%0 Journal Article %1 Rieg.2013 %A Rieg, Siegbert %A Jonas, Daniel %A Kaasch, Achim J. %A Porzelius, Christine %A Peyerl-Hoffmann, Gabriele %A Theilacker, Christian %A Küpper, Marc-Fabian %A Schneider, Christian %A Seifert, Harald %A Kern, Winfried V. %D 2013 %J PloS one %K Aged Bacteremia/diagnosis/microbiology Blood_Vessels/microbiology Bone_Diseases,_Infectious/diagnosis/microbiology Cohort_Studies Endocarditis,_Bacterial/diagnosis/microbiology Female Genotyping_Techniques Humans Joint_Diseases/microbiology Male Oligonucleotide_Array_Sequence_Analysis Prognosis Prospective_Studies Staphylococcal_Infections/diagnosis/microbiology Staphylococcus_aureus/genetics/growth_&_development/physiology Tropism/genetics %N 8 %P e71259 %T Microarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study %V 8 %X The clinical course of Staphylococcus aureus bacteremia varies extensively. We sought to determine the relationship between genetic characteristics of the infecting pathogen and clinical outcomes in an exploratory study. In two study centers, 317 blood culture isolates were analyzed by DNA microarray and spa genotyping. By uni- and multivariate regression analyses associations of genotype data with 30-day all-cause mortality, severe sepsis/septic shock, disseminated disease, endocarditis, and osteoarticular infection were investigated. Univariate analysis showed significant association between S. aureus genes/gene-clusters or clonal complexes and clinical endpoints. For example CC15 was associated with 30-day mortality and CC22 with osteoarticular infection. In multivariate analysis methicillin resistance (mecA, OR 4.8 1.43-16.06) and the beta-lactamase-gene (bla, OR 3.12 1.17-8.30) remained independently associated with 30-day mortality. The presence of genes for enterotoxins (sed/sej/ser) was associated with endocarditis (OR 5.11 1.14-18.62). Host factors such as McCabe classification (OR 4.52 2.09-9.79 for mortality), age (OR 1.06 1.03-1.10 per year), and community-acquisition (OR 3.40 1.31-8.81) had a major influence on disease severity, dissemination and mortality. Individual genotypes and clonal complexes of S. aureus can only partially explain clinical features and outcomes of S. aureus bacteremia. Genotype-phenotype association studies need to include adjustments for host factors like age, comorbidity and community-acquisition.

@article{Rieg.2013, abstract = {The clinical course of Staphylococcus aureus bacteremia varies extensively. We sought to determine the relationship between genetic characteristics of the infecting pathogen and clinical outcomes in an exploratory study. In two study centers, 317 blood culture isolates were analyzed by DNA microarray and spa genotyping. By uni- and multivariate regression analyses associations of genotype data with 30-day all-cause mortality, severe sepsis/septic shock, disseminated disease, endocarditis, and osteoarticular infection were investigated. Univariate analysis showed significant association between S. aureus genes/gene-clusters or clonal complexes and clinical endpoints. For example CC15 was associated with 30-day mortality and CC22 with osteoarticular infection. In multivariate analysis methicillin resistance (mecA, OR 4.8 [1.43-16.06]) and the beta-lactamase-gene (bla, OR 3.12 [1.17-8.30]) remained independently associated with 30-day mortality. The presence of genes for enterotoxins (sed/sej/ser) was associated with endocarditis (OR 5.11 [1.14-18.62]). Host factors such as McCabe classification (OR 4.52 [2.09-9.79] for mortality), age (OR 1.06 [1.03-1.10] per year), and community-acquisition (OR 3.40 [1.31-8.81]) had a major influence on disease severity, dissemination and mortality. Individual genotypes and clonal complexes of S. aureus can only partially explain clinical features and outcomes of S. aureus bacteremia. Genotype-phenotype association studies need to include adjustments for host factors like age, comorbidity and community-acquisition.}, added-at = {2014-10-13T18:24:58.000+0200}, author = {Rieg, Siegbert and Jonas, Daniel and Kaasch, Achim J. and Porzelius, Christine and Peyerl-Hoffmann, Gabriele and Theilacker, Christian and Küpper, Marc-Fabian and Schneider, Christian and Seifert, Harald and Kern, Winfried V.}, biburl = {https://www.bibsonomy.org/bibtex/2dbb703553d4dbdc47084fe81f03952a3/drtester}, interhash = {39d7563e4ebc5ef362c82364c05af773}, intrahash = {dbb703553d4dbdc47084fe81f03952a3}, journal = {PloS one}, keywords = {Aged Bacteremia/diagnosis/microbiology Blood_Vessels/microbiology Bone_Diseases,_Infectious/diagnosis/microbiology Cohort_Studies Endocarditis,_Bacterial/diagnosis/microbiology Female Genotyping_Techniques Humans Joint_Diseases/microbiology Male Oligonucleotide_Array_Sequence_Analysis Prognosis Prospective_Studies Staphylococcal_Infections/diagnosis/microbiology Staphylococcus_aureus/genetics/growth_&_development/physiology Tropism/genetics}, number = 8, pages = {e71259}, timestamp = {2014-10-13T18:24:58.000+0200}, title = {Microarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study}, volume = 8, year = 2013 }