%0 Journal Article %1 McCutchen1991 %A McCutchen, B. F. %A Choudary, P. V. %A Crenshaw, R. %A Maddox, D. %A Kamita, S. G. %A Palekar, N. %A Volrath, S. %A Fowler, E. %A Hammock, B. D. %A Maeda, S. %D 1991 %J Biotechnology (N Y) %K Animals; Baculoviridae, genetics; Base Sequence; Cell Line; Immunoblotting; Larva, drug effects; Lepidoptera, Molecular Sequence Data; Neurotoxins; Oligonucleotide Probes; Pest Control, methods; Recombinant Proteins, isolation /&/ purification/pharmacology; Recombination, Genetic; Restriction Mapping; Scorpion Venoms, genetics/isolation Transfection %N 9 %P 848--852 %T Development of a recombinant baculovirus expressing an insect-selective neurotoxin: potential for pest control. %V 9 %X Recombinant nuclear polyhedrosis viruses (NPVs) expressing insect-selective toxins, hormones, or enzymes could enhance their insecticidal properties. We have constructed a recombinant, polyhedrin-positive Autographa californica NPV (AcNPV) that is orally infectious and expresses an insect-selective toxin (AaIT), isolated from the scorpion Androctonus australis, under the control of the p10 promoter. Bioassays with the recombinant baculovirus on 2nd instar larvae of Heliothis virescens demonstrated a significant decrease in the time to kill (LT50 88.0 hours) compared to wild-type AcNPV (LT50 125 hours). Production of AaIT was confirmed by western blot analysis of larval hemolymph from infected H. virescens, and bioassays with larvae of Sarcophaga falculata.

@article{McCutchen1991, abstract = {Recombinant nuclear polyhedrosis viruses (NPVs) expressing insect-selective toxins, hormones, or enzymes could enhance their insecticidal properties. We have constructed a recombinant, polyhedrin-positive Autographa californica NPV (AcNPV) that is orally infectious and expresses an insect-selective toxin (AaIT), isolated from the scorpion Androctonus australis, under the control of the p10 promoter. Bioassays with the recombinant baculovirus on 2nd instar larvae of Heliothis virescens demonstrated a significant decrease in the time to kill (LT50 88.0 hours) compared to wild-type AcNPV (LT50 125 hours). Production of AaIT was confirmed by western blot analysis of larval hemolymph from infected H. virescens, and bioassays with larvae of Sarcophaga falculata.}, added-at = {2014-07-19T20:46:36.000+0200}, author = {McCutchen, B. F. and Choudary, P. V. and Crenshaw, R. and Maddox, D. and Kamita, S. G. and Palekar, N. and Volrath, S. and Fowler, E. and Hammock, B. D. and Maeda, S.}, biburl = {https://www.bibsonomy.org/bibtex/2b0c249d4b0498e659e387c95e7bb6ba4/ar0berts}, groups = {public}, institution = {Department of Entomology, University of California, Davis 95616.}, interhash = {9991754fa969730d166fb31d0838e183}, intrahash = {b0c249d4b0498e659e387c95e7bb6ba4}, journal = {Biotechnology (N Y)}, keywords = {Animals; Baculoviridae, genetics; Base Sequence; Cell Line; Immunoblotting; Larva, drug effects; Lepidoptera, Molecular Sequence Data; Neurotoxins; Oligonucleotide Probes; Pest Control, methods; Recombinant Proteins, isolation /&/ purification/pharmacology; Recombination, Genetic; Restriction Mapping; Scorpion Venoms, genetics/isolation Transfection}, language = {eng}, medline-pst = {ppublish}, month = Sep, number = 9, pages = {848--852}, pmid = {1367359}, timestamp = {2014-07-19T20:46:36.000+0200}, title = {Development of a recombinant baculovirus expressing an insect-selective neurotoxin: potential for pest control.}, username = {ar0berts}, volume = 9, year = 1991 }