Article,
Polymer encapsulated dopaminergic cell lines as älternative neural grafts".
Prog Brain Res, (1990)
Abstract
Our preliminary findings (Jaeger et al., 1988; Aebischer et al., 1989;
Tresco et al., 1989) and the studies in progress show that encapsulated
dopaminergic cell lines survive enclosure within a semi-permeable
membrane. The encapsulated cells remained viable for extended time
periods when maintained in vitro. Moreover, encapsulated PC12 and
T28 cells have the potential to survive following their implantation
into the forebrain of rats. Cell lines are essentially "immortal"
because they continue to divide indefinitely. This property allows
perpetual "self-renewal" of a given cell population. However, the
capacity of continuous uncontrolled cell division may also lead to
tumor formation. This in fact is the case for unencapsulated PC12
cell implants placed into the brain of young Sprague Dawley rats
(Jaeger, 1985). Cell line encapsulation has the potential to prevent
tumor growth (Jaeger et al., 1988). Survival for 6 months in vitro
suggests that encapsulation does not preclude long-term maintenance
of an homogeneous cell line like PC12 cells. The presence of mitotic
figures in the capsules further supports the likelihood of propagation
and self renewal of the encapsulated population. Another significant
property of cell lines is that they consist of a single, genetically
homogeneous cell type. They do not require specific synaptic interactions
for their survival. In the case of PC12 and T28 lines, the cells
synthesize and release neurotransmitters. Our data show that PC12
and T28 cells continue to release dopamine spontaneously and to express
specific transmitters and enzymes following encapsulation. Thus,
cell lines such as these may constitute relatively simple "neural
implants" exerting their function via humoral release.(ABSTRACT TRUNCATED
AT 250 WORDS)
Tags
- *brain
- *prostheses
- *transplantation,
- ;
- adrenal
- and
- animals
- artificial
- cell
- cells,
- compounding
- cultured/*transplantation
- disease/*surgery
- division
- dopamine/*secretion
- drug
- gland
- gov't,
- graft
- heterotopic
- implants
- line
- membranes,
- mice
- neoplasms/pathology
- neuroblastoma/pathology
- p.h.s.
- parkinson
- pheochromocytoma/pathology
- rats
- research
- support,
- survival
- tumor
- u.s.
