%0 Journal Article %1 Selz2007 %A Selz, Y. %A Braasch, I. %A Hoffmann, C. %A Schmidt, C. %A Schultheis, C. %A Schartl, M. %A Volff, J. N. %D 2007 %J Gene %K *Evolution, 1/chemistry/genetics/*metabolism Acid Amino Analysis, Animals Cell Cloning, Cyprinodontiformes/*genetics Data Dosage Experimental Gene Genetic Homology, Line Melanocortin, Melanocortins/agonists/genetics/metabolism Melanoma, Melanophores/metabolism Models, Molecular Oryzias/*genetics Phylogeny Protein Sequence Structure, Substitution Tertiary Type Zebrafish/*genetics Receptor %N 1-2 %P 114-22 %T Evolution of melanocortin receptors in teleost fish: the melanocortin type 1 receptor %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17707598 %V 401 %X The melanocortin type 1 receptor (Mc1r) belongs to a family of G-protein-coupled receptors involved in various physiological processes in vertebrates. Melanocortins, the Mcr natural agonists, are pituitary peptide hormones including adrenocorticotropin and melanocyte-stimulating hormones. In mammals and birds, Mc1r is involved in pigmentation and expressed in melanocytes and melanoma. Activation of Mc1r leads to eumelanin production as well as to proliferation and survival of melanocytes in the epidermis. Here we report the molecular and evolutionary analysis of mc1r from three major fish models, the zebrafish Danio rerio, the medaka Oryzias latipes and the platyfish Xiphophorus maculatus. In contrast to some other melanocortin receptor genes, mc1r has been conserved as a single copy gene in divergent fish species. Its expression was detected in all organs tested in platyfish and medaka but was restricted to eyes, skin, brain and testis in zebrafish, this possibly reflecting differences in the distribution of extracutaneous melanophores. The mc1r gene was found to be expressed during embryogenesis, as well as in Xiphophorus hybrid melanoma, similar to human tumours. Protein sequence comparison between fish and mammalian Mc1r revealed a remarkable concordance between evolutionary and functional analyses for the identification of residues and regions critical for receptor function.

@article{Selz2007, abstract = {The melanocortin type 1 receptor (Mc1r) belongs to a family of G-protein-coupled receptors involved in various physiological processes in vertebrates. Melanocortins, the Mcr natural agonists, are pituitary peptide hormones including adrenocorticotropin and melanocyte-stimulating hormones. In mammals and birds, Mc1r is involved in pigmentation and expressed in melanocytes and melanoma. Activation of Mc1r leads to eumelanin production as well as to proliferation and survival of melanocytes in the epidermis. Here we report the molecular and evolutionary analysis of mc1r from three major fish models, the zebrafish Danio rerio, the medaka Oryzias latipes and the platyfish Xiphophorus maculatus. In contrast to some other melanocortin receptor genes, mc1r has been conserved as a single copy gene in divergent fish species. Its expression was detected in all organs tested in platyfish and medaka but was restricted to eyes, skin, brain and testis in zebrafish, this possibly reflecting differences in the distribution of extracutaneous melanophores. The mc1r gene was found to be expressed during embryogenesis, as well as in Xiphophorus hybrid melanoma, similar to human tumours. Protein sequence comparison between fish and mammalian Mc1r revealed a remarkable concordance between evolutionary and functional analyses for the identification of residues and regions critical for receptor function.}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Selz, Y. and Braasch, I. and Hoffmann, C. and Schmidt, C. and Schultheis, C. and Schartl, M. and Volff, J. N.}, biburl = {https://www.bibsonomy.org/bibtex/2ef7170bbf8682bf0a4211c85edbea9c3/pharmawuerz}, endnotereftype = {Journal Article}, interhash = {086a392ea618f91e46c3bbe9fcd8d341}, intrahash = {ef7170bbf8682bf0a4211c85edbea9c3}, issn = {0378-1119 (Print) 0378-1119 (Linking)}, journal = {Gene}, keywords = {*Evolution, 1/chemistry/genetics/*metabolism Acid Amino Analysis, Animals Cell Cloning, Cyprinodontiformes/*genetics Data Dosage Experimental Gene Genetic Homology, Line Melanocortin, Melanocortins/agonists/genetics/metabolism Melanoma, Melanophores/metabolism Models, Molecular Oryzias/*genetics Phylogeny Protein Sequence Structure, Substitution Tertiary Type Zebrafish/*genetics Receptor}, month = {Oct 15}, note = {Selz, Yvonne Braasch, Ingo Hoffmann, Carsten Schmidt, Cornelia Schultheis, Christina Schartl, Manfred Volff, Jean-Nicolas Research Support, Non-U.S. Gov't Netherlands Gene Gene. 2007 Oct 15;401(1-2):114-22. Epub 2007 Jul 17.}, number = {1-2}, pages = {114-22}, shorttitle = {Evolution of melanocortin receptors in teleost fish: the melanocortin type 1 receptor}, timestamp = {2010-12-14T18:20:22.000+0100}, title = {Evolution of melanocortin receptors in teleost fish: the melanocortin type 1 receptor}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17707598}, volume = 401, year = 2007 }