%0 Journal Article %1 lujambio2007genetic %A Lujambio, Amaia %A Ropero, Santiago %A Ballestar, Esteban %A Fraga, Mario F %A Cerrato, Celia %A Setién, Fernando %A Casado, Sara %A Suarez-Gauthier, Ana %A Sanchez-Cespedes, Montserrat %A Git, Anna %A Spiteri, Immaculada %A Das, Partha P %A Caldas, Carlos %A Miska, Eric %A Esteller, Manel %D 2007 %J Cancer Research %K 7 background cancer methylation mirnas %N 4 %P 1424-1429 %R 10.1158/0008-5472.CAN-06-4218 %T Genetic Unmasking of an Epigenetically Silenced microRNA in Human Cancer Cells %U http://cancerres.aacrjournals.org/content/67/4/1424.long %V 67 %X The mechanisms underlying microRNA (miRNA) disruption in human disease are poorly understood. In cancer cells, the transcriptional silencing of tumor suppressor genes by CpG island promoter hypermethylation has emerged as a common hallmark. We wondered if the same epigenetic disruption can “hit” miRNAs in transformed cells. To address this issue, we have used cancer cells genetically deficient for the DNA methyltransferase enzymes in combination with a miRNA expression profiling. We have observed that DNA hypomethylation induces a release of miRNA silencing in cancer cells. One of the main targets is miRNA-124a, which undergoes transcriptional inactivation by CpG island hypermethylation in human tumors from different cell types. Interestingly, we functionally link the epigenetic loss of miRNA-124a with the activation of cyclin D kinase 6, a bona fide oncogenic factor, and the phosphorylation of the retinoblastoma, a tumor suppressor gene. Cancer Res 2007;67(4):1424–9

@article{lujambio2007genetic, abstract = {The mechanisms underlying microRNA (miRNA) disruption in human disease are poorly understood. In cancer cells, the transcriptional silencing of tumor suppressor genes by CpG island promoter hypermethylation has emerged as a common hallmark. We wondered if the same epigenetic disruption can “hit” miRNAs in transformed cells. To address this issue, we have used cancer cells genetically deficient for the DNA methyltransferase enzymes in combination with a miRNA expression profiling. We have observed that DNA hypomethylation induces a release of miRNA silencing in cancer cells. One of the main targets is miRNA-124a, which undergoes transcriptional inactivation by CpG island hypermethylation in human tumors from different cell types. Interestingly, we functionally link the epigenetic loss of miRNA-124a with the activation of cyclin D kinase 6, a bona fide oncogenic factor, and the phosphorylation of the retinoblastoma, a tumor suppressor gene. [Cancer Res 2007;67(4):1424–9]}, added-at = {2017-10-19T19:55:48.000+0200}, author = {Lujambio, Amaia and Ropero, Santiago and Ballestar, Esteban and Fraga, Mario F and Cerrato, Celia and Setién, Fernando and Casado, Sara and Suarez-Gauthier, Ana and Sanchez-Cespedes, Montserrat and Git, Anna and Spiteri, Immaculada and Das, Partha P and Caldas, Carlos and Miska, Eric and Esteller, Manel}, biburl = {https://www.bibsonomy.org/bibtex/26b66e806243b02dd8e3239e10fc7acf9/artheibault}, description = {This article describes methylation of the miR-124 promoter region in cancer.}, doi = {10.1158/0008-5472.CAN-06-4218}, interhash = {84f4f58ed3c6eea4af3d8f160da4ec9c}, intrahash = {6b66e806243b02dd8e3239e10fc7acf9}, journal = {Cancer Research}, keywords = {7 background cancer methylation mirnas}, month = feb, number = 4, pages = {1424-1429}, timestamp = {2017-10-25T21:38:29.000+0200}, title = {Genetic Unmasking of an Epigenetically Silenced microRNA in Human Cancer Cells}, url = {http://cancerres.aacrjournals.org/content/67/4/1424.long}, volume = 67, year = 2007 }