Article,

COMPARISION OF TWO DNA EXTRACTION METHODS USING THE RT-PCR

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Reviews of Progress, (October 2013)

Abstract

The rapid development of molecular genetic analysis tools has made it possible to analyze most biological material even they are in a small quantity. Many different extraction protocols are being used to isolate DNA. However not all extraction methods perform equally well with heavily inhibited factors and sometimes is very important to choose an extraction procedure that could extract DNA efficiency and had the ability to minimize the amount of inhibitors co-extracted with the sample. Two different extraction methods were chosen: Chelex and Organic Extraction. To evaluate the efficiency of the procedures, quantity of DNA is compared using TaqMan Probe & Absolute Quantification method in ABI Prism RT-PCR. The samples used were blood samples. The results showed that with both methods extraction was efficiency for further analysis, but concentration of DNA was greater extracted with Chelex than it with Organic Extraction. Compare with Organic Extraction, Chelex is more efficient, more rapid, inexpensive, involves fewer steps and thus fewer opportunities for sample to sample contamination, no hazard chemicals are used.

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