%0 Conference Paper %1 5333756 %A Pastor, D. %A Luengo-Oroz, M. A. %A Lombardot, B. %A Gonzalvez, I. %A Duloquin, L. %A Savy, T. %A Bourgine, P. %A Peyrieras, N. %A Santos, A. %B Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE %D 2009 %K biology cells development embryos morphology tracking %P 970-973 %R 10.1109/IEMBS.2009.5333756 %T Cell tracking in fluorescence images of embryogenesis processes with morphological reconstruction by 4D-tubular structuring elements %U http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=5333756&tag=1 %X We present a simple and parameter-free nuclei tracking method for reconstructing cell dynamics in fluorescence 3D+t images of embryogenesis. The strategy is based on the use of the mathematical morphology operators directly in the 4D image. The morphological reconstruction of a marker -manually or automatically selected- in an initial spatio-temporal position generates a connected path over the time representing the cell migration. Thus, the processing provides a coherent spatiotemporal estimation of cell movement. The algorithm has been validated on in vivo images of early zebrafish and sea urchin embryogenesis acquired with two-photon laser scanning microscopy providing mean tracking rates above 98% per time step.

@inproceedings{5333756, abstract = {We present a simple and parameter-free nuclei tracking method for reconstructing cell dynamics in fluorescence 3D+t images of embryogenesis. The strategy is based on the use of the mathematical morphology operators directly in the 4D image. The morphological reconstruction of a marker -manually or automatically selected- in an initial spatio-temporal position generates a connected path over the time representing the cell migration. Thus, the processing provides a coherent spatiotemporal estimation of cell movement. The algorithm has been validated on in vivo images of early zebrafish and sea urchin embryogenesis acquired with two-photon laser scanning microscopy providing mean tracking rates above 98% per time step.}, added-at = {2013-10-24T14:17:30.000+0200}, author = {Pastor, D. and Luengo-Oroz, M. A. and Lombardot, B. and Gonzalvez, I. and Duloquin, L. and Savy, T. and Bourgine, P. and Peyrieras, N. and Santos, A.}, biburl = {https://www.bibsonomy.org/bibtex/283fcb29eb12de6738437fcfb23f6cbc0/alex_ruff}, booktitle = {Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE}, description = {IEEE Xplore - Cell tracking in fluorescence images of embryogenesis processes with morphological reconstruction by...}, doi = {10.1109/IEMBS.2009.5333756}, interhash = {8329ff27f42400cf51fa61115893671e}, intrahash = {83fcb29eb12de6738437fcfb23f6cbc0}, issn = {1557-170X}, keywords = {biology cells development embryos morphology tracking}, pages = {970-973}, timestamp = {2013-10-24T14:17:30.000+0200}, title = {Cell tracking in fluorescence images of embryogenesis processes with morphological reconstruction by 4D-tubular structuring elements}, url = {http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=5333756&tag=1}, year = 2009 }