%0 Journal Article %1 claus_genetic_2005 %A Claus, Heike %A Maiden, Martin C J %A Wilson, Daniel J %A McCarthy, Noel D %A Jolley, Keith A %A Urwin, Rachel %A Hessler, Frank %A Frosch, Matthias %A Vogel, Ulrich %D 2005 %J The Journal of Infectious Diseases %K Adolescent, Adult, Age Alleles, Bacterial Capsules, Carrier Child, Distribution, Germany, Humans, Infections, Meningococcal Neisseria Preschool, Serotyping State, meningitidis, %N 8 %P 1263--1271 %R 10.1086/428590 %T Genetic analysis of meningococci carried by children and young adults %U http://www.ncbi.nlm.nih.gov/pubmed/15776372 %V 191 %X BACKGROUND Neisseria meningitidis is a diverse commensal bacterium that occasionally causes severe invasive disease. The relationship between meningococcal genotype and capsular polysaccharide, the principal virulence factor and vaccine component, was investigated in carried meningococci isolated from 8000 children and young adults in Bavaria, Germany. METHODS Of the 830 meningococci isolated (carriage rate, 10.4\%) by microbiological techniques, 822 were characterized by serogrouping, multilocus sequence typing, and genetic analysis of the capsule region. Statistical and population genetic analyses were applied to these data. RESULTS The rapid increase in carriage rates with age of carrier, the low prevalence of hyperinvasive meningococci, and the relative prevalence of the 4 disease-associated serogroups were consistent with earlier observations. There was no genetic structuring of the meningococcal population by age of carrier or sampling location; however, there was significant geographic structuring of the meningococci isolated in civil, but not military, institutions. The rate of capsule gene expression did not vary with age of carrier or meningococcal genotype, except for serogroup C, for which increased expression was associated with ST-11 (formerly ET-37) complex meningococci. CONCLUSIONS Serogroup C capsule expression during carriage may contribute to the invasive character of ST-11 complex meningococci and to the high efficacy of meningococcal serogroup C conjugate polysaccharide vaccine.

@article{claus_genetic_2005, abstract = {{BACKGROUND} Neisseria meningitidis is a diverse commensal bacterium that occasionally causes severe invasive disease. The relationship between meningococcal genotype and capsular polysaccharide, the principal virulence factor and vaccine component, was investigated in carried meningococci isolated from 8000 children and young adults in Bavaria, Germany. {METHODS} Of the 830 meningococci isolated (carriage rate, 10.4\%) by microbiological techniques, 822 were characterized by serogrouping, multilocus sequence typing, and genetic analysis of the capsule region. Statistical and population genetic analyses were applied to these data. {RESULTS} The rapid increase in carriage rates with age of carrier, the low prevalence of hyperinvasive meningococci, and the relative prevalence of the 4 disease-associated serogroups were consistent with earlier observations. There was no genetic structuring of the meningococcal population by age of carrier or sampling location; however, there was significant geographic structuring of the meningococci isolated in civil, but not military, institutions. The rate of capsule gene expression did not vary with age of carrier or meningococcal genotype, except for serogroup C, for which increased expression was associated with {ST-11} (formerly {ET-37)} complex meningococci. {CONCLUSIONS} Serogroup C capsule expression during carriage may contribute to the invasive character of {ST-11} complex meningococci and to the high efficacy of meningococcal serogroup C conjugate polysaccharide vaccine.}, added-at = {2011-06-24T11:21:47.000+0200}, author = {Claus, Heike and Maiden, Martin C J and Wilson, Daniel J and {McCarthy}, Noel D and Jolley, Keith A and Urwin, Rachel and Hessler, Frank and Frosch, Matthias and Vogel, Ulrich}, biburl = {https://www.bibsonomy.org/bibtex/2b62e1041f2e6bcd3417608ce9f5589ab/ag_vogel}, doi = {10.1086/428590}, interhash = {84c927dbe8b016190b642e0332e0c7ff}, intrahash = {b62e1041f2e6bcd3417608ce9f5589ab}, issn = {0022-1899}, journal = {The Journal of Infectious Diseases}, keywords = {Adolescent, Adult, Age Alleles, Bacterial Capsules, Carrier Child, Distribution, Germany, Humans, Infections, Meningococcal Neisseria Preschool, Serotyping State, meningitidis,}, month = apr, note = {{PMID:} 15776372}, number = 8, pages = {1263--1271}, timestamp = {2011-06-24T11:21:53.000+0200}, title = {Genetic analysis of meningococci carried by children and young adults}, url = {http://www.ncbi.nlm.nih.gov/pubmed/15776372}, volume = 191, year = 2005 }