%0 Journal Article %1 Wallace2012446 %A Wallace, Lauren J. %A Boilard, Stephanie M.A.L. %A Eagle, Shannon H.C. %A Spall, Jennifer L. %A Shokralla, Shadi %A Hajibabaei, Mehrdad %D 2012 %J Food Research International %K DNA-Barcoding commercial phd %N 1 %P 446 - 452 %R 10.1016/j.foodres.2012.07.048 %T \DNA\ barcodes for everyday life: Routine authentication of Natural Health Products %U http://www.sciencedirect.com/science/article/pii/S096399691200292X %V 49 %X The authenticity of Natural Health Products (NHPs) remains an important legal, economic, health and conservation issue. Here, we use \DNA\ sequence information through standardized \DNA\ barcoding for the authentication of 95 \NHPs\ from animal and plant species of varying form and origin, with more extensive sampling for shark and ginseng products. We were able to identify 75% of all \NHPs\ tested, with success rates of up to 83% and 88% in shark and ginseng products, respectively. Standard \DNA\ barcode region of cytochrome C oxidase 1 (COI) gene and its smaller mini-barcode sequences were used for barcoding of all animal products. Although the rbcLa/matK core plant barcodes have limitations in robustly distinguishing Korean and American ginseng species, these species can be differentiated by a supplemental barcode from the Internal Transcribed Spacer (ITS) of nuclear ribosomal DNA. Interestingly, half of the samples commercially labelled as Korean ginseng were identified as American ginseng. This reveals a need for the authentication of ginseng-containing products. Ultimately, this work showcases the utility of \DNA\ barcodes for the real-world authentication of Natural Health Products.

@article{Wallace2012446, abstract = {The authenticity of Natural Health Products (NHPs) remains an important legal, economic, health and conservation issue. Here, we use \{DNA\} sequence information through standardized \{DNA\} barcoding for the authentication of 95 \{NHPs\} from animal and plant species of varying form and origin, with more extensive sampling for shark and ginseng products. We were able to identify 75% of all \{NHPs\} tested, with success rates of up to 83% and 88% in shark and ginseng products, respectively. Standard \{DNA\} barcode region of cytochrome C oxidase 1 (COI) gene and its smaller mini-barcode sequences were used for barcoding of all animal products. Although the rbcLa/matK core plant barcodes have limitations in robustly distinguishing Korean and American ginseng species, these species can be differentiated by a supplemental barcode from the Internal Transcribed Spacer (ITS) of nuclear ribosomal DNA. Interestingly, half of the samples commercially labelled as Korean ginseng were identified as American ginseng. This reveals a need for the authentication of ginseng-containing products. Ultimately, this work showcases the utility of \{DNA\} barcodes for the real-world authentication of Natural Health Products. }, added-at = {2013-12-16T19:23:46.000+0100}, author = {Wallace, Lauren J. and Boilard, Stephanie M.A.L. and Eagle, Shannon H.C. and Spall, Jennifer L. and Shokralla, Shadi and Hajibabaei, Mehrdad}, biburl = {https://www.bibsonomy.org/bibtex/2cf64f92e1fa2db1d97c21dc791a44ae4/drgirasol}, description = {DNA barcodes for everyday life: Routine authentication of Natural Health Products}, doi = {10.1016/j.foodres.2012.07.048}, interhash = {c30bad6128dcd93ab484ca8e14d94b64}, intrahash = {cf64f92e1fa2db1d97c21dc791a44ae4}, issn = {0963-9969}, journal = {Food Research International }, keywords = {DNA-Barcoding commercial phd}, number = 1, pages = {446 - 452}, timestamp = {2013-12-16T19:23:46.000+0100}, title = {\{DNA\} barcodes for everyday life: Routine authentication of Natural Health Products }, url = {http://www.sciencedirect.com/science/article/pii/S096399691200292X}, volume = 49, year = 2012 }