Autoradiographic visualization of A1-adenosine receptors in brain
and peripheral tissues of rat and guinea pig using 125I-HPIA
Neurosci Lett, 87 (3):
215-20 (May 1988)Weber, R G Jones, C R Palacios, J M Lohse, M J Research Support,
Non-U.S. Gov't Netherlands Neuroscience letters Neurosci Lett. 1988
May 3;87(3):215-20..Abstract
A1-adenosine receptors were identified in sections of rat brain and
guinea pig kidney with the radioiodinated agonist 125I-N6-p-hydroxyphenylisopropyladenosine
(125I-HPIA) using in vitro autoradiography. The affinities of adenosine
receptor ligands in competing with 125I-HPIA binding to tissue sections
were in good agreement with those found in membranes, and indicate
that the binding site represents an A1-adenosine receptor. The distribution
of 125I-HPIA binding sites in rat brain sections was similar to the
pattern of 3HN6-cyclohexyladenosine (3HCHA) binding sites determined
previously, with highest densities in the hippocampus and dentate
gyrus, the cerebellar cortex, some thalamic nuclei and certain layers
of the cerebral cortex. In the guinea pig kidney 125I-HPIA labelled
longitudinal structures in the medulla. This study demonstrates that
125I-HPIA allows the autoradiographic detection of A1 adenosine receptors
in the brain and peripheral organs and has the advantage of short
exposure times.