%0 Journal Article %1 gerdes1983production %A Gerdes, Johannes %A Schwab, Ulrich %A Lemke, Hilmar %A Stein, Harald %D 1983 %I Wiley Subscription Services, Inc., A Wiley Company %J International Journal of Cancer %K ki67 methods phd %N 1 %P 13--20 %R 10.1002/ijc.2910310104 %T Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation %U http://dx.doi.org/10.1002/ijc.2910310104 %V 31 %X The production of a mouse monoclonal antibody, Ki-67, is described. The Ki-67 antibody recognized a nuclear antigen present in proliferating cells, but absent in resting cells. Immunostainings with Ki-67 revealed nuclear reactivity in cells of germinal centres of cortical follicles, cortical thymocytes, neck cells of gastrointestinal mucosa, un-differentiated spermatogonia and cells of a number of human cell lines. The Ki-67 antibody did not react with cells known to be in a resting stage, such as lymphocytes, monocytes, parietal cells and Paneth's cells of gastrointestinal mucosa, hepatocytes, renal cells, mature sperm cells, brain cells, etc. Expression of the antigen recognized by Ki-67 could be induced in peripheral blood lymphocytes after stimulation with phytohaemagglutinin, whereas it disappeared from HL-60 cells stimulated with phorbol esters to differentiate into mature macrophages in a resting stage. These findings suggest that Ki-67 is directed against a nuclear antigen associated with cell proliferation. A first series of immunostainings of tumour biopsies indicated that Ki-67 may be a potent tool for easy and quick evaluation of the proportion of proliferating cells in a tumour.

@article{gerdes1983production, abstract = {The production of a mouse monoclonal antibody, Ki-67, is described. The Ki-67 antibody recognized a nuclear antigen present in proliferating cells, but absent in resting cells. Immunostainings with Ki-67 revealed nuclear reactivity in cells of germinal centres of cortical follicles, cortical thymocytes, neck cells of gastrointestinal mucosa, un-differentiated spermatogonia and cells of a number of human cell lines. The Ki-67 antibody did not react with cells known to be in a resting stage, such as lymphocytes, monocytes, parietal cells and Paneth's cells of gastrointestinal mucosa, hepatocytes, renal cells, mature sperm cells, brain cells, etc. Expression of the antigen recognized by Ki-67 could be induced in peripheral blood lymphocytes after stimulation with phytohaemagglutinin, whereas it disappeared from HL-60 cells stimulated with phorbol esters to differentiate into mature macrophages in a resting stage. These findings suggest that Ki-67 is directed against a nuclear antigen associated with cell proliferation. A first series of immunostainings of tumour biopsies indicated that Ki-67 may be a potent tool for easy and quick evaluation of the proportion of proliferating cells in a tumour.}, added-at = {2015-06-03T08:57:11.000+0200}, author = {Gerdes, Johannes and Schwab, Ulrich and Lemke, Hilmar and Stein, Harald}, biburl = {https://www.bibsonomy.org/bibtex/27bc60db99783dd606d70100db8a56d4a/bkoch}, description = {Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation - Gerdes - 2006 - International Journal of Cancer - Wiley Online Library}, doi = {10.1002/ijc.2910310104}, interhash = {a6c2e299ff7fe665b18f05389c8bd874}, intrahash = {7bc60db99783dd606d70100db8a56d4a}, issn = {1097-0215}, journal = {International Journal of Cancer}, keywords = {ki67 methods phd}, number = 1, pages = {13--20}, publisher = {Wiley Subscription Services, Inc., A Wiley Company}, timestamp = {2015-06-03T08:57:11.000+0200}, title = {Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation}, url = {http://dx.doi.org/10.1002/ijc.2910310104}, volume = 31, year = 1983 }