%0 Journal Article %1 Silva2011 %A Silva, Eriques Gonçalves %A Paula, Claudete Rodrigues %A Dias, Amanda Latercia Tranches %A Chang, Marilene Rodrigues %A Ruiz, Luciana da Silva %A Gambale, Valderez %A Prates, Renato Araujo %A Ribeiro, Martha Simões %D 2011 %J Mycopathologia %K fungal dosing murine drug %N 4 %P 261--266 %R 10.1007/s11046-010-9375-5 %T Combination efficacy of voriconazole and amphotericin B in the experimental disease in immunodeficient mice caused by fluconazole-resistant Cryptococcus neoformans. %U http://dx.doi.org/10.1007/s11046-010-9375-5 %V 171 %X The therapeutic efficacy of amphotericin B and voriconazole alone and in combination with one another were evaluated in immunodeficient mice (BALB/c-SCID) infected with a fluconazole-resistant strain of Cryptococcus neoformans var. grubii. The animals were infected intravenously with 3 × 10(5) cells and intraperitoneally treated with amphotericin B (1.5 mg/kg/day) in combination with voriconazole (40 mg/kg/days). Treatment began 1 day after inoculation and continued for 7 and 15 days post-inoculation. The treatments were evaluated by survival curves and yeast quantification (CFUs) in brain and lung tissues. Treatments for 15 days significantly promoted the survival of the animals compared to the control groups. Our results indicated that amphotericin B was effective in assuring longest-term survival of infected animals, but these animals still harbored the highest CFU of C. neoformans in lungs and brain at the end of the experiment. Voriconazole was not as effective alone, but in combination with amphotericin B, it prolonged survival for the second-longest time period and provided the lowest colonization of target organs by the fungus. None of the treatments were effective in complete eradication of the fungus in mice lungs and brain at the end of the experiment.

@article{Silva2011, abstract = {The therapeutic efficacy of amphotericin B and voriconazole alone and in combination with one another were evaluated in immunodeficient mice (BALB/c-SCID) infected with a fluconazole-resistant strain of Cryptococcus neoformans var. grubii. The animals were infected intravenously with 3 × 10(5) cells and intraperitoneally treated with amphotericin B (1.5 mg/kg/day) in combination with voriconazole (40 mg/kg/days). Treatment began 1 day after inoculation and continued for 7 and 15 days post-inoculation. The treatments were evaluated by survival curves and yeast quantification (CFUs) in brain and lung tissues. Treatments for 15 days significantly promoted the survival of the animals compared to the control groups. Our results indicated that amphotericin B was effective in assuring longest-term survival of infected animals, but these animals still harbored the highest CFU of C. neoformans in lungs and brain at the end of the experiment. Voriconazole was not as effective alone, but in combination with amphotericin B, it prolonged survival for the second-longest time period and provided the lowest colonization of target organs by the fungus. None of the treatments were effective in complete eradication of the fungus in mice lungs and brain at the end of the experiment.}, added-at = {2013-03-30T01:48:23.000+0100}, author = {Silva, Eriques Gonçalves and Paula, Claudete Rodrigues and Dias, Amanda Latercia Tranches and Chang, Marilene Rodrigues and Ruiz, Luciana da Silva and Gambale, Valderez and Prates, Renato Araujo and Ribeiro, Martha Simões}, biburl = {https://www.bibsonomy.org/bibtex/2139b6f07eb3ce111fc8616d0d3d18181/aorchid}, description = {see page 91 notebook 2. Neither vori in combo completely erradicated in model, but combo was better.}, doi = {10.1007/s11046-010-9375-5}, file = {:ID_General/Fungus_most/Mycopathologia.171.261.pdf:PDF}, groups = {public}, institution = {Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Professor Lineu Prestes 1374, 2º andar, sala 247, São Paulo (USP), 05508-900, Brazil. eriques@usp.br}, interhash = {96753fb77e49fd9189c1d4069216b4f2}, intrahash = {139b6f07eb3ce111fc8616d0d3d18181}, journal = {Mycopathologia}, keywords = {fungal dosing murine drug}, language = {eng}, medline-pst = {ppublish}, month = Apr, number = 4, pages = {261--266}, pmid = {20972836}, timestamp = {2013-03-30T01:52:04.000+0100}, title = {Combination efficacy of voriconazole and amphotericin B in the experimental disease in immunodeficient mice caused by fluconazole-resistant Cryptococcus neoformans.}, url = {http://dx.doi.org/10.1007/s11046-010-9375-5}, username = {aorchid}, volume = 171, year = 2011 }