%0 Journal Article %1 valenza_evaluation_2011 %A Valenza, G %A Müller, S %A Schmitt, C %A Turnwald, D %A Lam, T-T %A Frosch, M %A Abele-Horn, M %A Pfeifer, Y %D 2011 %J European Journal of Clinical Microbiology & Infectious Diseases: Official Publication of the European Society of Clinical Microbiology %K ag_horn imported %R 10.1007/s10096-011-1169-2 %T Evaluation of the VITEK 2 AST-N111 card for detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca compared to ESBL Etests and combination disk methods %U http://www.ncbi.nlm.nih.gov/pubmed/21271268 %X The VITEK 2 AST-N111 card was evaluated for detection of extended-spectrum beta-lactamases (ESBLs) by testing 51 ESBL positive and 50 ESBL negative isolates of E. coli, K. pneumoniae, and K. oxytoca. The occurrence of beta-lactamase genes was confirmed by PCR and sequencing. The advanced expert system (AES) of the VITEK 2 system achieved sensitivity and specificity values of 100\% and 96.0\%, respectively. The ESBL test of the VITEK 2 AST-N111 card showed a sensitivity of 92.1\% and a specificity of 90.0\%. Contradictory results obtained with the two VITEK 2 tools could be clarified by combination disk tests in nine of 11 isolates. The combined use of AES and ESBL tests of the AST-N111 card in association with combination disk tests in case of contradictory results seems to be a reliable method for ESBL detection.

@article{valenza_evaluation_2011, abstract = {The {VITEK} 2 {AST-N111} card was evaluated for detection of extended-spectrum beta-lactamases {(ESBLs)} by testing 51 {ESBL} positive and 50 {ESBL} negative isolates of E. coli, K. pneumoniae, and K. oxytoca. The occurrence of beta-lactamase genes was confirmed by {PCR} and sequencing. The advanced expert system {(AES)} of the {VITEK} 2 system achieved sensitivity and specificity values of 100\% and 96.0\%, respectively. The {ESBL} test of the {VITEK} 2 {AST-N111} card showed a sensitivity of 92.1\% and a specificity of 90.0\%. Contradictory results obtained with the two {VITEK} 2 tools could be clarified by combination disk tests in nine of 11 isolates. The combined use of {AES} and {ESBL} tests of the {AST-N111} card in association with combination disk tests in case of contradictory results seems to be a reliable method for {ESBL} detection.}, added-at = {2011-04-07T15:44:20.000+0200}, author = {Valenza, G and Müller, S and Schmitt, C and Turnwald, D and Lam, {T-T} and Frosch, M and {Abele-Horn}, M and Pfeifer, Y}, biburl = {https://www.bibsonomy.org/bibtex/21db7b1f5f3d1f9a323d162deb42ab86a/hymi}, doi = {10.1007/s10096-011-1169-2}, interhash = {3132789c7918f32864b1f2cc4640ba3c}, intrahash = {1db7b1f5f3d1f9a323d162deb42ab86a}, issn = {1435-4373}, journal = {European Journal of Clinical Microbiology \& Infectious Diseases: Official Publication of the European Society of Clinical Microbiology}, keywords = {ag_horn imported}, month = jan, note = {{PMID:} 21271268}, timestamp = {2011-04-07T15:47:41.000+0200}, title = {Evaluation of the {VITEK} 2 {AST-N111} card for detection of extended-spectrum beta-lactamases {(ESBLs)} in Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca compared to {ESBL} Etests and combination disk methods}, url = {http://www.ncbi.nlm.nih.gov/pubmed/21271268}, year = 2011 }