in Rhodobacter},
        author = {Jens Glaeser and Monica Zobawa and Friedrich Lottspeich and Gabriele Klug},
        journal = {Journal of proteome research},
        month = {July},
        note = {PMID: 17536848},
        pages = {2460-71},
        volume = 6,
        year = 2007,
        issn = {15353893},
	abstract = {Singlet oxygen (1O2) is a stress factor and signal in the facultative phototrophic bacterium Rhodobacter sphaeroides. In vivo protein labeling with L-[35S]-methionine and analysis by two-dimensional gel electrophoresis revealed that the synthesis of 61 proteins was changed in response to 1O2. After 1O2 treatment, protein synthesis patterns were distinct from those after H2O2 treatment but similar to those after high light exposure. This indicates regulatory mechanisms selective for different reactive oxygen species (ROS) and a response to light partly mediated by 1O2. Analysis of mutant strains support that the response to 1O2 is regulated mainly by rpoE (sigma E), but also a modulation of the sigma E dependent response by other factors and the existence of sigma E independent responses. The involvement of the RNA chaperon Hfq in the 1O2 response implies a role of small regulatory RNAs.},
	biburl = {http://www.bibsonomy.org/bibtex/27ffd100e93e552721654848a86efd795/mikromolbio},
	keywords = {Reactive_Oxygen_Species Bacterial_Proteins Messenger Singlet_Oxygen Divalent Gel Rhodobacter_sphaeroides Transcription_Factors Sigma_Factor Host_Factor_1_Protein Light Mutation Gene_Expression_Regulation Electrophoresis Two-Dimensional RNA Bacterial Proteome Hydrogen_Peroxide IFZ Cations Protein_Biosynthesis Catalase}
}

@article{zeller_regulation_2007,
        title = {Regulation of hydrogen peroxide-dependent gene expression in Rhodobacter sphaeroides: regulatory functions of OxyR},
        author = {Tanja Zeller and Mobarak A Mraheil and Oleg V Moskvin and Kuanyu Li and Mark Gomelsky and Gabriele Klug},
        journal = {Journal of bacteriology},
        month = {May},
        note = {PMID: 17351037},
        pages = {3784-92},
        volume = 189,
        year = 2007,
        issn = {00219193},
	abstract = {Genome-wide transcriptome profiling was used to reveal hydrogen peroxide (H(2)O(2))-dependent regulatory mechanisms in the facultatively photosynthetic bacterium Rhodobacter sphaeroides. In this study we focused on the role of the OxyR protein, a known regulator of the H(2)O(2) response in bacteria. The transcriptome profiles of R. sphaeroides wild-type and oxyR mutant strains that were exposed to 1 mM H(2)O(2) for 7 min or were not exposed to H(2)O(2) were analyzed. Three classes of OxyR-dependent genes were identified based on their expression patterns in the wild type of oxyR mutant strains with differing predicted roles of oxidized and reduced OxyR as activators of transcription. DNA binding studies revealed that OxyR binds upstream of class I genes, which are induced by H(2)O(2) and exhibit similar basal levels of expression in the wild-type and oxyR mutant strains. The effect of OxyR on class II genes, which are also induced by H(2)O(2) but exhibit significantly lower basal levels of expression in the wild-type strain than in the mutant, is indirect. Interestingly, reduced OxyR also activates expression of few genes (class III). The role of reduced OxyR as an activator is shown for the first time. Our data reveal that the OxyR-mediated response is fast and transient. In addition, we found that additional regulatory pathways are involved in the H(2)O(2) response.},
	biburl = {http://www.bibsonomy.org/bibtex/28f70cb140c45721ef0acab94f03009d2/mikromolbio},
	keywords = {Regulon Transcription_Factors Mutation Gene_Expression_Regulation Rhodobacter_sphaeroides Bacterial Oxidants Genetic Molecular_Sequence_Data Hydrogen_Peroxide Base_Sequence Consensus_Sequence Transcription IFZ}
}