%0 Journal Article %1 findlow_three_2007 %A Findlow, Helen %A Vogel, Ulrich %A Mueller, Judith E %A Curry, Alan %A Njanpop-Lafourcade, Berthe-Marie %A Claus, Heike %A Gray, Stephen J %A Yaro, Seydou %A Traoré, Yves %A Sangaré, Lassana %A Nicolas, Pierre %A Gessner, Bradford D %A Borrow, Ray %D 2007 %J The Journal of Infectious Diseases %K Adolescent, Assay, Bacterial Bacterial, Capsules, Carrier Cerebrospinal Chain Child, Diagnosis, Differential, Female, Fluid, Humans, Immunosorbent Infections, Lipopolysaccharides, Male, Meningococcal Neisseria Polymerase Reaction State, meningitidis, {DNA,} {Enzyme-Linked} %N 7 %P 1071--1077 %R 10.1086/512084 %T Three cases of invasive meningococcal disease caused by a capsule null locus strain circulating among healthy carriers in Burkina Faso %U http://www.ncbi.nlm.nih.gov/pubmed/17330799 %V 195 %X During reinforced surveillance of acute bacterial meningitis in Burkina Faso, meningococcal strains of phenotype NG:NT:NST were isolated from cerebrospinal fluid samples from 3 patients. The strains were negative for the ctrA gene but were positive for the crgA gene. Molecular typing revealed that the strains harbored the capsule null locus (cnl) and belonged to the multilocus sequence type (ST)-192. PorA sequencing showed that all strains were either P1.18-11,42; P1.18,42-1; P1.18-11,42-1; P1.18-11,42-3; or P1.18-12,42-1. Sequencing also showed that all strains were negative for the FetA receptor gene. Serum killing assays showed these strains to be resistant, with the resistance comparable with that of a fully capsular serogroup B strain, MC58. The same strains were found in 14 healthy carriers in the general population of Bobo-Dioulasso (100\% of ST-192 isolates tested for cnl). The presence of cnl meningococci that can escape serum killing and cause invasive disease is of concern for future vaccination strategies and should promote rigorous surveillance of cnl meningococcal disease.

@article{findlow_three_2007, abstract = {During reinforced surveillance of acute bacterial meningitis in Burkina Faso, meningococcal strains of phenotype {NG:NT:NST} were isolated from cerebrospinal fluid samples from 3 patients. The strains were negative for the {ctrA} gene but were positive for the {crgA} gene. Molecular typing revealed that the strains harbored the capsule null locus (cnl) and belonged to the multilocus sequence type {(ST)-192.} {PorA} sequencing showed that all strains were either P1.18-11,42; P1.18,42-1; P1.18-11,42-1; P1.18-11,42-3; or P1.18-12,42-1. Sequencing also showed that all strains were negative for the {FetA} receptor gene. Serum killing assays showed these strains to be resistant, with the resistance comparable with that of a fully capsular serogroup B strain, {MC58.} The same strains were found in 14 healthy carriers in the general population of {Bobo-Dioulasso} (100\% of {ST-192} isolates tested for cnl). The presence of cnl meningococci that can escape serum killing and cause invasive disease is of concern for future vaccination strategies and should promote rigorous surveillance of cnl meningococcal disease.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Findlow, Helen and Vogel, Ulrich and Mueller, Judith E and Curry, Alan and {Njanpop-Lafourcade}, {Berthe-Marie} and Claus, Heike and Gray, Stephen J and Yaro, Seydou and Traoré, Yves and Sangaré, Lassana and Nicolas, Pierre and Gessner, Bradford D and Borrow, Ray}, biburl = {https://www.bibsonomy.org/bibtex/20996a669b784ca3068381b09dc975a59/jelias}, doi = {10.1086/512084}, interhash = {79b9fb9f3f0ac888e768ce7541b83afe}, intrahash = {0996a669b784ca3068381b09dc975a59}, issn = {0022-1899}, journal = {The Journal of Infectious Diseases}, keywords = {Adolescent, Assay, Bacterial Bacterial, Capsules, Carrier Cerebrospinal Chain Child, Diagnosis, Differential, Female, Fluid, Humans, Immunosorbent Infections, Lipopolysaccharides, Male, Meningococcal Neisseria Polymerase Reaction State, meningitidis, {DNA,} {Enzyme-Linked}}, month = apr, note = {{PMID:} 17330799}, number = 7, pages = {1071--1077}, timestamp = {2011-03-11T10:06:35.000+0100}, title = {Three cases of invasive meningococcal disease caused by a capsule null locus strain circulating among healthy carriers in Burkina Faso}, url = {http://www.ncbi.nlm.nih.gov/pubmed/17330799}, volume = 195, year = 2007 }