@article{geueke_description_2007, title = {Description of Sphingosinicella xenopeptidilytica sp. nov., a beta-peptide-degrading species, and emended descriptions of the genus Sphingosinicella and the species Sphingosinicella microcystinivorans}, author = {Birgit Geueke and Hans-Jürgen Busse and Thomas Fleischmann and Peter Kämpfer and Hans-Peter E Kohler}, journal = {International journal of systematic and evolutionary microbiology}, note = {PMID: 17220451}, pages = {107-13}, volume = 57, year = 2007, issn = {14665026}, abstract = {A Gram-negative, rod-shaped bacterium, strain 3-2W4(T), was isolated from the aeration tank of a wastewater treatment plant in Zurich and was found to have the exceptional capacity to degrade synthetic beta-peptides. 16S rRNA gene sequence analysis showed that strain 3-2W4(T) is closely related to Sphingosinicella microcystinivorans Y2(T), but DNA-DNA hybridization experiments between these two strains revealed that they belong to two different species. The two strains displayed different fingerprints after PCR analysis using the repetitive primers BOX, ERIC and REP. Strain 3-2W4(T) did not degrade microcystin, which is a characteristic trait of Sphingosinicella microcystinivorans Y2(T). Like Sphingosinicella microcystinivorans Y2(T), strain 3-2W4(T) had the following characteristics: fatty acids comprising mainly C(18 : 1)omega7c, summed feature 3 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH) and C(16 : 0), the presence of ubiquinone Q-10 and sym-homospermidine as the predominant polyamine compound. The polar lipid profiles of the two strains were almost identical, consisting of phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol and sphingoglycolipid. Strain 3-2W4(T) and Sphingosinicella microcystinivorans Y2(T) utilized the beta-peptides H-betahVal-betahAla-betahLeu-OH and H-betahAla-betahLeu-OH as sole carbon and energy sources and shared beta-peptidyl aminopeptidase activity in common, which distinguishes them from Sphingomonas and Sphingopyxis type strains. On the basis of these results, strain 3-2W4(T) represents a novel species of the genus Sphingosinicella, for which the name Sphingosinicella xenopeptidilytica sp. nov. is proposed. The type strain is 3-2W4(T) (=DSM 17130(T)=CCUG 52537(T)). The descriptions of the genus Sphingosinicella and the species Sphingosinicella microcystinivorans are emended.}, biburl = {http://www.bibsonomy.org/bibtex/22d64ff0fa9abfdaa4aa77811fae436b4/recymikrobio}, keywords = {Ribosomal Phylogeny Species_Specificity Microcystins Molecular_Sequence_Data Waste_Disposal Aminopeptidases Nucleic_Acid_Hybridization Switzerland Bacterial_Typing_Techniques Sphingomonadaceae Sequence_Analysis rRNA Genes RNA 16S IFZ Water_Microbiology DNA Peptides Bacterial Fluid} } @article{wittich_sphingobium_2007, title = {Sphingobium aromaticiconvertens sp. nov., a xenobiotic-compound-degrading bacterium from polluted river sediment}, author = {Rolf-Michael Wittich and Hans-Jürgen Busse and Peter Kämpfer and Marja Tiirola and Monika Wieser and Alexandre J Macedo and Wolf-Rainer Abraham}, journal = {International journal of systematic and evolutionary microbiology}, month = {February}, note = {PMID: 17267969}, pages = {306-10}, volume = 57, year = 2007, issn = {14665026}, abstract = {A bacterial strain capable of degrading some monochlorinated dibenzofurans, designated RW16T, was isolated from aerobic River Elbe sediments. The strain was characterized based on 16S rRNA gene sequence analysis, DNA G+C content, physiological characteristics, polyamines, ubiquinone and polar lipid pattern and fatty acid composition. This analysis revealed that strain RW16T represents a novel species of the genus Sphingobium. The DNA G+C content of strain RW16T, 60.7 mol\%, is the lowest yet reported for the genus. 16S rRNA gene sequence analysis placed strain RW16T as an outlier in the genus Sphingobium. The name Sphingobium aromaticiconvertens sp. nov. is proposed for this dibenzofuran-mineralizing organism, with type strain RW16T (=DSM 12677T=CIP 109198T).}, biburl = {http://www.bibsonomy.org/bibtex/20d89a15a716dc6027c7034795d176a28/recymikrobio}, keywords = {Polyamines Chemical Bacterial_Typing_Techniques Quinones Sequence_Analysis Sphingomonadaceae rRNA Genes 16S IFZ Bacterial Phospholipids Phylogeny Ribosomal Rivers Geologic_Sediments Carbohydrate_Metabolism Molecular_Sequence_Data Fatty_Acids Sequence_Homology Benzofurans Base_Composition RNA DNA Germany Water_Pollution Nucleic_Acid} }