%0 Journal Article %1 Salaverria.2013 %A Salaverria, Itziar %A Martin-Guerrero, Idoia %A Burkhardt, Birgit %A Kreuz, Markus %A Zenz, Thorsten %A Oschlies, Ilske %A Arnold, Norbert %A Baudis, Michael %A Bens, Susanne %A García-Orad, Africa %A Lisfeld, Jasmin %A Schwaenen, Carsten %A Szczepanowski, Monika %A Wessendorf, Swen %A Pfreundschuh, Michael %A Trümper, Lorenz %A Klapper, Wolfram %A Siebert, Reiner %D 2013 %J Genes, chromosomes & cancer %K Adolescent Adult Aged Aged,_80_and_over Base_Sequence Child Child,_Preschool Chromosome_Aberrations Comparative_Genomic_Hybridization/methods DNA_Copy_Number_Variations DNA_Mutational_Analysis Female Germinal_Center/metabolism/pathology Humans In_Situ_Hybridization,_Fluorescence Interferon_Regulatory_Factors/genetics Lymphoma,_Follicular/genetics/metabolism/pathology Lymphoma,_Large_B-Cell,_Diffuse/genetics/pathology Male Middle_Aged Point_Mutation Translocation,_Genetic Tumor_Suppressor_Protein_p53/genetics Young_Adult %N 2 %P 150–155 %T High resolution copy number analysis of IRF4 translocation-positive diffuse large B-cell and follicular lymphomas %V 52 %X Translocations affecting chromosome subband 6p25.3 containing the IRF4 gene have been recently described as characteristic alterations in a molecularly distinct subset of germinal center B-cell-derived lymphomas. Secondary changes have yet only been described in few of these lymphomas. Here, we performed array-comparative genomic hybridization and molecular inversion probe microarray analyses on DNA from 12 formalin-fixed paraffin-embedded and two fresh-frozen IRF4 translocation-positive lymphomas, which together with the previously published data on nine cases allowed the extension of copy number analyses to a total of 23 of these lymphomas. All except one case carried chromosomal imbalances, most frequently gains in Xq28, 11q22.3-qter, and 7q32.1-qter and losses in 6q13-16.1, 15q14-22.31, and 17p. No recurrent copy-neutral losses of heterozygosity were observed. TP53 point mutations were detected in three of six cases with loss of 17p. Overall this study unravels a recurrent pattern of secondary genetic alterations in IRF4 translocation-positive lymphomas.

@article{Salaverria.2013, abstract = {Translocations affecting chromosome subband 6p25.3 containing the IRF4 gene have been recently described as characteristic alterations in a molecularly distinct subset of germinal center B-cell-derived lymphomas. Secondary changes have yet only been described in few of these lymphomas. Here, we performed array-comparative genomic hybridization and molecular inversion probe microarray analyses on DNA from 12 formalin-fixed paraffin-embedded and two fresh-frozen IRF4 translocation-positive lymphomas, which together with the previously published data on nine cases allowed the extension of copy number analyses to a total of 23 of these lymphomas. All except one case carried chromosomal imbalances, most frequently gains in Xq28, 11q22.3-qter, and 7q32.1-qter and losses in 6q13-16.1, 15q14-22.31, and 17p. No recurrent copy-neutral losses of heterozygosity were observed. TP53 point mutations were detected in three of six cases with loss of 17p. Overall this study unravels a recurrent pattern of secondary genetic alterations in IRF4 translocation-positive lymphomas.}, added-at = {2014-10-13T18:24:59.000+0200}, author = {Salaverria, Itziar and Martin-Guerrero, Idoia and Burkhardt, Birgit and Kreuz, Markus and Zenz, Thorsten and Oschlies, Ilske and Arnold, Norbert and Baudis, Michael and Bens, Susanne and García-Orad, Africa and Lisfeld, Jasmin and Schwaenen, Carsten and Szczepanowski, Monika and Wessendorf, Swen and Pfreundschuh, Michael and Trümper, Lorenz and Klapper, Wolfram and Siebert, Reiner}, biburl = {https://www.bibsonomy.org/bibtex/286560098433d308893bdc96a8e6b3e76/drtester}, interhash = {0fe1c8a9288521726da7233732669787}, intrahash = {86560098433d308893bdc96a8e6b3e76}, journal = {Genes, chromosomes \& cancer}, keywords = {Adolescent Adult Aged Aged,_80_and_over Base_Sequence Child Child,_Preschool Chromosome_Aberrations Comparative_Genomic_Hybridization/methods DNA_Copy_Number_Variations DNA_Mutational_Analysis Female Germinal_Center/metabolism/pathology Humans In_Situ_Hybridization,_Fluorescence Interferon_Regulatory_Factors/genetics Lymphoma,_Follicular/genetics/metabolism/pathology Lymphoma,_Large_B-Cell,_Diffuse/genetics/pathology Male Middle_Aged Point_Mutation Translocation,_Genetic Tumor_Suppressor_Protein_p53/genetics Young_Adult}, number = 2, pages = {150–155}, timestamp = {2014-10-13T18:24:59.000+0200}, title = {High resolution copy number analysis of IRF4 translocation-positive diffuse large B-cell and follicular lymphomas}, volume = 52, year = 2013 }