Article,
SELDI-TOF-MS analysis of chloroquine resistant and sensitive Plasmodium falciparum strains
PROTEOMICS, 7 (5): 711-721 (2007)
Abstract
The resistance of the malarial parasite Plasmodium falciparum to
chloroquine represents an emerging problem since neither mode of drug
action nor mechanisms of resistance are fully elucidated. We describe a
protein expression profiling approach by SELDI-TOF-MS as a useful tool
for studying the proteome of malarial parasites. Reproducible and
complex protein profiles of the P. falciparum strains K1, Dd2, HB3 and
3D7 were measured on four array types. Hierarchical clustering led to a
clear separation of the two major subgroups ��resistant" and
��sensitive" as well as of the four parasite strains. Our study
delivers sets of regulated proteins derived from extensive comparative
analyses of 64 P.falciparum protein profiles. A group of 12 peaks
reflecting proteome changes under chloroquine treatment and a set of 10
potential chloroquine resistance markers were defined. Three of these
regulated peaks were preparatively enriched, purified and identified.
They were shown to represent the plasmo
