http://www.bibsonomy.org/burst/user/mikromolbio/Artificial_Gene_FusionBibSonomy BuRST Feed for /user/mikromolbio/Artificial_Gene_Fusion2008-10-16T06:43:01+02:00http://www.bibsonomy.org/bibtex/2f1831a18a9ee9dba81778273d45463ab/mikromolbiomikromolbio2008-05-07T15:52:44+02:00Amino_Acid_Sequence Gene_Expression_Regulation beta-Galactosidase Sequence_Alignment Rhodobacter_sphaeroides Bacterial_Proteins Peroxides Molecular_Sequence_Data Superoxides Deoxyribodipyrimidine_Photo-Lyase Anti-Bacterial_Agents Artificial_Gene_Fusion Singlet_Oxygen Sigma_Factor Genes IFZ Light Up-Regulation Reporter Bacterial Transcription_Factors Microbial_Viability <span style="color:#555555;">Anne-Kathrin <a href="http://www.bibsonomy.org/author/Hendrischk">Hendrischk</a> und Stephan <a href="http://www.bibsonomy.org/author/Braatsch">Braatsch</a> und Jens <a href="http://www.bibsonomy.org/author/Glaeser">Glaeser</a> und Gabriele <a href="http://www.bibsonomy.org/author/Klug">Klug</a> </span><em>Microbiology (Reading, England)</em><em>June2007. </em><em>PMID: 17526841 . </em>Wed May 07 15:52:44 CEST 2008Microbiology (Reading, England)JunePMID: 175268411842-51The phrA gene of Rhodobacter sphaeroides encodes a photolyase and is regulated by singlet oxygen and peroxide in a sigma(E)-dependent manner1532007Amino_Acid_Sequence Gene_Expression_Regulation beta-Galactosidase Sequence_Alignment Rhodobacter_sphaeroides Bacterial_Proteins Peroxides Molecular_Sequence_Data Superoxides Deoxyribodipyrimidine_Photo-Lyase Anti-Bacterial_Agents Artificial_Gene_Fusion Singlet_Oxygen Sigma_Factor Genes IFZ Light Up-Regulation Reporter Bacterial Transcription_Factors Microbial_Viability The genome of the facultatively photosynthetic bacterium Rhodobacter sphaeroides encodes three proteins of the photolyase/cryptochrome family. This paper shows that phrA (RSP2143) encodes a functional photolyase, which is an enzyme that repairs UV radiation-induced DNA damage in a blue light dependent manner. Expression of phrA is upregulated in response to light, with no photoreceptor or the photosynthetic electron transport being involved. The results reveal that singlet oxygen and hydrogen peroxide dependent signals are transmitted by the sigma(E) factor and the anti-sigma(E) factor ChrR affecting phrA expression, while superoxide anions do not stimulate phrA expression. Thus, the sigma(E) regulon is involved not only in the response to singlet oxygen but also in the hydrogen peroxide response.