Monitoring of cAMP synthesis and degradation in living cells
V. Nikolaev, and M. Lohse. Physiology (Bethesda), (April 2006)Nikolaev, Viacheslav O Lohse, Martin J Review United States Physiology
(Bethesda, Md.) Physiology (Bethesda). 2006 Apr;21:86-92..
Abstract
cAMP is an important second messenger with a plethora of cellular
effects and biological roles. To monitor and visualize cAMP in intact
living cells, electrophysiological and fluorescent methods have been
developed based on activation of all three types of cAMP effectors:
protein kinase A, cyclic nucleotide-gated channels, and exchange
protein directly activated by cAMP. In this review, we describe and
compare these techniques in terms of their robustness, sensitivity
and spatio-temporal resolution.
%0 Journal Article
%1 Nikolaev2006c
%A Nikolaev, V. O.
%A Lohse, M. J.
%D 2006
%J Physiology (Bethesda)
%K AMP-Dependent AMP/analysis/*metabolism Animals Antiporters/physiology Biosensing Calcium/physiology Cation Cells/chemistry/*metabolism Channels Channels/physiology Cyclic Cytosol/chemistry Electrophysiology/*methods Exchange Factors/analysis/physiology Fluorescence/*methods Guanine Humans Hydrolysis Ion Kinases/physiology Microscopy, Nucleotide Nucleotide-Gated Protein Radioimmunoassay Techniques
%P 86-92
%T Monitoring of cAMP synthesis and degradation in living cells
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16565474
%V 21
%X cAMP is an important second messenger with a plethora of cellular
effects and biological roles. To monitor and visualize cAMP in intact
living cells, electrophysiological and fluorescent methods have been
developed based on activation of all three types of cAMP effectors:
protein kinase A, cyclic nucleotide-gated channels, and exchange
protein directly activated by cAMP. In this review, we describe and
compare these techniques in terms of their robustness, sensitivity
and spatio-temporal resolution.
@article{Nikolaev2006c,
abstract = {cAMP is an important second messenger with a plethora of cellular
effects and biological roles. To monitor and visualize cAMP in intact
living cells, electrophysiological and fluorescent methods have been
developed based on activation of all three types of cAMP effectors:
protein kinase A, cyclic nucleotide-gated channels, and exchange
protein directly activated by cAMP. In this review, we describe and
compare these techniques in terms of their robustness, sensitivity
and spatio-temporal resolution.},
added-at = {2010-12-14T18:12:02.000+0100},
author = {Nikolaev, V. O. and Lohse, M. J.},
biburl = {https://www.bibsonomy.org/bibtex/295826cf983fe7ba996de4f12458d4e02/pharmawuerz},
endnotereftype = {Journal Article},
interhash = {58424c782b936c3de31000716ddaba2d},
intrahash = {95826cf983fe7ba996de4f12458d4e02},
issn = {1548-9213 (Print) 1548-9213 (Linking)},
journal = {Physiology (Bethesda)},
keywords = {AMP-Dependent AMP/analysis/*metabolism Animals Antiporters/physiology Biosensing Calcium/physiology Cation Cells/chemistry/*metabolism Channels Channels/physiology Cyclic Cytosol/chemistry Electrophysiology/*methods Exchange Factors/analysis/physiology Fluorescence/*methods Guanine Humans Hydrolysis Ion Kinases/physiology Microscopy, Nucleotide Nucleotide-Gated Protein Radioimmunoassay Techniques},
month = Apr,
note = {Nikolaev, Viacheslav O Lohse, Martin J Review United States Physiology
(Bethesda, Md.) Physiology (Bethesda). 2006 Apr;21:86-92.},
pages = {86-92},
shorttitle = {Monitoring of cAMP synthesis and degradation in living cells},
timestamp = {2010-12-14T18:12:27.000+0100},
title = {Monitoring of cAMP synthesis and degradation in living cells},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16565474},
volume = 21,
year = 2006
}