]> BibSonomy publications for /author/Gomelsky/Base_Sequence http://www.bibsonomy.org/burst/author/Gomelsky/Base_Sequence BibSonomy BuRST Feed for /author/Gomelsky/Base_Sequence 2008-11-19T05:55:48+01:00 Regulation of hydrogen peroxide-dependent gene expression in Rhodobacter sphaeroides: regulatory functions of OxyR http://www.bibsonomy.org/bibtex/28f70cb140c45721ef0acab94f03009d2/mikromolbio mikromolbio 2008-05-07T15:52:44+02:00 Bacterial Base_Sequence Consensus_Sequence Gene_Expression_Regulation Genetic Hydrogen_Peroxide IFZ Molecular_Sequence_Data Mutation Oxidants Regulon Rhodobacter_sphaeroides Transcription Transcription_Factors
Regulation of hydrogen peroxide-dependent gene expression in Rhodobacter sphaeroides: regulatory functions of OxyR
Tanja Zeller and Mobarak A Mraheil and Oleg V Moskvin and Kuanyu Li and Mark Gomelsky and Gabriele Klug Journal of bacteriology 189 3784-92 (2007)
to Bacterial Base_Sequence Consensus_Sequence Gene_Expression_Regulation Genetic Hydrogen_Peroxide IFZ Molecular_Sequence_Data Mutation Oxidants Regulon Rhodobacter_sphaeroides Transcription Transcription_Factors by mikromolbio on 2008-05-07 15:52:44 ]]> Journal of bacteriologyMayPMID: 173510373784-92Regulation of hydrogen peroxide-dependent gene expression in Rhodobacter sphaeroides: regulatory functions of OxyR1892007Bacterial Base_Sequence Consensus_Sequence Gene_Expression_Regulation Genetic Hydrogen_Peroxide IFZ Molecular_Sequence_Data Mutation Oxidants Regulon Rhodobacter_sphaeroides Transcription Transcription_Factors 2008-05-07 15:52:44.0Genome-wide transcriptome profiling was used to reveal hydrogen peroxide (H(2)O(2))-dependent regulatory mechanisms in the facultatively photosynthetic bacterium Rhodobacter sphaeroides. In this study we focused on the role of the OxyR protein, a known regulator of the H(2)O(2) response in bacteria. The transcriptome profiles of R. sphaeroides wild-type and oxyR mutant strains that were exposed to 1 mM H(2)O(2) for 7 min or were not exposed to H(2)O(2) were analyzed. Three classes of OxyR-dependent genes were identified based on their expression patterns in the wild type of oxyR mutant strains with differing predicted roles of oxidized and reduced OxyR as activators of transcription. DNA binding studies revealed that OxyR binds upstream of class I genes, which are induced by H(2)O(2) and exhibit similar basal levels of expression in the wild-type and oxyR mutant strains. The effect of OxyR on class II genes, which are also induced by H(2)O(2) but exhibit significantly lower basal levels of expression in the wild-type strain than in the mutant, is indirect. Interestingly, reduced OxyR also activates expression of few genes (class III). The role of reduced OxyR as an activator is shown for the first time. Our data reveal that the OxyR-mediated response is fast and transient. In addition, we found that additional regulatory pathways are involved in the H(2)O(2) response.