%0 Journal Article %1 30 %A Cheng, Yuan-Yuan %A Li, Bing-Bing %A Li, Dao-Bo %A Chen, Jie-Jie %A Li, Wen-Wei %A Tong, Zhong-Hua %A Wu, Chao %A Yu, Han-Qing %D 2013 %I Public Library of Science %J PLoS ONE %K 30 %N 11 %P e78466 %R 10.1371/journal.pone.0078466 %T Promotion of Iron Oxide Reduction and Extracellular Electron Transfer in <italic>Shewanella oneidensis</italic> by DMSO %U http://dx.doi.org/10.1371%2Fjournal.pone.0078466 %V 8 %X <p>The dissimilatory metal reducing bacterium <italic>Shewanella oneidensis</italic> MR-1, known for its capacity of reducing iron and manganese oxides, has great environmental impacts. The iron oxides reducing process is affected by the coexistence of alternative electron acceptors in the environment, while investigation into it is limited so far. In this work, the impact of dimethyl sulphoxide (DMSO), a ubiquitous chemical in marine environment, on the reduction of hydrous ferric oxide (HFO) by <italic>S. oneidensis</italic> MR-1 was investigated. Results show that DMSO promoted HFO reduction by both wild type and Δ<italic>dmsE</italic>, but had no effect on the HFO reduction by Δ<italic>dmsB</italic>, indicating that such a promotion was dependent on the DMSO respiration. With the DMSO dosing, the levels of extracellular flavins and <italic>omcA</italic> expression were significantly increased in WT and further increased in Δ<italic>dmsE.</italic> Bioelectrochemical analysis show that DMSO also promoted the extracellular electron transfer of WT and Δ<italic>dmsE</italic>. These results demonstrate that DMSO could stimulate the HFO reduction through metabolic and genetic regulation in <italic>S. oneidensis</italic> MR-1, rather than compete for electrons with HFO. This may provide a potential respiratory pathway to enhance the microbial electron flows for environmental and engineering applications.</p>

@article{30, abstract = {<p>The dissimilatory metal reducing bacterium <italic>Shewanella oneidensis</italic> MR-1, known for its capacity of reducing iron and manganese oxides, has great environmental impacts. The iron oxides reducing process is affected by the coexistence of alternative electron acceptors in the environment, while investigation into it is limited so far. In this work, the impact of dimethyl sulphoxide (DMSO), a ubiquitous chemical in marine environment, on the reduction of hydrous ferric oxide (HFO) by <italic>S. oneidensis</italic> MR-1 was investigated. Results show that DMSO promoted HFO reduction by both wild type and Δ<italic>dmsE</italic>, but had no effect on the HFO reduction by Δ<italic>dmsB</italic>, indicating that such a promotion was dependent on the DMSO respiration. With the DMSO dosing, the levels of extracellular flavins and <italic>omcA</italic> expression were significantly increased in WT and further increased in Δ<italic>dmsE.</italic> Bioelectrochemical analysis show that DMSO also promoted the extracellular electron transfer of WT and Δ<italic>dmsE</italic>. These results demonstrate that DMSO could stimulate the HFO reduction through metabolic and genetic regulation in <italic>S. oneidensis</italic> MR-1, rather than compete for electrons with HFO. This may provide a potential respiratory pathway to enhance the microbial electron flows for environmental and engineering applications.</p>}, added-at = {2014-02-01T22:20:28.000+0100}, author = {Cheng, Yuan-Yuan and Li, Bing-Bing and Li, Dao-Bo and Chen, Jie-Jie and Li, Wen-Wei and Tong, Zhong-Hua and Wu, Chao and Yu, Han-Qing}, biburl = {https://www.bibsonomy.org/bibtex/21e32f635697c064662c53f8cee66105e/lepraunch}, description = {PLOS ONE: Promotion of Iron Oxide Reduction and Extracellular Electron Transfer in Shewanella oneidensis by DMSO}, doi = {10.1371/journal.pone.0078466}, interhash = {096e53425a022aab1038cef92bb03dba}, intrahash = {1e32f635697c064662c53f8cee66105e}, journal = {PLoS ONE}, keywords = {30}, month = {11}, number = 11, pages = {e78466}, publisher = {Public Library of Science}, timestamp = {2014-02-02T13:58:58.000+0100}, title = {Promotion of Iron Oxide Reduction and Extracellular Electron Transfer in <italic>Shewanella oneidensis</italic> by DMSO}, url = {http://dx.doi.org/10.1371%2Fjournal.pone.0078466}, volume = 8, year = 2013 }