%0 Journal Article %1 Bando.2004 %A Bando, H. %A Brokelmann, M. %A Toi, M. %A Alitalo, K. %A Sleeman, J. P. %A Sipos, B. %A Grone, H. J. %A Weich, H. A. %D 2004 %J Int.J.Cancer %K Animals Antibodies Assay Carcinoma Cells Chemistry Cultured Distribution Endothelial Enzyme-Linked Factor Goats Growth Human Humans Immunosorbent Kidney Ligands Lung Mice Monoclonal Neoplasms Neovascularization Pathologic RANGE Receptor-3 Research Sensitivity Specificity Tissue Tumor Vascular analysis and cells diagnostic immunology methods pathology protein use %N 2 %P 184-191 %T Immunodetection and quantification of vascular endothelial growth factor receptor-3 in human malignant tumor tissues %U PM:15197769 %V 111 %X Vascular endothelial growth factor receptor-3 (VEGFR-3) and its ligands, vascular endothelial growth factor-C (VEGF-C) and -D (VEGF-D), are the major molecules involved in developmental and pathological lymphangiogenesis. Here we describe for the first time the development of a specific indirect enzyme-linked immunosorbent assay (ELISA) for the quantification of VEGFR-3 in different human cell and tissue lysates. A combination of the goat polyclonal anti-VEGFR-3 antibody and the mouse monoclonal anti-human VEGFR-3 antibody was used. The assay was highly sensitive and reproducible with a detection range of 0.2-25 ng/ml. The assay was specific for VEGFR-3, with no cross-reactivity to VEGFR-1 or VEGFR-2. Complex formation with VEGF-C and VEGF-D had no effect on the sensitivity of the assay. The VEGFR-3 concentration in the lysates of cultured human dermal microvascular endothelial cells was 14-fold higher than in the lysates from human umbilical vein endothelial cells. In human kidney, br

@article{Bando.2004, abstract = {Vascular endothelial growth factor receptor-3 (VEGFR-3) and its ligands, vascular endothelial growth factor-C (VEGF-C) and -D (VEGF-D), are the major molecules involved in developmental and pathological lymphangiogenesis. Here we describe for the first time the development of a specific indirect enzyme-linked immunosorbent assay (ELISA) for the quantification of VEGFR-3 in different human cell and tissue lysates. A combination of the goat polyclonal anti-VEGFR-3 antibody and the mouse monoclonal anti-human VEGFR-3 antibody was used. The assay was highly sensitive and reproducible with a detection range of 0.2-25 ng/ml. The assay was specific for VEGFR-3, with no cross-reactivity to VEGFR-1 or VEGFR-2. Complex formation with VEGF-C and VEGF-D had no effect on the sensitivity of the assay. The VEGFR-3 concentration in the lysates of cultured human dermal microvascular endothelial cells was 14-fold higher than in the lysates from human umbilical vein endothelial cells. In human kidney, br}, added-at = {2010-02-05T11:28:39.000+0100}, author = {Bando, H. and Brokelmann, M. and Toi, M. and Alitalo, K. and Sleeman, J. P. and Sipos, B. and Grone, H. J. and Weich, H. A.}, biburl = {https://www.bibsonomy.org/bibtex/2351bf2b8227b115843af8eccc2fa1316/kanefendt}, interhash = {ac90174bf3dbb2a0c8cd16c5ccb9694b}, intrahash = {351bf2b8227b115843af8eccc2fa1316}, journal = {Int.J.Cancer}, keywords = {Animals Antibodies Assay Carcinoma Cells Chemistry Cultured Distribution Endothelial Enzyme-Linked Factor Goats Growth Human Humans Immunosorbent Kidney Ligands Lung Mice Monoclonal Neoplasms Neovascularization Pathologic RANGE Receptor-3 Research Sensitivity Specificity Tissue Tumor Vascular analysis and cells diagnostic immunology methods pathology protein use}, number = 2, pages = {184-191}, timestamp = {2010-02-05T11:28:43.000+0100}, title = {Immunodetection and quantification of vascular endothelial growth factor receptor-3 in human malignant tumor tissues}, url = {PM:15197769}, volume = 111, year = 2004 }