%0 Journal Article %1 yim2005nanopatterninduced %A Yim, Evelyn K.F. %A Reano, Ron M. %A Pang, Stella W. %A Yee, Albert F. %A Chen, Christopher S. %A Leong, Kam W. %D 2005 %J Biomaterials %K biomaterial nanotopography phd %N 26 %P 5405--5413 %R http://dx.doi.org/10.1016/j.biomaterials.2005.01.058 %T Nanopattern-induced changes in morphology and motility of smooth muscle cells %U http://www.sciencedirect.com/science/article/pii/S0142961205001080 %V 26 %X Cells are known to be surrounded by nanoscale topography in their natural extracellular environment. The cell behavior, including morphology, proliferation, and motility of bovine pulmonary artery smooth muscle cells (SMC) were studied on poly(methyl methacrylate) (PMMA) and poly(dimethylsiloxane) (PDMS) surfaces comprising nanopatterned gratings with 350 nm linewidth, 700 nm pitch, and 350 nm depth. More than 90% of the cells aligned to the gratings, and were significantly elongated compared to the \SMC\ cultured on non-patterned surfaces. The nuclei were also elongated and aligned. Proliferation of the cells was significantly reduced on the nanopatterned surfaces. The polarization of microtubule organizing centers (MTOC), which are associated with cell migration, of \SMC\ cultured on nanopatterned surfaces showed a preference towards the axis of cell alignment in an in vitro wound healing assay. In contrast, the \MTOC\ of \SMC\ on non-patterned surfaces preferentially polarized towards the wound edge. It is proposed that this nanoimprinting technology will provide a valuable platform for studies in cell-substrate interactions and for development of medical devices with nanoscale features.

@article{yim2005nanopatterninduced, abstract = {Cells are known to be surrounded by nanoscale topography in their natural extracellular environment. The cell behavior, including morphology, proliferation, and motility of bovine pulmonary artery smooth muscle cells (SMC) were studied on poly(methyl methacrylate) (PMMA) and poly(dimethylsiloxane) (PDMS) surfaces comprising nanopatterned gratings with 350 nm linewidth, 700 nm pitch, and 350 nm depth. More than 90% of the cells aligned to the gratings, and were significantly elongated compared to the \{SMC\} cultured on non-patterned surfaces. The nuclei were also elongated and aligned. Proliferation of the cells was significantly reduced on the nanopatterned surfaces. The polarization of microtubule organizing centers (MTOC), which are associated with cell migration, of \{SMC\} cultured on nanopatterned surfaces showed a preference towards the axis of cell alignment in an in vitro wound healing assay. In contrast, the \{MTOC\} of \{SMC\} on non-patterned surfaces preferentially polarized towards the wound edge. It is proposed that this nanoimprinting technology will provide a valuable platform for studies in cell-substrate interactions and for development of medical devices with nanoscale features. }, added-at = {2015-07-28T11:28:47.000+0200}, author = {Yim, Evelyn K.F. and Reano, Ron M. and Pang, Stella W. and Yee, Albert F. and Chen, Christopher S. and Leong, Kam W.}, biburl = {https://www.bibsonomy.org/bibtex/20aed2affdee5b6fea2def760b24499e4/bkoch}, description = {Nanopattern-induced changes in morphology and motility of smooth muscle cells}, doi = {http://dx.doi.org/10.1016/j.biomaterials.2005.01.058}, interhash = {e651112a72590538a6a7b6598db36984}, intrahash = {0aed2affdee5b6fea2def760b24499e4}, issn = {0142-9612}, journal = {Biomaterials }, keywords = {biomaterial nanotopography phd}, number = 26, pages = {5405--5413}, timestamp = {2015-07-28T11:28:47.000+0200}, title = {Nanopattern-induced changes in morphology and motility of smooth muscle cells }, url = {http://www.sciencedirect.com/science/article/pii/S0142961205001080}, volume = 26, year = 2005 }