Abstract
A high-performance liquid chromatographic method with photodiode array detection has been developed for the determination of squalene. After treated by extraction and fractional crystallization, squalene was analyzed on a C-18 column (150 x 3.9 mm, 5 mum) with acetonitrile as mobile phase. Excellent linearity of the calibration curve was observed in the range of 10040000 mugL(-1) and the detection limit was 40 mugL(-1). The recoveries were from 89.6% to 100.5% and the relative standard deviations were from 0.5% to 1.4%. The method was successfully applied to the determination of squalene in squalene capsules, olive oil, algal lipids and algal cells.
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