BibSonomy :: publication ::

tag user group author concept BibTeX key search:all search:microbio

The blue social bookmark and publication sharing system.

entry of microbio:

(0)

This publication has not been reviewed yet.

rating distribution

average user rating

The average rating is computed over all reviews. However, some of them may be invisible to you due to the visibility setting chosen by the reviewers.

(0.0 of 5.0 based on 0 reviews)

Functioning and robustness of a bacterial circadian clock

by: Sebastien Clodong, Ulf Duehring, Luiza Kronk, Annegret Wilde, Ilka Axmann, Hanspeter Herzel, and Markus Kollmann

In: MOLECULAR SYSTEMS BIOLOGY, Vol. 3 (2007) .

Abstract

Cyanobacteria are the simplest known cellular systems that regulate their biological activities in daily cycles. For the cyanobacterium Synechococcus elongatus, it has been shown by in vitro and in vivo experiments that the basic circadian timing process is based on rhythmic phosphorylation of KaiC hexamers. Despite the excellent experimental work, a full systems level understanding of the in vitro clock is still lacking. In this work, we provide a mathematical approach to scan different hypothetical mechanisms for the primary circadian oscillator, starting from experimentally established molecular properties of the clock proteins. Although optimised for highest performance, only one of the in silico-generated reaction networks was able to reproduce the experimentally found high amplitude and robustness against perturbations. In this reaction network, a negative feedback synchronises the phosphorylation level of the individual hexamers and has indeed been realised in S. elongatus by KaiA sequestration as confirmed by experiments.

BibTeX record

@article{ISI:000245381300002,
  abstract = {Cyanobacteria are the simplest known cellular systems that regulate
their biological activities in daily cycles. For the cyanobacterium
Synechococcus elongatus, it has been shown by in vitro and in vivo
experiments that the basic circadian timing process is based on
rhythmic phosphorylation of KaiC hexamers. Despite the excellent
experimental work, a full systems level understanding of the in vitro
clock is still lacking. In this work, we provide a mathematical
approach to scan different hypothetical mechanisms for the primary
circadian oscillator, starting from experimentally established
molecular properties of the clock proteins. Although optimised for
highest performance, only one of the in silico-generated reaction
networks was able to reproduce the experimentally found high amplitude
and robustness against perturbations. In this reaction network, a
negative feedback synchronises the phosphorylation level of the
individual hexamers and has indeed been realised in S. elongatus by
KaiA sequestration as confirmed by experiments.},
  added-at = {2008-07-15T11:10:10.000+0200},
  author = {Clodong, Sebastien and Duehring, Ulf and Kronk, Luiza and Wilde, Annegret and Axmann, Ilka and Herzel, Hanspeter and Kollmann, Markus},
  biburl = {http://www.bibsonomy.org/bibtex/24416df445cea52104905722029172e49/microbio},
  interhash = {6767cb27f732e7cf313935084f19cea2},
  intrahash = {4416df445cea52104905722029172e49},
  issn = {1744-4292},
  journal = {MOLECULAR SYSTEMS BIOLOGY},
  keywords = {IFZ circadian_clock cyanobacteria networks robustness systems_biology},
  timestamp = {2008-07-15T11:10:10.000+0200},
  title = {Functioning and robustness of a bacterial circadian clock},
  volume = 3,
  year = 2007
}

Endnote record

%0 Journal Article
%1 ISI:000245381300002
%A Clodong, Sebastien
%A Duehring, Ulf
%A Kronk, Luiza
%A Wilde, Annegret
%A Axmann, Ilka
%A Herzel, Hanspeter
%A Kollmann, Markus
%D 2007
%J MOLECULAR SYSTEMS BIOLOGY
%K 
%T Functioning and robustness of a bacterial circadian clock
%V 3
%X Cyanobacteria are the simplest known cellular systems that regulate
their biological activities in daily cycles. For the cyanobacterium
Synechococcus elongatus, it has been shown by in vitro and in vivo
experiments that the basic circadian timing process is based on
rhythmic phosphorylation of KaiC hexamers. Despite the excellent
experimental work, a full systems level understanding of the in vitro
clock is still lacking. In this work, we provide a mathematical
approach to scan different hypothetical mechanisms for the primary
circadian oscillator, starting from experimentally established
molecular properties of the clock proteins. Although optimised for
highest performance, only one of the in silico-generated reaction
networks was able to reproduce the experimentally found high amplitude
and robustness against perturbations. In this reaction network, a
negative feedback synchronises the phosphorylation level of the
individual hexamers and has indeed been realised in S. elongatus by
KaiA sequestration as confirmed by experiments.