%0 Journal Article %1 Vissers.1988 %A Vissers, M. C. %A Jester, S. A. %A Fantone, J. C. %D 1988 %J J.Immunol.Methods %K Cell Centrifugation Colloids Count Density Diatrizoate Dioxide Ficoll Gradient Human Humans Leukocyte Lymphocytes Monocytes Polysaccharides Population Separation Silicon blood cells methods pathology %N 2 %P 203-207 %T Rapid purification of human peripheral blood monocytes by centrifugation through Ficoll-Hypaque and Sepracell-MN %U PM:2837516 %V 110 %X We have developed a rapid and simple method for isolating human peripheral blood monocytes in suspension. The procedure combines two separation media and involves isolation of the mononuclear cells by centrifugation through Ficoll-Hypaque followed by purification of the monocytes using Sepracell-MN, a colloidal silica-based medium. The final cell population contained approximately 90% monocytes with good functional ability. The contaminating cells were lymphocytes. Viability was always greater than or equal to 99% with 90% recovery of the monocytes from the mononuclear cells

@article{Vissers.1988, abstract = {We have developed a rapid and simple method for isolating human peripheral blood monocytes in suspension. The procedure combines two separation media and involves isolation of the mononuclear cells by centrifugation through Ficoll-Hypaque followed by purification of the monocytes using Sepracell-MN, a colloidal silica-based medium. The final cell population contained approximately 90% monocytes with good functional ability. The contaminating cells were lymphocytes. Viability was always greater than or equal to 99% with 90% recovery of the monocytes from the mononuclear cells}, added-at = {2010-02-05T11:28:39.000+0100}, author = {Vissers, M. C. and Jester, S. A. and Fantone, J. C.}, biburl = {https://www.bibsonomy.org/bibtex/20ee2eb582439dba0e179ea92562049c3/kanefendt}, interhash = {8f81a11bc5b1b9b0b095a5501678b655}, intrahash = {0ee2eb582439dba0e179ea92562049c3}, journal = {J.Immunol.Methods}, keywords = {Cell Centrifugation Colloids Count Density Diatrizoate Dioxide Ficoll Gradient Human Humans Leukocyte Lymphocytes Monocytes Polysaccharides Population Separation Silicon blood cells methods pathology}, number = 2, pages = {203-207}, timestamp = {2010-02-05T11:28:44.000+0100}, title = {Rapid purification of human peripheral blood monocytes by centrifugation through Ficoll-Hypaque and Sepracell-MN}, url = {PM:2837516}, volume = 110, year = 1988 }