http://www.bibsonomy.org/burst/user/mikromolbio/Trans-Activators+Deoxyribonuclease_IBibSonomy BuRST Feed for /user/mikromolbio/Trans-Activators+Deoxyribonuclease_I2008-07-21T01:21:48+02:00http://www.bibsonomy.org/bibtex/2192438d916f82e78d51d7cfdd8031f65/mikromolbiomikromolbio2008-05-07T15:52:44+02:00Rhodobacter_capsulatus IFZ Oxidation-Reduction Blotting Protein_Binding Luciferases Northern Trans-Activators Electrophoresis Two-Hybrid_System_Techniques Bacterial Polyacrylamide_Gel Gene_Expression_Regulation Bacterial_Proteins Deoxyribonuclease_I Electrophoretic_Mobility_Shift_Assay <span style="color:#555555;">Jutta <a href="http://www.bibsonomy.org/author/Gregor">Gregor</a> and Tanja <a href="http://www.bibsonomy.org/author/Zeller">Zeller</a> and Angelika <a href="http://www.bibsonomy.org/author/Balzer">Balzer</a> and Kerstin <a href="http://www.bibsonomy.org/author/Haberzettl">Haberzettl</a> and Gabriele <a href="http://www.bibsonomy.org/author/Klug">Klug</a> </span><em>Journal of molecular microbiology and biotechnology</em>(<em>2007</em>) <em>PMID: 17693720 . </em>Wed May 07 15:52:44 CEST 2008Journal of molecular microbiology and biotechnologyPMID: 17693720126-39Bacterial regulatory networks include direct contact of response regulator proteins: interaction of RegA and NtrX in Rhodobacter capsulatus132007Rhodobacter_capsulatus IFZ Oxidation-Reduction Blotting Protein_Binding Luciferases Northern Trans-Activators Electrophoresis Two-Hybrid_System_Techniques Bacterial Polyacrylamide_Gel Gene_Expression_Regulation Bacterial_Proteins Deoxyribonuclease_I Electrophoretic_Mobility_Shift_Assay The formation of photosynthetic complexes in facultatively photosynthetic bacteria is controlled by the oxygen tension in the environment. In Rhodobacter capsulatus the two-component system RegB/RegA plays a major role in the redox control of photosynthesis genes but also controls other redox-dependent systems. The response regulator RegA is phosphorylated under low oxygen tension and activates the puf and puc operons, which encode pigment binding proteins, by binding to their promoter regions. Data from a yeast two-hybrid analysis as well as an in vitroanalysis indicate that RegA interacts with the NtrX protein, the response regulator of the NtrY/NtrX two-component system which is believed to be involved in regulation of nitrogen fixation genes. Our further analysis revealed that NtrX is indeed involved in the regulation of the puf and puc operons. Furthermore, we showed that an altered NtrX protein, which is predicted to adopt the conformation of phosphorylated NtrX protein, binds within the puf promoter region close to the RegA binding sites. We conclude that a direct interaction of two response regulators connects the regulatory systems for redox control and nitrogen control.