%0 Journal Article %1 harmsen_dna_2005 %A Harmsen, Dag %A Claus, Heike %A Vogel, Ulrich %D 2005 %J International Journal of Medical Microbiology: IJMM %K A, Adhesins, Bacterial Bacterial, Humans, Infections, Methicillin Methicillin, Protein Repeat Resistance, Sequences Staphylococcal Staphylococcus Tandem Techniques, Typing aureus, {DNA}, %N 8 %P 525--528 %T DNA sequence-based tandem repeat analysis of the clfB gene is less discriminatory than spa typing for methicillin-resistant Staphylococcus aureus %U http://www.ncbi.nlm.nih.gov/pubmed/15790297 %V 294 %X Methicillin-resistant Staphylococcus aureus (MRSA) are a major challenge for hospital hygiene. Typing by DNA sequence analysis of the repeat region of the protein A gene (spa) significantly improved typing of MRSA in the hospital setting. However, microevolution of spa repeats in epidemic clones appears to occur at a fairly slow clock rate. Therefore, DNA sequence-based methods providing additional resolution are desirable in some situations. We evaluated the use of the clfB repeat region proposed recently by others as a possible complementation to spa typing. Using epidemic MRSA isolates from two German university hospitals, we show that the clfB repeat region does not offer any additional discriminatory power.

@article{harmsen_dna_2005, abstract = {Methicillin-resistant Staphylococcus aureus {(MRSA)} are a major challenge for hospital hygiene. Typing by {DNA} sequence analysis of the repeat region of the protein A gene (spa) significantly improved typing of {MRSA} in the hospital setting. However, microevolution of spa repeats in epidemic clones appears to occur at a fairly slow clock rate. Therefore, {DNA} sequence-based methods providing additional resolution are desirable in some situations. We evaluated the use of the {clfB} repeat region proposed recently by others as a possible complementation to spa typing. Using epidemic {MRSA} isolates from two German university hospitals, we show that the {clfB} repeat region does not offer any additional discriminatory power.}, added-at = {2011-06-24T11:21:47.000+0200}, author = {Harmsen, Dag and Claus, Heike and Vogel, Ulrich}, biburl = {https://www.bibsonomy.org/bibtex/252279d19c848ec4089f099dea63699cb/ag_vogel}, interhash = {2799ae0a7ee5c5502ca27ade48200e0b}, intrahash = {52279d19c848ec4089f099dea63699cb}, issn = {1438-4221}, journal = {International Journal of Medical Microbiology: {IJMM}}, keywords = {A, Adhesins, Bacterial Bacterial, Humans, Infections, Methicillin Methicillin, Protein Repeat Resistance, Sequences Staphylococcal Staphylococcus Tandem Techniques, Typing aureus, {DNA},}, month = mar, note = {{PMID:} 15790297}, number = 8, pages = {525--528}, timestamp = {2011-06-24T11:21:51.000+0200}, title = {{DNA} sequence-based tandem repeat analysis of the {clfB} gene is less discriminatory than spa typing for methicillin-resistant Staphylococcus aureus}, url = {http://www.ncbi.nlm.nih.gov/pubmed/15790297}, volume = 294, year = 2005 }