Abstract
A PCR-based assay for Bordetella pertussis was inhibited by using a calcium alginate fiber-tipped swab with an aluminum shaft but not by using a Dacron fiber-tipped swab with a plastic shaft. The calcium alginate fiber component inhibited the assay following storage for less than 1 min in a suspension of 10(3) CFU of B. pertussis per ml, whereas the aluminum shaft component required storage for at least 48 h in order to cause inhibition. We recommend the Dacron swab over the calcium alginate swab for collecting specimens for testing in PCR-based assays.
- acid,
- acids,
- alginates,
- aluminum,
- bacterial,
- bacteriological
- base
- bordetella
- chain
- cough
- data,
- glucuronic
- hexuronic
- humans,
- imported
- molecular
- nasopharynx,
- pertussis,
- polyethylene
- polymerase
- probes,
- reaction,
- sequence
- sequence,
- techniques,
- terephthalates,
- whooping
- {dna,}
- {dna}
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