%0 Journal Article %1 Chen:2015:Cold-Spring-Harb-Protoc:25762417 %A Chen, R %A Im, H %A Snyder, M %D 2015 %J Cold Spring Harb Protoc %K MUSTREAD agilent dna-sequencing exome fulltext methods sureselect wes %N 7 %P 626-633 %R 10.1101/pdb.prot083659 %T Whole-Exome Enrichment with the Agilent SureSelect Human All Exon Platform %U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490097/ %V 2015 %X There are multiple platforms available for whole-exome enrichment and sequencing (WES). This protocol is based on the Agilent SureSelect Human All Exon platform, which targets ∼50 Mb of the human exonic regions. The SureSelect system uses ∼120-base RNA probes to capture known coding DNA sequences (CDS) from the NCBI Consensus CDS Database as well as other major RNA coding sequence databases, such as Sanger miRBase. The protocol can be performed at the benchside without the need for automation, and the resulting library can be used for targeted next-generation sequencing on an Illumina HiSeq 2000 sequencer.

@article{Chen:2015:Cold-Spring-Harb-Protoc:25762417, abstract = {There are multiple platforms available for whole-exome enrichment and sequencing (WES). This protocol is based on the Agilent SureSelect Human All Exon platform, which targets ∼50 Mb of the human exonic regions. The SureSelect system uses ∼120-base RNA probes to capture known coding DNA sequences (CDS) from the NCBI Consensus CDS Database as well as other major RNA coding sequence databases, such as Sanger miRBase. The protocol can be performed at the benchside without the need for automation, and the resulting library can be used for targeted next-generation sequencing on an Illumina HiSeq 2000 sequencer.}, added-at = {2017-10-03T09:34:38.000+0200}, author = {Chen, R and Im, H and Snyder, M}, biburl = {https://www.bibsonomy.org/bibtex/276d33fdc3fbdcb31e6f077fa9d6b12c5/marcsaric}, description = {Whole-Exome Enrichment with the Agilent SureSelect Human All Exon Platform}, doi = {10.1101/pdb.prot083659}, interhash = {ace2fbce5dd34a85c2c88b7ab0302dc4}, intrahash = {76d33fdc3fbdcb31e6f077fa9d6b12c5}, journal = {Cold Spring Harb Protoc}, keywords = {MUSTREAD agilent dna-sequencing exome fulltext methods sureselect wes}, month = mar, number = 7, pages = {626-633}, pmid = {25762417}, timestamp = {2017-10-03T09:34:38.000+0200}, title = {Whole-Exome Enrichment with the Agilent SureSelect Human All Exon Platform}, url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490097/}, volume = 2015, year = 2015 }