%0 Journal Article %1 Forsythe.1996 %A Forsythe, J. A. %A Jiang, B. H. %A Iyer, N. V. %A Agani, F. %A Leung, S. W. %A Koos, R. D. %A Semenza, G. L. %D 1996 %J Mol.Cell Biol. %K (Genetics) 1 40 A Acid Alignment Animals Base Binding Carcinoma Cell Cells Chemistry Cultured DNA-Binding Data Endothelial Expression Factor Factors Fusion Gene Genes Genetic Growth Hepatocellular Homology Human Humans Hypoxia Hypoxia-Inducible Liver Lymphokines Mice Molecular Neoplasms Nuclear Nucleic Promoter Proteins Rats Recombinant Regions Regulation Reporter Research Sequence Simian Subunit Transcription Transfection Tumor Vascular alpha biosynthesis cells genetics metabolism pathology physiology protein response virus %N 9 %P 4604-4613 %T Activation of vascular endothelial growth factor gene transcription by hypoxia-inducible factor 1 %U PM:8756616 %V 16 %X Expression of vascular endothelial growth factor (VEGF) is induced in cells exposed to hypoxia or ischemia. Neovascularization stimulated by VEGF occurs in several important clinical contexts, including myocardial ischemia, retinal disease, and tumor growth. Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric basic helix-loop-helix protein that activates transcription of the human erythropoietin gene in hypoxic cells. Here we demonstrate the involvement of HIF-1 in the activation of VEGF transcription. VEGF 5'-flanking sequences mediated transcriptional activation of reporter gene expression in hypoxic Hep3B cells. A 47-bp sequence located 985 to 939 bp 5' to the VEGF transcription initiation site mediated hypoxia-inducible reporter gene expression directed by a simian virus 40 promoter element that was otherwise minimally responsive to hypoxia. When reporters containing VEGF sequences, in the context of the native VEGF or heterologous simian virus 40 promoter, were cotransfected wit

@article{Forsythe.1996, abstract = {Expression of vascular endothelial growth factor (VEGF) is induced in cells exposed to hypoxia or ischemia. Neovascularization stimulated by VEGF occurs in several important clinical contexts, including myocardial ischemia, retinal disease, and tumor growth. Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric basic helix-loop-helix protein that activates transcription of the human erythropoietin gene in hypoxic cells. Here we demonstrate the involvement of HIF-1 in the activation of VEGF transcription. VEGF 5'-flanking sequences mediated transcriptional activation of reporter gene expression in hypoxic Hep3B cells. A 47-bp sequence located 985 to 939 bp 5' to the VEGF transcription initiation site mediated hypoxia-inducible reporter gene expression directed by a simian virus 40 promoter element that was otherwise minimally responsive to hypoxia. When reporters containing VEGF sequences, in the context of the native VEGF or heterologous simian virus 40 promoter, were cotransfected wit}, added-at = {2010-02-05T11:28:39.000+0100}, author = {Forsythe, J. A. and Jiang, B. H. and Iyer, N. V. and Agani, F. and Leung, S. W. and Koos, R. D. and Semenza, G. L.}, biburl = {https://www.bibsonomy.org/bibtex/24b070177a33afb0ef999fb3696696cad/kanefendt}, interhash = {1eebeaad88c29a00432696b5ddcf11bc}, intrahash = {4b070177a33afb0ef999fb3696696cad}, journal = {Mol.Cell Biol.}, keywords = {(Genetics) 1 40 A Acid Alignment Animals Base Binding Carcinoma Cell Cells Chemistry Cultured DNA-Binding Data Endothelial Expression Factor Factors Fusion Gene Genes Genetic Growth Hepatocellular Homology Human Humans Hypoxia Hypoxia-Inducible Liver Lymphokines Mice Molecular Neoplasms Nuclear Nucleic Promoter Proteins Rats Recombinant Regions Regulation Reporter Research Sequence Simian Subunit Transcription Transfection Tumor Vascular alpha biosynthesis cells genetics metabolism pathology physiology protein response virus}, number = 9, pages = {4604-4613}, timestamp = {2010-02-05T11:28:44.000+0100}, title = {Activation of vascular endothelial growth factor gene transcription by hypoxia-inducible factor 1}, url = {PM:8756616}, volume = 16, year = 1996 }