]> BibSonomy publications for /author/Rossi/ag_detection http://www.bibsonomy.org/burst/author/Rossi/ag_detection BibSonomy BuRST Feed for /author/Rossi/ag_detection 2008-08-21T08:13:59+02:00 Development of a TIRF immunosensor: modelling the equilibrium behaviour of a competitive system http://www.bibsonomy.org/bibtex/22595b4e43dcf61d24ff9e5992c792ea9/biblio24 biblio24 2006-07-07T01:10:50+02:00 ag_detection competition immunosensor methodology statistic
Development of a TIRF immunosensor: modelling the equilibrium behaviour of a competitive system
Claudio Domenici and Antonio Schirone and Michele Celebre and Arti Ahluwalia and Danilo De Rossi Biosensors and Bioelectronics 10 371--378 (1995)
to ag_detection competition immunosensor methodology statistic by biblio24 on 2006-07-07 01:10:50 ]]> Biosensors and Bioelectronics3-4371--378Development of a TIRF immunosensor: modelling the equilibrium behaviour of a competitive system101995ag_detection competition immunosensor methodology statistic 2006-07-07 01:10:50.0In this work a number of instrumental aspects related to different optical configurations of Total Internal Reflection Fluoresence (TIRF) systems are introduced, and the influence of some biological reaction parameters on the performance of optical TIRF immunosensors are discussed.The manner in which fundamental quantities, such as the dissociation constant of the immunological reaction in the adsorbed phase, affect the measurement range of a TIRF immunosensor is described. The effects of light scattering at the interface and non-specific binding are also examined.Using a simple equilibrium model, in which experimental values of the surface density of active antibodies and of the dissociation constants in the immobilized phase are used, these parameters are directly related to the range of concentrations in which a competitive immunosensor gives a useful response. It is shown that amongst the most critical parameters affecting sensor performance are the dissociation constant of the antibody-antigen and antibody-labelled antigen reactions in the immobilized phase. Methods of controlling the effects of these parameters are considered.