| Authors: |
N. C. Stellwagen
and C. Gelfi
and P. G. Righetti
|
| Tags: |
ACID
ACRYLAMIDO
CAPILLARIES
CAPILLARY
COATED
CONDENSATION;
COUNTERION
DIFFUSION-COEFFICIENTS;
ELECTROPHORESIS;
FRAGMENTS;
GEL
HIGHER
HYDROLYTIC
HYDROPHILICITY;
IMPROVED
LIGHT-SCATTERING;
MONOMERS;
PORE-SIZE;
RESTRICTION
STABILITY;
ZONE
|
| Abstract: |
The free solution mobility of DNP, has been measured by capillary
electrophoresis in the two buffers most commonly used for DNA gel
electrophoresis,;Tris-borate-EDTA (TEE) and Tris-acetate-EDTA (TAE).
The capillaries were coated with polymers of either of two novel
acrylamide monomers, N-acryloylaminoethoxethanol or N-acryloylaminopropanol,
both of which are stable at basic pH and effectively eliminate the
electroendosmotic,nobility due to the capillary walls. The free solution
mobility of DNA in TAE buffer was found to be (3.75 +/- 0.04) x 10(-4)
cm(2) V-1 s(-1) at 25 degrees C. independent of DNA concentration,
sample size, electric field strength, and capillary coating, and
in good agreement with other values in the literature. The free solution
mobility was independent of DNA molecular weight from similar to
400 base pairs to 48.5 kilobase pairs, but decreased monotonically
with decreasing molecular weight for smaller fragments. Surprisingly,
the free solution mobility of DNA in TEE buffer was found to be (4.5
+/- 0.1) x 10(-4) cm(2) V-1 s(-1), about 20% larger than observed
in TAE buffer, presumably because of the formation of nonspecific
borate-deoxyribose complexes. (C) 1997 John Wiley & Sons, Inc. |
@article{stellwagen97a,
title = {The free solution mobility of DNA},
author = {N. C. Stellwagen and C. Gelfi and P. G. Righetti},
journal = {Biopolymers},
month = {November},
number = {6},
pages = {687--703},
volume = {42},
year = {1997},
abstract = {The free solution mobility of DNP, has been measured by capillary
electrophoresis in the two buffers most commonly used for DNA gel
electrophoresis,;Tris-borate-EDTA (TEE) and Tris-acetate-EDTA (TAE).
The capillaries were coated with polymers of either of two novel
acrylamide monomers, N-acryloylaminoethoxethanol or N-acryloylaminopropanol,
both of which are stable at basic pH and effectively eliminate the
electroendosmotic,nobility due to the capillary walls. The free solution
mobility of DNA in TAE buffer was found to be (3.75 +/- 0.04) x 10(-4)
cm(2) V-1 s(-1) at 25 degrees C. independent of DNA concentration,
sample size, electric field strength, and capillary coating, and
in good agreement with other values in the literature. The free solution
mobility was independent of DNA molecular weight from similar to
400 base pairs to 48.5 kilobase pairs, but decreased monotonically
with decreasing molecular weight for smaller fragments. Surprisingly,
the free solution mobility of DNA in TEE buffer was found to be (4.5
+/- 0.1) x 10(-4) cm(2) V-1 s(-1), about 20% larger than observed
in TAE buffer, presumably because of the formation of nonspecific
borate-deoxyribose complexes. (C) 1997 John Wiley & Sons, Inc.},
owner = {grass}, sn = {0006-3525}, ut = {ISI:A1997YD66600007}, de = {DNA; free solution electrophoretic mobility; molecular weightEOLEOLdependence
of mobility; buffer dependence of mobility}, timestamp = {2007.06.11},
keywords = {ACID ACRYLAMIDO CAPILLARIES CAPILLARY COATED CONDENSATION; COUNTERION DIFFUSION-COEFFICIENTS; ELECTROPHORESIS; FRAGMENTS; GEL HIGHER HYDROLYTIC HYDROPHILICITY; IMPROVED LIGHT-SCATTERING; MONOMERS; PORE-SIZE; RESTRICTION STABILITY; ZONE }
}
::