%0 Journal Article %1 Scholl3788 %A Scholl, Christina %A Kübert, Natalie %A Muenz, Thomas S. %A Rössler, Wolfgang %D 2015 %I The Company of Biologists Ltd %J Journal of Experimental Biology %K ag_roessler zoo_2 %N 23 %P 3788--3796 %R 10.1242/jeb.124859 %T CaMKII knockdown affects both early and late phases of olfactory long-term memory in the honeybee %U http://jeb.biologists.org/content/218/23/3788 %V 218 %X Honeybees are able to solve complex learning tasks and memorize learned information for long time periods. The molecular mechanisms mediating long-term memory (LTM) in the honeybee Apis mellifera are, to a large part, still unknown. We approached this question by investigating the potential function of the calcium/calmodulin-dependent protein kinase II (CaMKII), an enzyme known as a ‘molecular memory switch’ in vertebrates. CaMKII is able to switch to a calcium-independent constitutively active state, providing a mechanism for a molecular memory and has further been shown to play an essential role in structural synaptic plasticity. Using a combination of knockdown by RNA interference and pharmacological manipulation, we disrupted the function of CaMKII during olfactory learning and memory formation. We found that learning, memory acquisition and mid-term memory were not affected, but all manipulations consistently resulted in an impaired LTM. Both early LTM (24 h after learning) and late LTM (72 h after learning) were significantly disrupted, indicating the necessity of CaMKII in two successive stages of LTM formation in the honeybee.

@article{Scholl3788, abstract = {Honeybees are able to solve complex learning tasks and memorize learned information for long time periods. The molecular mechanisms mediating long-term memory (LTM) in the honeybee Apis mellifera are, to a large part, still unknown. We approached this question by investigating the potential function of the calcium/calmodulin-dependent protein kinase II (CaMKII), an enzyme known as a {\textquoteleft}molecular memory switch{\textquoteright} in vertebrates. CaMKII is able to switch to a calcium-independent constitutively active state, providing a mechanism for a molecular memory and has further been shown to play an essential role in structural synaptic plasticity. Using a combination of knockdown by RNA interference and pharmacological manipulation, we disrupted the function of CaMKII during olfactory learning and memory formation. We found that learning, memory acquisition and mid-term memory were not affected, but all manipulations consistently resulted in an impaired LTM. Both early LTM (24 h after learning) and late LTM (72 h after learning) were significantly disrupted, indicating the necessity of CaMKII in two successive stages of LTM formation in the honeybee.}, added-at = {2017-04-04T14:29:24.000+0200}, author = {Scholl, Christina and Kübert, Natalie and Muenz, Thomas S. and Rössler, Wolfgang}, biburl = {https://www.bibsonomy.org/bibtex/2927d15c54e1a51ac794cdfe4e5be4350/zoologieii}, doi = {10.1242/jeb.124859}, eprint = {http://jeb.biologists.org/content/218/23/3788.full.pdf}, interhash = {fba9c58a9d05b25c57c2d6da4a2fa471}, intrahash = {927d15c54e1a51ac794cdfe4e5be4350}, issn = {0022-0949}, journal = {Journal of Experimental Biology}, keywords = {ag_roessler zoo_2}, number = 23, pages = {3788--3796}, publisher = {The Company of Biologists Ltd}, timestamp = {2023-07-04T14:45:49.000+0200}, title = {CaMKII knockdown affects both early and late phases of olfactory long-term memory in the honeybee}, url = {http://jeb.biologists.org/content/218/23/3788}, volume = 218, year = 2015 }