%0 Journal Article %1 Dixelius.2003 %A Dixelius, J. %A Makinen, T. %A Wirzenius, M. %A Karkkainen, M. J. %A Wernstedt, C. %A Alitalo, K. %A Claesson-Welsh, L. %D 2003 %J J.Biol.Chem. %K Animals Binding Blood Cell Cells Chemistry Dimerization Electrophoresis Endothelial Endothelium Factor Factors Gel Growth Humans Immunoblotting Ligands Line Lymphatic Mutation Neovascularization Pathologic Peptides Phosphorylation Polyacrylamide Protein Receptor-2 Receptor-3 Research Signal Sites Structure Swine Tertiary Transduction Transfection Tyrosine Up-Regulation Vascular Vessels blood cells genetics metabolism pathology %N 42 %P 40973-40979 %T Ligand-induced vascular endothelial growth factor receptor-3 (VEGFR-3) heterodimerization with VEGFR-2 in primary lymphatic endothelial cells regulates tyrosine phosphorylation sites %U PM:12881528 %V 278 %X Vascular endothelial growth factors (VEGFs) regulate the development and growth of the blood and lymphatic vascular systems. Of the three VEGF receptors (VEGFR), VEGFR-1 and -2 are expressed on blood vessels; VEGFR-2 is found also on lymphatic vessels. VEGFR-3 is expressed mainly on lymphatic vessels but it is also up-regulated in tumor angiogenesis. Although VEGFR-3 is essential for proper lymphatic development, its signal transduction mechanisms are still incompletely understood. Trans-phosphorylation of activated, dimerized receptor tyrosine kinases is known to be critical for the regulation of kinase activity and for receptor interaction with signal transduction molecules. In this study, we have identified five tyrosyl phosphorylation sites in the VEGFR-3 carboxyl-terminal tail. These sites were used both in VEGFR-3 overexpressed in 293 cells and when the endogenous VEGFR-3 was activated in lymphatic endothelial cells. Interestingly, VEGF-C stimulation of lymphatic endothelial cell

@article{Dixelius.2003, abstract = {Vascular endothelial growth factors (VEGFs) regulate the development and growth of the blood and lymphatic vascular systems. Of the three VEGF receptors (VEGFR), VEGFR-1 and -2 are expressed on blood vessels; VEGFR-2 is found also on lymphatic vessels. VEGFR-3 is expressed mainly on lymphatic vessels but it is also up-regulated in tumor angiogenesis. Although VEGFR-3 is essential for proper lymphatic development, its signal transduction mechanisms are still incompletely understood. Trans-phosphorylation of activated, dimerized receptor tyrosine kinases is known to be critical for the regulation of kinase activity and for receptor interaction with signal transduction molecules. In this study, we have identified five tyrosyl phosphorylation sites in the VEGFR-3 carboxyl-terminal tail. These sites were used both in VEGFR-3 overexpressed in 293 cells and when the endogenous VEGFR-3 was activated in lymphatic endothelial cells. Interestingly, VEGF-C stimulation of lymphatic endothelial cell}, added-at = {2010-02-05T11:28:39.000+0100}, author = {Dixelius, J. and Makinen, T. and Wirzenius, M. and Karkkainen, M. J. and Wernstedt, C. and Alitalo, K. and Claesson-Welsh, L.}, biburl = {https://www.bibsonomy.org/bibtex/27fdf1d5d90849712adb9a624bdb96d4f/kanefendt}, interhash = {1abbdd68116dcc48c5f326b7761db3a8}, intrahash = {7fdf1d5d90849712adb9a624bdb96d4f}, journal = {J.Biol.Chem.}, keywords = {Animals Binding Blood Cell Cells Chemistry Dimerization Electrophoresis Endothelial Endothelium Factor Factors Gel Growth Humans Immunoblotting Ligands Line Lymphatic Mutation Neovascularization Pathologic Peptides Phosphorylation Polyacrylamide Protein Receptor-2 Receptor-3 Research Signal Sites Structure Swine Tertiary Transduction Transfection Tyrosine Up-Regulation Vascular Vessels blood cells genetics metabolism pathology}, number = 42, pages = {40973-40979}, timestamp = {2010-02-05T11:28:40.000+0100}, title = {Ligand-induced vascular endothelial growth factor receptor-3 (VEGFR-3) heterodimerization with VEGFR-2 in primary lymphatic endothelial cells regulates tyrosine phosphorylation sites}, url = {PM:12881528}, volume = 278, year = 2003 }