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Preparation and characterisation of chitosans with oligosaccharide branches
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Carbohydr. Res. 337 (24): 2455 -- 2462 (November 2002)

The trimer 2-acetamido-2-deoxy-D-glucopyranosyl-beta-(1 --> 4)-2-acetamido-2-deoxy-D-glucopyranosyl-beta-(1 --> 4)-2,5-anhydro-D-mannofuranose (A-A-M) was reductively N-alkylated onto a fully de-N-acetylated chitosan (F-A < 0.001, DPn = 25) to obtain branched chitosans with degree of substitution (DS) of 0.070, 0.23 and 0.40, as determined by H-1 NMR spectroscopy. The apparent pK(a) values of the primary and secondary amines of the chitosans substituted with the trimer A-A-M were determined by monitoring the chemical shift of the H-2 of GlcN, and were determined as 6.5-6.9 for the primary (unsubstituted) amines and as 5.0-5.2 for the secondary (substituted) amines. The intrinsic pK(a) values (pK(int)) were found to be 7.3-7.4 for the substituted and 8.7 for the unsubstituted amines. The chitosan branched with A-A-M (DS 0.40) was found to be soluble in aqueous solution over the entire pH range. SEC-MALLS (size-exclusion chromatography with a multi-angle laser light scattering detector) further showed that addition of branches did not affect the molar hydrodynamic volume of the chitosan. (C) 2002 Elsevier Science Ltd. All rights reserved.
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