http://www.bibsonomy.org/burst/user/mikromolbio/Sigma_FactorBibSonomy BuRST Feed for /user/mikromolbio/Sigma_Factor2008-10-16T06:46:11+02:00http://www.bibsonomy.org/bibtex/27ffd100e93e552721654848a86efd795/mikromolbiomikromolbio2008-05-07T15:52:44+02:00Gene_Expression_Regulation Two-Dimensional Gel Electrophoresis Host_Factor_1_Protein Rhodobacter_sphaeroides Bacterial_Proteins Reactive_Oxygen_Species Catalase Hydrogen_Peroxide Singlet_Oxygen Sigma_Factor Protein_Biosynthesis RNA Cations IFZ Proteome Light Bacterial Mutation Transcription_Factors Divalent Messenger <span style="color:#555555;">Jens <a href="http://www.bibsonomy.org/author/Glaeser">Glaeser</a> und Monica <a href="http://www.bibsonomy.org/author/Zobawa">Zobawa</a> und Friedrich <a href="http://www.bibsonomy.org/author/Lottspeich">Lottspeich</a> und Gabriele <a href="http://www.bibsonomy.org/author/Klug">Klug</a> </span><em>Journal of proteome research</em><em>July2007. </em><em>PMID: 17536848 . </em>Wed May 07 15:52:44 CEST 2008Journal of proteome researchJulyPMID: 175368482460-71Protein synthesis patterns reveal a complex regulatory response to singlet oxygen in Rhodobacter62007Gene_Expression_Regulation Two-Dimensional Gel Electrophoresis Host_Factor_1_Protein Rhodobacter_sphaeroides Bacterial_Proteins Reactive_Oxygen_Species Catalase Hydrogen_Peroxide Singlet_Oxygen Sigma_Factor Protein_Biosynthesis RNA Cations IFZ Proteome Light Bacterial Mutation Transcription_Factors Divalent Messenger Singlet oxygen (1O2) is a stress factor and signal in the facultative phototrophic bacterium Rhodobacter sphaeroides. In vivo protein labeling with L-[35S]-methionine and analysis by two-dimensional gel electrophoresis revealed that the synthesis of 61 proteins was changed in response to 1O2. After 1O2 treatment, protein synthesis patterns were distinct from those after H2O2 treatment but similar to those after high light exposure. This indicates regulatory mechanisms selective for different reactive oxygen species (ROS) and a response to light partly mediated by 1O2. Analysis of mutant strains support that the response to 1O2 is regulated mainly by rpoE (sigma E), but also a modulation of the sigma E dependent response by other factors and the existence of sigma E independent responses. The involvement of the RNA chaperon Hfq in the 1O2 response implies a role of small regulatory RNAs.http://www.bibsonomy.org/bibtex/2f1831a18a9ee9dba81778273d45463ab/mikromolbiomikromolbio2008-05-07T15:52:44+02:00Amino_Acid_Sequence Gene_Expression_Regulation beta-Galactosidase Sequence_Alignment Rhodobacter_sphaeroides Bacterial_Proteins Peroxides Molecular_Sequence_Data Superoxides Deoxyribodipyrimidine_Photo-Lyase Anti-Bacterial_Agents Artificial_Gene_Fusion Singlet_Oxygen Sigma_Factor Genes IFZ Light Up-Regulation Reporter Bacterial Transcription_Factors Microbial_Viability <span style="color:#555555;">Anne-Kathrin <a href="http://www.bibsonomy.org/author/Hendrischk">Hendrischk</a> und Stephan <a href="http://www.bibsonomy.org/author/Braatsch">Braatsch</a> und Jens <a href="http://www.bibsonomy.org/author/Glaeser">Glaeser</a> und Gabriele <a href="http://www.bibsonomy.org/author/Klug">Klug</a> </span><em>Microbiology (Reading, England)</em><em>June2007. </em><em>PMID: 17526841 . </em>Wed May 07 15:52:44 CEST 2008Microbiology (Reading, England)JunePMID: 175268411842-51The phrA gene of Rhodobacter sphaeroides encodes a photolyase and is regulated by singlet oxygen and peroxide in a sigma(E)-dependent manner1532007Amino_Acid_Sequence Gene_Expression_Regulation beta-Galactosidase Sequence_Alignment Rhodobacter_sphaeroides Bacterial_Proteins Peroxides Molecular_Sequence_Data Superoxides Deoxyribodipyrimidine_Photo-Lyase Anti-Bacterial_Agents Artificial_Gene_Fusion Singlet_Oxygen Sigma_Factor Genes IFZ Light Up-Regulation Reporter Bacterial Transcription_Factors Microbial_Viability The genome of the facultatively photosynthetic bacterium Rhodobacter sphaeroides encodes three proteins of the photolyase/cryptochrome family. This paper shows that phrA (RSP2143) encodes a functional photolyase, which is an enzyme that repairs UV radiation-induced DNA damage in a blue light dependent manner. Expression of phrA is upregulated in response to light, with no photoreceptor or the photosynthetic electron transport being involved. The results reveal that singlet oxygen and hydrogen peroxide dependent signals are transmitted by the sigma(E) factor and the anti-sigma(E) factor ChrR affecting phrA expression, while superoxide anions do not stimulate phrA expression. Thus, the sigma(E) regulon is involved not only in the response to singlet oxygen but also in the hydrogen peroxide response.