%0 Journal Article %1 BIT:BIT21386 %A Zhang, Yi-Heng Percival %A Ding, Shi-You %A Mielenz, Jonathan R. %A Cui, Jing-Biao %A Elander, Richard T. %A Laser, Mark %A Himmel, Michael E. %A McMillan, James R. %A Lynd, Lee R. %D 2007 %I Wiley Subscription Services, Inc., A Wiley Company %J Biotechnology and Bioengineering %K lignocellulose %N 2 %P 214--223 %R 10.1002/bit.21386 %T Fractionating recalcitrant lignocellulose at modest reaction conditions %U http://dx.doi.org/10.1002/bit.21386 %V 97 %X Effectively releasing the locked polysaccharides from recalcitrant lignocellulose to fermentable sugars is among the greatest technical and economic barriers to the realization of lignocellulose biorefineries because leading lignocellulose pre-treatment technologies suffer from low sugar yields, and/or severe reaction conditions, and/or high cellulase use, narrow substrate applicability, and high capital investment, etc. A new lignocellulose pre-treatment featuring modest reaction conditions (50°C and atmospheric pressure) was demonstrated to fractionate lignocellulose to amorphous cellulose, hemicellulose, lignin, and acetic acid by using a non-volatile cellulose solvent (concentrated phosphoric acid), a highly volatile organic solvent (acetone), and water. The highest sugar yields after enzymatic hydrolysis were attributed to no sugar degradation during the fractionation and the highest enzymatic cellulose digestibility (∼97% in 24 h) during the hydrolysis step at the enzyme loading of 15 filter paper units of cellulase and 60 IU of beta-glucosidase per gram of glucan. Isolation of high-value lignocellulose components (lignin, acetic acid, and hemicellulose) would greatly increase potential revenues of a lignocellulose biorefinery. Biotechnol. Bioeng. 2007;97: 214–223. © 2007 Wiley Periodicals, Inc.

@article{BIT:BIT21386, abstract = {Effectively releasing the locked polysaccharides from recalcitrant lignocellulose to fermentable sugars is among the greatest technical and economic barriers to the realization of lignocellulose biorefineries because leading lignocellulose pre-treatment technologies suffer from low sugar yields, and/or severe reaction conditions, and/or high cellulase use, narrow substrate applicability, and high capital investment, etc. A new lignocellulose pre-treatment featuring modest reaction conditions (50°C and atmospheric pressure) was demonstrated to fractionate lignocellulose to amorphous cellulose, hemicellulose, lignin, and acetic acid by using a non-volatile cellulose solvent (concentrated phosphoric acid), a highly volatile organic solvent (acetone), and water. The highest sugar yields after enzymatic hydrolysis were attributed to no sugar degradation during the fractionation and the highest enzymatic cellulose digestibility (∼97% in 24 h) during the hydrolysis step at the enzyme loading of 15 filter paper units of cellulase and 60 IU of beta-glucosidase per gram of glucan. Isolation of high-value lignocellulose components (lignin, acetic acid, and hemicellulose) would greatly increase potential revenues of a lignocellulose biorefinery. Biotechnol. Bioeng. 2007;97: 214–223. © 2007 Wiley Periodicals, Inc.}, added-at = {2014-06-26T14:01:11.000+0200}, author = {Zhang, Yi-Heng Percival and Ding, Shi-You and Mielenz, Jonathan R. and Cui, Jing-Biao and Elander, Richard T. and Laser, Mark and Himmel, Michael E. and McMillan, James R. and Lynd, Lee R.}, biburl = {https://www.bibsonomy.org/bibtex/2fd44de2a15ab1fc8f7d86c176f8f4134/docal}, description = {Fractionating recalcitrant lignocellulose at modest reaction conditions - Zhang - 2007 - Biotechnology and Bioengineering - Wiley Online Library}, doi = {10.1002/bit.21386}, interhash = {005fc14e2a4ff1b6455269504520cf19}, intrahash = {fd44de2a15ab1fc8f7d86c176f8f4134}, issn = {1097-0290}, journal = {Biotechnology and Bioengineering}, keywords = {lignocellulose}, number = 2, pages = {214--223}, publisher = {Wiley Subscription Services, Inc., A Wiley Company}, timestamp = {2014-06-26T14:01:11.000+0200}, title = {Fractionating recalcitrant lignocellulose at modest reaction conditions}, url = {http://dx.doi.org/10.1002/bit.21386}, volume = 97, year = 2007 }