Scientific cinematography using ultrafast optical imaging is a common tool to study motion. In opaque organisms or structures, X-ray radiography captures sequences of 2D projections to visualize morphological dynamics, but for many applications full four-dimensional (4D) spatiotemporal information is highly desirable. We introduce in vivo X-ray cine-tomography as a 4D imaging technique developed to study real-time dynamics in small living organisms with micrometer spatial resolution and subsecond time resolution. The method enables insights into the physiology of small animals by tracking the 4D morphological dynamics of minute anatomical features as demonstrated in this work by the analysis of fast-moving screw-and-nut–type weevil hip joints. The presented method can be applied to a broad range of biological specimens and biotechnological processes.
%0 Journal Article
%1 dosSantosRolo2014
%A dos Santos Rolo, Tomy
%A Ershov, Alexey
%A van de Kamp, Thomas
%A Baumbach, Tilo
%D 2014
%J Proceedings of the National Academy of Sciences
%K cine-tomography in-vivo_imaging morphological_dynanics motion_tracking myown screw_joint x-ray
%N 11
%P 3921-3926
%R 10.1073/pnas.1308650111
%T In vivo X-ray cine-tomography for tracking morphological dynamics
%U http://www.pnas.org/content/111/11/3921.abstract
%V 111
%X Scientific cinematography using ultrafast optical imaging is a common tool to study motion. In opaque organisms or structures, X-ray radiography captures sequences of 2D projections to visualize morphological dynamics, but for many applications full four-dimensional (4D) spatiotemporal information is highly desirable. We introduce in vivo X-ray cine-tomography as a 4D imaging technique developed to study real-time dynamics in small living organisms with micrometer spatial resolution and subsecond time resolution. The method enables insights into the physiology of small animals by tracking the 4D morphological dynamics of minute anatomical features as demonstrated in this work by the analysis of fast-moving screw-and-nut–type weevil hip joints. The presented method can be applied to a broad range of biological specimens and biotechnological processes.
@article{dosSantosRolo2014,
abstract = {Scientific cinematography using ultrafast optical imaging is a common tool to study motion. In opaque organisms or structures, X-ray radiography captures sequences of 2D projections to visualize morphological dynamics, but for many applications full four-dimensional (4D) spatiotemporal information is highly desirable. We introduce in vivo X-ray cine-tomography as a 4D imaging technique developed to study real-time dynamics in small living organisms with micrometer spatial resolution and subsecond time resolution. The method enables insights into the physiology of small animals by tracking the 4D morphological dynamics of minute anatomical features as demonstrated in this work by the analysis of fast-moving screw-and-nut–type weevil hip joints. The presented method can be applied to a broad range of biological specimens and biotechnological processes.},
added-at = {2014-11-25T14:02:20.000+0100},
author = {dos Santos Rolo, Tomy and Ershov, Alexey and van de Kamp, Thomas and Baumbach, Tilo},
biburl = {https://www.bibsonomy.org/bibtex/2a922c5e5ffcea538f996d63d8b662d4d/tomyrolo},
doi = {10.1073/pnas.1308650111},
eprint = {http://www.pnas.org/content/111/11/3921.full.pdf+html},
interhash = {5487e7a94ccda48ffbf683a2015fe96e},
intrahash = {a922c5e5ffcea538f996d63d8b662d4d},
journal = {Proceedings of the National Academy of Sciences},
keywords = {cine-tomography in-vivo_imaging morphological_dynanics motion_tracking myown screw_joint x-ray},
number = 11,
pages = {3921-3926},
timestamp = {2014-11-25T14:02:20.000+0100},
title = {In vivo X-ray cine-tomography for tracking morphological dynamics},
url = {http://www.pnas.org/content/111/11/3921.abstract},
volume = 111,
year = 2014
}