%0 Journal Article %1 Zanor2009 %A Zanor, M. I. %A Osorio, S. %A Nunes-Nesi, A. %A Carrari, F. %A Lohse, M. %A Usadel, B. %A Kuhn, C. %A Bleiss, W. %A Giavalisco, P. %A Willmitzer, L. %A Sulpice, R. %A Zhou, Y. H. %A Fernie, A. R. %D 2009 %J Plant Physiol %K & Carbohydrate Fertility Fruit/genetics/growth Genetically Growth Interference Lycopersicon Metabolism/genetics Modified/metabolism Plant Plants, Pollen Proteins/genetics/metabolism/*physiology RNA Regulators/metabolism Seeds/genetics/metabolism Sucrose/*metabolism Tube/genetics/growth development/*metabolism development/metabolism esculentum/genetics/growth %N 3 %P 1204-18 %T RNA interference of LIN5 in tomato confirms its role in controlling Brix content, uncovers the influence of sugars on the levels of fruit hormones, and demonstrates the importance of sucrose cleavage for normal fruit development and fertility %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19439574 %V 150 %X It has been previously demonstrated, utilizing intraspecific introgression lines, that Lycopersicum Invertase5 (LIN5), which encodes a cell wall invertase, controls total soluble solids content in tomato (Solanum lycopersicum). The physiological role of this protein, however, has not yet been directly studied, since evaluation of data obtained from the introgression lines is complicated by the fact that they additionally harbor many other wild species alleles. To allow a more precise comparison, we generated transgenic tomato in which we silenced the expression of LIN5 using the RNA interference approach. The transformants were characterized by an altered flower and fruit morphology, displaying increased numbers of petals and sepals per flower, an increased rate of fruit abortion, and a reduction in fruit size. Evaluation of the mature fruit revealed that the transformants were characterized by a reduction of seed number per plant. Furthermore, detailed physiological analysis revealed that the transformants displayed aberrant pollen morphology and a reduction in the rate of pollen tube elongation. Metabolite profiling of ovaries and green and red fruit revealed that metabolic changes in the transformants were largely confined to sugar metabolism, whereas transcript and hormone profiling revealed broad changes both in the hormones themselves and in transcripts encoding their biosynthetic enzymes and response elements. These results are discussed in the context of current understanding of the role of sugar during the development of tomato fruit, with particular focus given to its impact on hormone levels and organ morphology.

@article{Zanor2009, abstract = {It has been previously demonstrated, utilizing intraspecific introgression lines, that Lycopersicum Invertase5 (LIN5), which encodes a cell wall invertase, controls total soluble solids content in tomato (Solanum lycopersicum). The physiological role of this protein, however, has not yet been directly studied, since evaluation of data obtained from the introgression lines is complicated by the fact that they additionally harbor many other wild species alleles. To allow a more precise comparison, we generated transgenic tomato in which we silenced the expression of LIN5 using the RNA interference approach. The transformants were characterized by an altered flower and fruit morphology, displaying increased numbers of petals and sepals per flower, an increased rate of fruit abortion, and a reduction in fruit size. Evaluation of the mature fruit revealed that the transformants were characterized by a reduction of seed number per plant. Furthermore, detailed physiological analysis revealed that the transformants displayed aberrant pollen morphology and a reduction in the rate of pollen tube elongation. Metabolite profiling of ovaries and green and red fruit revealed that metabolic changes in the transformants were largely confined to sugar metabolism, whereas transcript and hormone profiling revealed broad changes both in the hormones themselves and in transcripts encoding their biosynthetic enzymes and response elements. These results are discussed in the context of current understanding of the role of sugar during the development of tomato fruit, with particular focus given to its impact on hormone levels and organ morphology.}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Zanor, M. I. and Osorio, S. and Nunes-Nesi, A. and Carrari, F. and Lohse, M. and Usadel, B. and Kuhn, C. and Bleiss, W. and Giavalisco, P. and Willmitzer, L. and Sulpice, R. and Zhou, Y. H. and Fernie, A. R.}, biburl = {https://www.bibsonomy.org/bibtex/21817fbdbc71035116855ff2d5426c25d/pharmawuerz}, endnotereftype = {Journal Article}, interhash = {7c4e4f821abed1596b54e60edbdfdb05}, intrahash = {1817fbdbc71035116855ff2d5426c25d}, issn = {0032-0889 (Print) 0032-0889 (Linking)}, journal = {Plant Physiol}, keywords = {& Carbohydrate Fertility Fruit/genetics/growth Genetically Growth Interference Lycopersicon Metabolism/genetics Modified/metabolism Plant Plants, Pollen Proteins/genetics/metabolism/*physiology RNA Regulators/metabolism Seeds/genetics/metabolism Sucrose/*metabolism Tube/genetics/growth development/*metabolism development/metabolism esculentum/genetics/growth}, month = Jul, note = {Zanor, Maria Ines Osorio, Sonia Nunes-Nesi, Adriano Carrari, Fernando Lohse, Marc Usadel, Bjorn Kuhn, Christina Bleiss, Wilfrid Giavalisco, Patrick Willmitzer, Lothar Sulpice, Ronan Zhou, Yan-Hong Fernie, Alisdair R Research Support, Non-U.S. Gov't United States Plant physiology Plant Physiol. 2009 Jul;150(3):1204-18. Epub 2009 May 13.}, number = 3, pages = {1204-18}, shorttitle = {RNA interference of LIN5 in tomato confirms its role in controlling Brix content, uncovers the influence of sugars on the levels of fruit hormones, and demonstrates the importance of sucrose cleavage for normal fruit development and fertility}, timestamp = {2010-12-14T18:12:37.000+0100}, title = {RNA interference of LIN5 in tomato confirms its role in controlling Brix content, uncovers the influence of sugars on the levels of fruit hormones, and demonstrates the importance of sucrose cleavage for normal fruit development and fertility}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19439574}, volume = 150, year = 2009 }