BibSonomyburst/user/ipzgiessen/IFZBibSonomy RSS feed for /user/ipzgiessen/IFZ2012-02-16T08:14:42+01:00http://www.bibsonomy.org/bibtex/230435d7d17c44e4b3c5c09749f7e308f/ipzgiessenipzgiessen2008-06-04T14:37:55+02:00IFZ imported <span class="authorEditorList"><a href="/author/Friedt">Wolfgang Friedt</a> </span><em>ERNAHRUNGS-UMSCHAU</em> <em>54(3):108+</em> (<em>2007</em>)Wed Jun 04 14:37:55 CEST 2008ERNAHRUNGS-UMSCHAU3108+Smart breeding542007IFZ imported "Smart breeding", sometimes also called "clever breeding" or "precision breeding". stands for methodical alternatives to genetic engineering in modern plant breeding. Classical methods - such as crossbreeding of different parent varieties and Subsequent selection of progeny - are combined with novel molecular biological approaches. Smart breeding uses molecular-biological methods (e.g. gene mapping, mutagenesis) to characterize initial plants with desirable breeding properties. Methods of vegetable tissue culturing and subsequent regeneration of intact plants essentially reduce the steps among classical crossbreeding, selection of desirable breeding results and subsequent propagation. The breeding targets, such as e.g. resistance to diseases and pest infestation, stress tolerance (e.g. against dryness, salt, heat) or changed components (e.g. fatty acid pattern) are lastly achieved by means of classical breeding, i.e. by combination of suitable parent varieties, thus leading to useful, not genetically engineered plants.http://www.bibsonomy.org/bibtex/2c878c6af1b0a05ea365cdb93d343ea0a/ipzgiessenipzgiessen2008-06-04T14:34:51+02:00IFZ SSRs Triticum aestivum_L. genetic_diversity microsatellites quantitative_structure_analysis spring_bread_wheat <span class="authorEditorList"><a href="/author/Hai">Lin Hai</a>, <a href="/author/Wagner">Carola Wagner</a>, and <a href="/author/Friedt">Wolfgang Friedt</a> </span><em>GENETICA</em> <em>130(3):213-225</em> (<em>2007</em>)Wed Jun 04 14:34:51 CEST 2008GENETICA3213-225Quantitative structure analysis of genetic diversity among spring bread wheats (Triticum aestivum L.) from different geographical regions1302007IFZ SSRs Triticum aestivum_L. genetic_diversity microsatellites quantitative_structure_analysis spring_bread_wheat Genetic diversity in spring bread wheat (T.aestivum L.) was studied in a total of 69 accessions. For this purpose, 52 microsatellite (SSR) markers were used and a total of 406 alleles were detected, of which 182 (44.8%) occurred at a frequency of < 5% (rare alleles). The number of alleles per locus ranged from 2 to 14 with an average of 7.81. The largest number of alleles per locus occurred in the B genome (8.65) as compared to the A (8.43) and D (5.93) genomes, respectively. The polymorphism index content (PIC) value varied from 0.24 to 0.89 with an average of 0.68. The highest PIC for all accessions was found in the B genome (0.71) as compared to the A (0.68) and D genomes (0.63). Genetic distance-based method (standard UPGMA clustering) and a model-based method (structure analysis) were used for cluster analysis. The two methods led to analogical results. Analysis of molecular variance (AMOVA) showed that 80.6% of the total variation could be explained by the variance within the geographical groups. In comparisonhttp://www.bibsonomy.org/bibtex/288dd0f4b761e43e15111a8ebae15a09e/ipzgiessenipzgiessen2008-06-04T14:34:51+02:00IFZ imported <span class="authorEditorList"><a href="/author/Adoukonou-Sagbadja">H. Adoukonou-Sagbadja</a>, <a href="/author/Wagner">C. Wagner</a>, <a href="/author/Dansi">A. Dansi</a>, <a href="/author/Ahlemeyer">J. Ahlemeyer</a>, <a href="/author/Daienou">O. Daienou</a>, <a href="/author/Akpagana">K. Akpagana</a>, <a href="/author/Ordon">F. Ordon</a>, and <a href="/author/Friedt">W. Friedt</a> </span><em>THEORETICAL AND APPLIED GENETICS</em> <em>115(7):917-931</em> (<em>2007</em>)Wed Jun 04 14:34:51 CEST 2008THEORETICAL AND APPLIED GENETICS7917-931Genetic diversity and population differentiation of traditional fonio millet (Digitaria spp.) landraces from different agro-ecological zones of West Africa1152007IFZ imported Fonio millets (Digitaria exilis Stapf, D. iburua Stapf) are valuable indigenous staple food crops in West Africa. In order to investigate the genetic diversity and population differentiation in these millets, a total of 122 accessions from five countries (Benin, Burkina Faso, Guinea, Mali and Togo) were analysed by Amplified Fragment Length Polymorphisms (AFLPs). Genetic distance-based UPGMA clustering and principal coordinate analysis revealed a clear-cut differentiation between the two species and a clustering of D. exilis accessions in three major genetic groups fitting to their geographical origins.http://www.bibsonomy.org/bibtex/2d90451043304c4003b79a29f33446b98/ipzgiessenipzgiessen2008-06-04T14:34:51+02:00B._oleracea B._rapa Brassica_napus IFZ Verticillium_longisporum erucic_acid resistance resynthesized_rapeseed <span class="authorEditorList"><a href="/author/Rygulla">W. Rygulla</a>, <a href="/author/Friedt">W. Friedt</a>, <a href="/author/Seyis">F. Seyis</a>, <a href="/author/Luehs">W. Luehs</a>, <a href="/author/Eynck">C. Eynck</a>, <a href="/author/von Tiedemann">A. von Tiedemann</a>, and <a href="/author/Snowdon">R. J. Snowdon</a> </span><em>PLANT BREEDING</em> <em>126(6):596-602</em> (<em>2007</em>)Wed Jun 04 14:34:51 CEST 2008PLANT BREEDING6596-602Combination of resistance to Verticillium longisporum from zero erucic acid Brassica oleracea and oilseed Brassica rapa genotypes in resynthesized rapeseed (Brassica napus) lines1262007B._oleracea B._rapa Brassica_napus IFZ Verticillium_longisporum erucic_acid resistance resynthesized_rapeseed Resynthesized (RS) forms of rapeseed (Brassica napus L.; genome AACChttp://www.bibsonomy.org/bibtex/29e4259e779d518dcda2cb73e08538399/ipzgiessenipzgiessen2008-06-04T14:34:51+02:00IFZ imported <span class="authorEditorList"><a href="/author/Rygulla">W. Rygulla</a>, <a href="/author/Snowdon">R. J. Snowdon</a>, <a href="/author/Eynck">C. Eynck</a>, <a href="/author/Koopmann">B. Koopmann</a>, <a href="/author/von Tiedemann">A. von Tiedemann</a>, <a href="/author/Luehs">W. Luehs</a>, and <a href="/author/Friedt">W. Friedt</a> </span><em>PHYTOPATHOLOGY</em> <em>97(11):1391-1396</em> (<em>2007</em>)Wed Jun 04 14:34:51 CEST 2008PHYTOPATHOLOGY111391-1396Broadening the genetic basis of Verticillium longisporum resistance in Brassica napus by interspecific hybridization972007IFZ imported Verticillium wilt caused by the vascular fungal pathogen Verticillium longisporum is one of the most important pathogens of oilseed rape (Brassica napus sp. oleifera) in northern Europe. Because production of this major oilseed crop is expanding rapidly and no approved fungicides are available for V longisporum, long-term control of the disease can only be achieved with cultivars carrying effective quantitative resistance. However, very little resistance to V longisporum is available within the gene pool of oilseed rape, meaning that interspecific gene transfer from related species is the only possibility for broadening levels of resistance in current varieties. The amphidiploid species B. napus can be resyn-thesized by crossing the two progenitor species Brassica oleracea and Brassica rapa, hence resistant accessions of these two diploid species can be used as resistance donors. In this study a total of 43 potential B. rapa and B. oleracea resistance donors were tested with regard to their reaction to a mixture of two aggressive V longisporum isolates, and resistances from diverse lines were combined by embryo rescue-assisted interspecific hybridization in resynthesized rapeseed lines. Progenies from crosses of the two B. rapa gene bank accessions 13444 and 56515 to the B. oleracea gene bank accessions BRA 1008, CGN 14044, 8207, BRA 1398, and 7518 showed a broad spectrum of resistance in pathogenicity tests. Of 45 tested resynthesized lines, 41 lines exhibited a significantly higher level of resistance than the moderately V longisporum-tolerant oilseed rape cultivar Express. These lines represent a promising basis for the combination of different resistance resources in new varieties.http://www.bibsonomy.org/bibtex/23646944cbc41937e67ba28bf343491af/ipzgiessenipzgiessen2008-06-04T14:34:51+02:00Brassica_napus IFZ Oilseed_rape QTL double-low_quality heterosis locus quantitative_trait <span class="authorEditorList"><a href="/author/Basunanda">P. Basunanda</a>, <a href="/author/Spiller">T. H. Spiller</a>, <a href="/author/Hasan">M. Hasan</a>, <a href="/author/Gehringer">A. Gehringer</a>, <a href="/author/Schondelmaier">J. Schondelmaier</a>, <a href="/author/Luehs">W. Luehs</a>, <a href="/author/Friedt">W. Friedt</a>, and <a href="/author/Snowdon">R. J. Snowdon</a> </span><em>PLANT BREEDING</em> <em>126(6):581-587</em> (<em>2007</em>)Wed Jun 04 14:34:51 CEST 2008PLANT BREEDING6581-587Marker-assisted increase of genetic diversity in a double-low seed quality winter oilseed rape genetic background1262007Brassica_napus IFZ Oilseed_rape QTL double-low_quality heterosis locus quantitative_trait Generation of novel genetic diversity for maximization of heterosis in hybrid production is a significant goal in winter oilseed rape breeding. Here, we demonstrate that doubled haploid (DH) production using microspore cultivation can simultaneously introgress favourable alleles for double-low seed quality (low erucic acid and low-glucosinolate content) into a genetically diverse Brassica napus genetic background. The DH lines were derived from a cross between a double-low quality winter rapeseed variety and a genetically diverse semisynthetic B. napus line with high erucic acid and high glucosinolates (+ + quality). Twenty-three low-glucosinolate lines were identified with a genome component of 50-67% derived from the + + parent. Four of these lines, with a genome component of 50-55% derived from the + + parent, also contained low erucic acid. Heterosis for seed yield was confirmed in test-crosses using these genetically diverse lines as pollinator. The results demonstrate the potential of marker-assisted identification of novel genetic pools for breeding of double-low quality winter oilseed rape hybrids.http://www.bibsonomy.org/bibtex/2989387bba9e15d958b257418fad44fa7/ipzgiessenipzgiessen2008-06-04T14:34:51+02:00BaMMV BaYMV IFZ barley_(Hordeum_vulgare_L) doubled_haploids_(DHs) molecular_markers pyramiding resistance <span class="authorEditorList"><a href="/author/Werner">Kay Werner</a>, <a href="/author/Friedt">Wolfgang Friedt</a>, and <a href="/author/Ordon">Frank Ordon</a> </span><em>EUPHYTICA</em> <em>158(3):323-329</em> (<em>2007</em>)Wed Jun 04 14:34:51 CEST 2008EUPHYTICA3323-329Localisation and combination of resistance genes against soil-borne viruses of barley (BaMMV, BaYMV) using doubled haploids and molecular markers1582007BaMMV BaYMV IFZ barley_(Hordeum_vulgare_L) doubled_haploids_(DHs) molecular_markers pyramiding resistance Barley yellow mosaic virus disease caused by different strains of BaYMV and BaMMV is a major threat to winter barley cultivation in Europe. Different resistance genes against these viruses have been mapped and suitable PCR-based markers have been developed. In this respect doubled haploid (DH) populations proved to be advantageous as they facilitate a repeated test for resistance against all agents of the barley yellow mosaic virus complex and besides this, dominant marker systems are as informative as co-dominant ones in DHs due to the lack of heterozygous genotypes. Using DH populations resistance genes rym4, rym5, rym11, rym13, rym15 and the BaYMV/BaYMV-2 resistance of the barley cultivar �Chikurin Ibaraki 1� have been mapped. DHs are also well suited to pyramiding resistance genes against BaMMV and BaYMV. Since homozygous recessive genotypes are more frequent in DHs than in segregating F-2 populations, DHs can be efficiently used to create broad-spectrum resistance and to extend the usability of partly overcome resistance genes. Results from employing two different strategies for pyramiding, based on one and two DH-steps, respectively, combining three recessive resistance genes, i.e. rym4/rym5, rym9 and rym11, are presented. The faster strategy based on one haploidy step resulted in the identification of all three and two-way combinations of the respective resistance genes.http://www.bibsonomy.org/bibtex/26cd032d536b46854bb346d3978b48dff/ipzgiessenipzgiessen2008-06-04T14:34:51+02:00IFZ Oilseed_rape heterosis hybrids marginal_conditions <span class="authorEditorList"><a href="/author/Gehringer">Anke Gehringer</a>, <a href="/author/Snowdon">Rod Snowdon</a>, <a href="/author/Spiller">Tobias Spiller</a>, <a href="/author/Basunanda">Panjisakti Basunanda</a>, and <a href="/author/Friedt">Wolfgang Friedt</a> </span><em>BREEDING SCIENCE</em> <em>57(4):315-320</em> (<em>2007</em>)Wed Jun 04 14:34:51 CEST 2008BREEDING SCIENCE4315-320New oilseed rape (Brassica napus) hybrids with high levels of heterosis for seed yield under nutrient-poor conditions572007IFZ Oilseed_rape heterosis hybrids marginal_conditions Winter oilseed rape (Brassica napus L.) is the most important oil crop in Europe. Due to a continually increasing demand for rapeseed oil for food and non-food uses, the production of hybrid cultivars with higher seed and oil yields has become increasingly important in recent years. However, the systematic use of heterosis for hybrid breeding in oilseed rape is limited by the relatively narrow genetic basis of adapted germplasm, which can impede the generation of distinct heterotic pools. In the present study experimental hybrids were developed from a population of 190 DH lines derived from a cross between an elite, double-low seed quality (zero erucic acid, low glucosinolate content) winter oilseed rape variety and a semi-synthetic line derived from a genetically diverse resynthesised rapeseed line with high erucic acid and glucosinolate contents. The DH lines were crossed with a male sterile tester and the resulting test hybrids were examined for yield performance at two locations in Hesse, Germany, that exhibit extreme differences in climatic conditions and soil characteristics. Mid-parent heterosis for seed yield was determined at both the agronomically optimal location Rauischholzhausen and the marginal site Niederhorlen. A value of up to 43% mid-parent heterosis for seed yield could be observed among selected test hybrids compared to that of their parental DH lines. The heterosis level for yield was particularly high at the nutrient-poor site, where the best test hybrids showed significantly higher yields than elite open-pollinating and hybrid varieties. This demonstrates the suitability and adaptability of highly heterotic rapeseed hybrids on marginal locations and suggests the existence of a strong heterotic effect on nutrient uptake efficiency.http://www.bibsonomy.org/bibtex/2a4fc7805481e5b2b87e577f2e0bf2224/ipzgiessenipzgiessen2008-06-04T14:34:51+02:002C-values Digitaria_spp. Fonio IFZ West-Africa chromosome_number flow_cytometry genome_size <span class="authorEditorList"><a href="/author/Adoukonou-Sagbadja">H. Adoukonou-Sagbadja</a>, <a href="/author/Schubert">V. Schubert</a>, <a href="/author/Dansi">A. Dansi</a>, <a href="/author/Jovtchev">G. Jovtchev</a>, <a href="/author/Meister">A. Meister</a>, <a href="/author/Pistrick">K. Pistrick</a>, <a href="/author/Akpagana">K. Akpagana</a>, and <a href="/author/Friedt">W. Friedt</a> </span><em>PLANT SYSTEMATICS AND EVOLUTION</em> <em>267(1-4):163-176</em> (<em>2007</em>)Wed Jun 04 14:34:51 CEST 2008PLANT SYSTEMATICS AND EVOLUTION1-4163-176Flow cytometric analysis reveals different nuclear DNA contents in cultivated Fonio (Digitaria spp.) and some wild relatives from West-Africa26720072C-values Digitaria_spp. Fonio IFZ West-Africa chromosome_number flow_cytometry genome_size Nuclear DNA amounts of 118 cultivated fonio accessions representing 94 landraces collected from the major growing areas of West-Africa (Benin, Burkina Faso, Guinea, Mali and Togo) and eight accessions of four wild relatives were investigated by Laser flow cytometry. In cultivated species, average 2C-values ranged from 1.848 +/- 0.031 pg for Digitaria iburua to 1.956 +/- 0.004 pg for D. exilis. In D. exilis landraces thehttp://www.bibsonomy.org/bibtex/2d52cc20d6412bd122b3666be880a258f/ipzgiessenipzgiessen2008-06-04T14:34:51+02:00Blumeria_graminis CAPS-marker Hordeum_vulgare IFZ Mlg cDNA-AFLP <span class="authorEditorList"><a href="/author/Korell">M. Korell</a>, <a href="/author/Eschholz">T. W. Eschholz</a>, <a href="/author/Eckey">C. Eckey</a>, <a href="/author/Biedenkopf">D. Biedenkopf</a>, <a href="/author/Kogel">Marc K. H. Kogel</a>, <a href="/author/Friedt">W. Friedt</a>, and <a href="/author/Ordon">F. Ordon</a> </span><em>PLANT BREEDING</em> <em>127(1):102-104</em> (<em>2008</em>)Wed Jun 04 14:34:51 CEST 2008PLANT BREEDING1102-104Development of a cDNA-AFLP derived CAPS marker co-segregating with the powdery mildew resistance gene Mlg in barley1272008Blumeria_graminis CAPS-marker Hordeum_vulgare IFZ Mlg cDNA-AFLP Powdery mildew is an important pathogen of barley in many barley-growing areas. To identify molecular markers for the resistance gene Mlg located on chromosome 4H, a cDNA-AFLP approach was carried out on near-isogenic lines. Based on the identification of a differentiating fragment of 37 bp, which turned out to be part of a nucleoside diphosphate kinase as estimated by BLASTN, a CAPS marker has been developed, which co-segregated with Mlg in a population of 738 F-2-plants. Due to its co-dominant inheritance, clear banding pattern and close linkage this marker is well suited for marker-based selection procedures.