@article{citeulike:489707,
title = {Development of a radial immunodiffusion technique employing monoclonal antibodies for apolipoprotein B determination in human plasma.},
author = {S. Marcovina and G. Di Cola and C. Rapetto and C. Fievet and V. Clavey and J. C. Fruchart},
journal = {Clinica Chimica Acta},
month = {April},
number = {2},
pages = {117--125},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=3921291},
volume = {147},
year = {1985},
abstract = {Twenty monoclonal antibodies obtained from two different fusions of SP2/0-Ag 14 cell line (non-secretor hybridoma) with human plasma low density lipoproteins have been selected. We found that a mixture formed by the 20 monoclonal antibodies was able to form a single precipitin line with human plasma low density lipoproteins by a double gel diffusion technique. Further studies revealed that only two monoclonal antibodies were needed to precipitate low density lipoproteins in gel. However, a minimum of four particular monoclonal antibodies was required to obtain an optimal precipitin ring and a linear standard curve within 24 h using a radial immunodiffusion technique. We have then compared the radial immunodiffusion performed with a monoclonal antibody mixture to those employing conventional goat and rabbit antibodies in terms of plasma apolipoprotein B determinations. The apolipoprotein B values determined by monoclonal antibodies significantly correlate with the values obtained by the technique using goat (r = 0.95; p less than 0.001) and rabbit (r = 0.95; p less than 0.001) antibodies. Our data indicate that a mixture of monoclonal antibodies can mimic conventional antibodies in terms of immunoprecipitation and apolipoprotein B determination.},
issn = {0009-8981}, doi = {10.1016/0009-8981(85)90072-5 }, citeulike-article-id = {489707}, priority = {2},
keywords = {antibody apob immunoassay }
}
::